Quantification of Ethyl Carbamate in Tobacco

Quantification of Ethyl Carbamate in Tobacco

QUANTIFICATION OF ETHYL CARBAMATE IN TOBACCO POSTER-15 Dinesh.T.K., Sathya Gourishankar and Sharad.K.Mehta* ITC Ltd, ITC R&D Centre, Peenya, Bangalore – 560 058, INDIA. ABSTRACT SAMPLE PREPARATION SAMPLE CHROMATOGRAM REPRODUCIBILITY ETHYL 2.5g Sample CARBAMATE ANALYST TRIAL NO. SAMPLE (ng/g) 2012_TSRC15_Mehta.pdf A simple method is developed for quantification of Ethyl 1 Tobacco 371.0 carbamate in Tobacco. Ethyl carbamate in Tobacco is 2 Tobacco 368.7 extracted with water and is partitioned with Sonicate with 25ml water for 30 mins and 3 Tobacco 382.2 dichloromethane. The dichloromethane extract is Centrifuge at 10000 rpm for 10 minutes. 4 Tobacco 374.1 concentrated and injected in GC/MS-SIM mode. The 1 5 Tobacco 384.1 method has been validated using standard validation 6 Tobacco 389.0 protocols and there is excellent linearity over 7 Tobacco 368.3 concentration range from 100 to 4000 ng/g with Extract 10 ml of water extract with 30ml correlation coefficient of 0.9939. The recoveries are more dichloromethane twice. 8 Tobacco 367.1 than 90% for Ethyl carbamate with limit of quantification 9 Tobacco 364.5 100 ng/g and limit of detection 40ng/g respectively. carbamate Ethyl 2 10 Tobacco 372.2 Concentrate the dichloromethane extract STDEV 8.2 after passing through sodium sulphate and MEAN 374.1 inject in GC-MS. %RSD 2.2 INTRODUCTION GC-MS CONDITIONS LINEARITY RECOVERY STUDIES CALIBRATION FOR ETHYL CARBAMATE 400000 Ethyl carbamate (Urethane) is a genotoxic carcinogen found 350000 in fermented foods and beverages. Ethyl carbamate in y = 340.35x - 5869.2 SPIKED OBTAINED Tobacco is formed from Maleic hydrazide, which is a plant • Column : Zebron-ZB Wax plus 30mX0.25mmX0.25m. R² = 0.9939 TRIAL AMOUNT AMOUNT % 300000 growth regulator applied to Tobacco. Ethyl carbamate is the • Column temperature : 80°C(2 mins.) 2°C per min. to NO. SAMPLE (ng/g) (ng/g) RECOVERY R 130°C(2 mins.) 20°C per min. ethyl ester of carbamic acid. It is also formed from E 250000 hydrogen cyanide and ethanol or urea and ethanol. Although to 220°C(3 mins.). S P there are a number of analytical methods reported in the • Injector temperature: 200°C. 200000 O 1 Tobacco 403 459 114 literature, they all have two major drawbacks, namely, (1) • Transfer Line Temperature: 210°C. N Requires sophisticated instruments like UPLC/MS/MS and • Injection volume: 1.0 µl. S 150000 E (2) Need laborious clean up procedure to remove the matrix • Scan Range: 50 – 550 amu. effect of Tobacco. The aim of the method is to develop a • Quantification Ions: 62, 74 & 89. 100000 2 Tobacco 403 449 111 simple method with less clean up to remove matrix and use 50000 routine instrument like GCMS to quantify Ethyl carbamate in Tobacco. 0 0 200 400 600 800 1000 1200 CONC. IN PPM 3 Tobacco 403 389 96.5 LITERATURE STANDARD CHROMATOGRAM REPEATABILITY CONCLUSIONS METHOD REFERENCES LIMITATIONS ETHYL • Laborious extraction A Formation and Determination of CARBAMATE procedure. Ethyl carbamate in Tobacco and TRIAL NO. SAMPLE (ng/g) Tobacco Smoke, Schmeltz, Chiong • Matrix interference. and Hoffmann. J Analytical Toxicology 2(1978) 265-268. 1 Tobacco 371.0 • A simple method for quantification B Ethyl carbamate levels in US and • Sophisticated Swedish smokeless tobacco products. instrumentation 2 Tobacco 368.7 of Ethyl carbamate in Tobacco is developed. (LC-MS/MS). Faizi A., Kimpton H., McAdam K., BAT, Group Research and Development, Southampton, UK. 3 Tobacco 382.2 • Recovery of greater than 95%. University of Louisville, Louisville, USA. SSPTPOST14, CORESTA 4 Tobacco 374.0 Conference, Edinburgh September 2010. 5 Tobacco 384.1 • Method for stone-fruit. C Application of tandem mass spectrometry combined with gas chromatography to the routine STDEV 6.8 analysis of ethyl carbamate in stone- Ethyl carbamate Ethyl fruit spirits. Dirik W.Lachenmeier*, MEAN 376.0 Willi Frank and Thomas Kuballa. Rapid Commun.Mass Spectrom.2005; 19: 108-112. %RSD 1.8 TSRC2012(66) - Document not peer-reviewed.

View Full Text

Details

  • File Type
    pdf
  • Upload Time
    -
  • Content Languages
    English
  • Upload User
    Anonymous/Not logged-in
  • File Pages
    1 Page
  • File Size
    -

Download

Channel Download Status
Express Download Enable

Copyright

We respect the copyrights and intellectual property rights of all users. All uploaded documents are either original works of the uploader or authorized works of the rightful owners.

  • Not to be reproduced or distributed without explicit permission.
  • Not used for commercial purposes outside of approved use cases.
  • Not used to infringe on the rights of the original creators.
  • If you believe any content infringes your copyright, please contact us immediately.

Support

For help with questions, suggestions, or problems, please contact us