FBXO5 polyclonal antibody (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box Catalog Number: PAB10207 proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, Regulatory Status: For research use only (RUO) and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The Product Description: Rabbit polyclonal antibody raised protein encoded by this gene belongs to the Fbxs class. against synthetic peptide of FBXO5. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Immunogen: A synthetic peptide corresponding to Cdc20 and inhibits the anaphase promoting complex. N-terminus of human FBXO5. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by Host: Rabbit RefSeq] Reactivity: Chimpanzee,Dog,Human References: Applications: ELISA, IHC-P, WB-Ce 1. Plk1 regulates activation of the anaphase promoting (See our web site product page for detailed applications complex by phosphorylating and triggering information) SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1. Hansen DV, Loktev AV, Ban KH, Jackson Protocols: See our web site at PK. Mol Biol Cell. 2004 Dec;15(12):5623-34. Epub 2004 http://www.abnova.com/support/protocols.asp or product Oct 6. page for detailed protocols 2. E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1). Hsu JY, Reimann Form: Liquid JD, Sorensen CS, Lukas J, Jackson PK. Nat Cell Biol. 2002 May;4(5):358-66. Recommend Usage: ELISA (1:5000-1:20000) 3. Emi1 is required for cytostatic factor arrest in Western Blot (1:500-1:3000) vertebrate eggs. Reimann JD, Jackson PK. Nature. 2002 Immunohistochemistry (1:500-1:3000) Apr 25;416(6883):850-4. The optimal working dilution should be determined by the end user. Storage Buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) Storage Instruction: Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 26271 Gene Symbol: FBXO5 Gene Alias: EMI1, FBX5, Fbxo31 Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs Page 1/1 Powered by TCPDF (www.tcpdf.org).