MAIMUUTTUNUT US009809653B2 HIU LI HUWULUHAN (12 ) United States Patent ( 10 ) Patent No. : US 9 ,809 ,653 B2 Baudat et al. (45 ) Date of Patent: Nov . 7 , 2017 (54 ) ANTI- LAMP1 ANTIBODIES AND ANTIBODY ( 58) Field of Classification Search DRUG CONJUGATES , AND USES THEREOF None See application file for complete search history . ( 71 ) Applicant: SANOFI, Paris (FR ) (72 ) Inventors: Yves Baudat, Paris (FR ) ; Francis ( 56 ) References Cited Blanche, Paris ( FR ) ; Béatrice Cameron , Paris (FR ) ; Tarik Dabdoubi, U . S . PATENT DOCUMENTS Le Coudray Montceaux (FR ) ; 4 , 179 , 337 A 12 / 1979 Davis et al. Anne -Marie LeFebvre , Le Plessis Pâté 4 ,256 ,746 A 3 / 1981 Miyashita et al . ( FR ) ; Magali Mathieu , Choisy le Roi 4 , 294 , 757 A 10 / 1981 Asai 4 , 301 , 144 A 11/ 1981 Iwashita et al. (FR ) ; Ana Merino - Trigo , Paris (FR ) ; 4 , 307 ,016 A 12 / 1981 Asai et al. 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OTHER PUBLICATIONS George et al. (Circulation . 1998 ; 97 : 900 -906 ). * ( 30 ) Foreign Application Priority Data (Continued ) Dec . 27 , 2012 ( EP ) . 12306691 Dec. 27 , 2012 (EP ) .. .. 12306694 Primary Examiner — Sheela J Huff (51 ) Int . Ci. ( 74 ) Attorney , Agent, or Firm — Sandra A . Brockman -Lee A61K 39 / 395 (2006 .01 ) CO7K 16 / 28 ( 2006 . 01 ) A61K 47 / 48 ( 2006 .01 ) (57 ) ABSTRACT GOIN 33 /574 ( 2006 .01 ) Antibodies are provided which specifically bind human and COOK 16 / 30 ( 2006 .01 ) C120 1 / 68 ( 2006 . 01 ) Macaca fascicularis lysosomal- associated membrane pro A61K 39/ 00 ( 2006 .01 ) tein 1 (LAMP1 ) proteins and immunoconjugates comprising ( 52 ) U . S . CI. said antibodies conjugated or linked to a growth inhibitory CPC . .. CO7K 16 /2896 ( 2013 .01 ) ; A61K 47/ 48384 agent . Pharmaceutical compositions comprising antibodies (2013 .01 ) ; A61K 47 /48561 ( 2013 . 01 ) ; A61K or immunoconjugates of the invention and use of the anti 47 /48569 (2013 .01 ) ; A61K 47 / 48584 bodies or immunoconjugates for the treatment of cancer are ( 2013 .01 ) ; A61K 47/ 48592 (2013 .01 ) ; A6IK also provided , as well as LAMP1 antibodies , isolated 47/ 48615 ( 2013 .01 ) ; CO7K 16 / 30 (2013 .01 ) ; nucleic acids, vectors and host cells comprising a sequence CO7K 16 / 3023 ( 2013 .01 ) ; C07K 16 / 3046 encoding said antibodies and the use of said antibody as a ( 2013 . 01 ) ; CO7K 16 /3069 (2013 .01 ) ; C120 diagnostic tool. The application further provides for the 1 /6886 ( 2013 .01 ) ; GOIN 33 /574 ( 2013 .01 ) ; detection of LAMP1 gene amplification or gain in cancer A61K 2039 / 505 ( 2013 .01 ) ; COZK 2317 / 24 cells leading to the determination if patients with cancer are ( 2013 . 01 ) ; CO7K 2317 / 33 ( 2013 .01 ) ; COOK likely to respond to anti -LAMP1 therapy. Anti- LAMP1 2317 /34 ( 2013 . 01 ) ; COZK 2317 /41 ( 2013 .01 ) ; therapeutic agent for use for treating cancer in a patient CO7K 2317/ 565 ( 2013 .01 ) ; CO7K 2317 / 73 harboring LAMP1 gene copy number gain in cancer cells is (2013 .01 ) ; CO7K 2317 / 732 ( 2013 .01 ) ; COOK further provided . 2317 / 77 ( 2013 .01 ) ; CO7K 2317 / 92 ( 2013 .01 ) ; C12Q 2600 /106 ( 2013. 01 ) ; C12Q 2600/ 156 (2013 .01 ) 17 Claims, 47 Drawing Sheets US 9 ,809 , 653 B2 Page 2 (56 ) References Cited Anonymous, Anti LAMP1 Human MaxPab (H00003916 -B01P ) , tebu -bio , retrieved from http : / /www .tebu -bio .com /Product / U . S . PATENT DOCUMENTS 157H00003916 - 1301P on Jul. 11, 2014 . Anonymous, APC anti -human CD107a (LAMP - 1 ) Product Data 4 ,670 ,417 A 6 / 1987 Iwasaki et al. 4 , 791 , 192 A 12 / 1988 Nakagawa et al. 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