SAN TA C RUZ BI OTEC HNOL OG Y, INC . MUL1 (T-13): sc-160072 BACKGROUND APPLICATIONS MUL1 (mitochondrial E3 ubiquitin protein ligase 1), also known as GIDE, MUL1 (T-13) is recommended for detection of MUL1 of mouse, rat and MAPL, MULAN or RNF218, is a 352 amino acid multi-pass mitochondrial human origin by Western Blotting (starting dilution 1:200, dilution range outer membrane protein that is widely expressed with highest levels in 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein heart, skeletal muscle, placenta, kidney and liver. Existing as a homooligo- (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution mer, MUL1 contains one RING-type zinc finger, which is required for E3 lig - range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution ase activity. MUL1 acts as an E3 ubiquitin-protein ligase that participates in range 1:30-1:3000). the control of mitochondrial morphology, promotes mitochondrial fragmenta - MUL1 (T-13) is also recommended for detection of MUL1 in additional tion and influences mitochondrial localization. Overexpression of MUL1 acti - species, including canine, bovine and porcine. vates JNK (c Jun N terminal kinase) through Tak1 (transforming growth fac - Suitable for use as control antibody for MUL1 siRNA (h): sc-78840, MUL1 tor β-activated kinase 1) and induces caspase-dependent apoptosis. MUL1 is encoded by a gene located on human chromosome 1, which spans 260 siRNA (m): sc-149706, MUL1 shRNA Plasmid (h): sc-78840-SH, MUL1 shRNA million base pairs, contains over 3,000 genes, comprises nearly 8% of the Plasmid (m): sc-149706-SH, MUL1 shRNA (h) Lentiviral Particles: sc-78840-V human genome and houses a large number of disease-associated gene. and MUL1 shRNA (m) Lentiviral Particles: sc-149706-V. Molecular Weight of MUL1: 40 kDa. REFERENCES Positive Controls: KNRK whole cell lysate: sc-2214. 1. Matsuda, A., et al. 2003. Large-scale identification and characterization of human genes that activate NF B and MAPK signaling pathways. κ RECOMMENDED SECONDARY REAGENTS Oncogene 22: 3307-3318. To ensure optimal results, the following support (secondary) reagents are 2. Ardley, H.C. and Robinson, P.A. 2005. E3 ubiquitin ligases. Essays recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 Biochem. 41: 15-30. (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey 3. Zhang, B., et al. 2008. GIDE is a mitochondrial E3 ubiquitin ligase that anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ induces apoptosis and slows growth. Cell Res. 18: 900-910. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- 4. Neuspiel, M., et al. 2008. Cargo-selected transport from the mitochondria itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). to peroxisomes is mediated by vesicular carriers. Curr. Biol. 18: 102-108. 3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution 5. Li, W., et al. 2008. Genome-wide and functional annotation of human E3 range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: ubiquitin ligases identifies MULAN, a mitochondrial E3 that regulates the 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. organelle's dynamics and signaling. PLoS ONE 3: e1487. DATA CHROMOSOMAL LOCATION Genetic locus: MUL1 (human) mapping to 1p36.12; Mul1 (mouse) mapping 90K– to 4 D3. 55K– SOURCE 43K– < MUL1 MUL1 (T-13) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of MUL1 of human origin. 34K– PRODUCT MUL1 (T-13): sc-160072. Westernblot analysisof MUL1 expressioninKNRK whole cell lysate. Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. PROTOCOLS Blocking peptide available for competition studies, sc-160072 P, (100 µg See our web site at www.scbt.com or our catalog for detailed protocols and peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). support products. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. RESEARCH USE For research use only, not for use in diagnostic procedures. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.