Product Information Sheet for NR-602 Brevibacillus parabrevis, Strain BG Washington, DC: U.S. Government Printing Office, 2007; see www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm. Catalog No. NR-602 Disclaimers: (Derived from ATCC® 8185™) You are authorized to use this product for research use only. It is not intended for human use. For research only. Not for human use. Use of this product is subject to the terms and conditions of Contributor: the BEI Resources Material Transfer Agreement (MTA). The ATCC® MTA is available on our Web site at www.beiresources.org. Product Description: While BEI Resources uses reasonable efforts to include Bacteria Classification: Paenibacillaceae, Brevibacillus accurate and up-to-date information on this product sheet, ® Agent: Brevibacillus parabrevis neither ATCC nor the U.S. Government make any Strain: BG (NRS 751) warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for ® Material Provided: informational purposes only. Neither ATCC nor the U.S. Each vial contains approximately 0.5 mL of bacterial culture Government warrants that such information has been in 0.5X Tryptic Soy Broth supplemented with 10% glycerol. confirmed to be accurate. Packaging/Storage: This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. NR-602 was packaged aseptically, in screw-capped plastic ® cryovials. The product is provided frozen and should be ATCC and the U.S. Government are not liable for any stored at -60°C or colder immediately upon arrival. For damages or injuries arising from receipt and/or use of this long-term storage, the vapor phase of a liquid nitrogen product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. freezer is recommended. Freeze-thaw cycles should be ® avoided. Government, ATCC , their suppliers and contributors to BEI Resources are not liable for damages arising from the Growth Conditions: misidentification or misrepresentation of products. Media: Tryptic Soy Broth or equivalent Use Restrictions: Tryptic Soy Agar or equivalent This material is distributed for internal research, non- Incubation: commercial purposes only. This material, its product or its Temperature: 30°C derivatives may not be distributed to third parties. Except as Atmosphere: Aerobic performed under a U.S. Government contract, individuals Propagation: contemplating commercial use of the material, its products or 1. Keep vial frozen until ready for use; then thaw. its derivatives must contact the contributor to determine if a 2. Transfer the entire thawed aliquot into a single tube of license is required. U.S. Government contractors may need broth. a license before first commercial sale. 3. Use several drops of the suspension to inoculate an agar slant and/or plate. References: 4. Incubate the slant and/or plate at 30°C for 24 hours. 1. Shida, O., et al. “Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.” Citation: Int. J. Syst. Bacteriol. 46 (1996): 939–946. PubMed: Acknowledgment for publications should read “The following 8863420. reagent was obtained through the NIH Biodefense and 2. Takagi, H., et al. “Characterization of Bacillus brevis with Emerging Infections Research Resources Repository, NIAID, Descriptions of Bacillus migulanus sp. nov., Bacillus NIH: Brevibacillus parabrevis, Strain BG, NR-602.” choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov.” Int. J. Syst. Bacteriol. 43 Biosafety Level: 1 (1993): 221–231. PubMed: 8494737. 3. Dubos, R. J. and R. D. Hotchkiss. “The Production of Appropriate safety procedures should always be used with Bactericidal Substances by Aerobic Sporulating Bacilli.” this material. Laboratory safety is discussed in the following J. Exp. Med. 73 (1941): 629–640. publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and ® ATCC is a trademark of the American Type Culture Prevention, and National Institutes of Health. Biosafety in Collection. Microbiological and Biomedical Laboratories. 5th ed. Biodefense and Emerging Infections Research Resources Repository 800-359-7370 P.O. Box 4137 Fax: 703-365-2898 Manassas, VA 20108-4137 USA E-mail: [email protected] www.beiresources.org © 2007 American Type Culture Collection (ATCC). All rights reserved. NR-602_10JUL2007 Page 1 of 1 .