list list sample) short list predicted B number Operon ID Gene name assignment Protein length mRNA present mRNA intensity Gene description Protein detected - Strand orientation Membrane protein detected (total list) detected (long list) membrane sample Proteins detected - detected (short list) # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides Functional category detected (membrane Protein detected - total Protein detected - long b0001 thrL + 21 1344 P 1 0 0 0 0 thr operon leader peptide Metabolism of small molecules 1 b0002 thrA + 820 13624 P 39 P 18 P 18 P 18 P(m) 2 aspartokinase I, homoserine dehydrogenase I Metabolism of small molecules 1 b0003 thrB + 310 6781 P 9 P 3 3 P 3 0 homoserine kinase Metabolism of small molecules 1 b0004 thrC + 428 15039 P 18 P 10 P 11 P 10 0 threonine synthase Metabolism of small molecules 1 b0005 b0005 + 98 432 A 5 0 0 0 0 orf, hypothetical protein Open reading frames 2 b0006 yaaA - 258 1047 P 11 P 1 2 P 1 0 orf, hypothetical protein Open reading frames 3 b0007 yaaJ - 476 342 P 8 0 0 0 0 MP-GenProt-PHD inner membrane transport protein Miscellaneous 4 b0008 talB + 317 20561 P 20 P 13 P 16 P 13 0 transaldolase B Metabolism of small molecules 5 b0009 mog + 195 1296 P 7 0 0 0 0 required for the efficient incorporation of molybdate into molybdoproteins Metabolism of small molecules 6 b0010 yaaH - 188 407 A 2 0 0 0 0 PHD orf, hypothetical protein Open reading frames 7 b0011 b0011 - 237 338 P 13 0 0 0 0 putative oxidoreductase Miscellaneous 8 b0012 htgA + 196 149 P 11 0 0 0 0 positive regulator for sigma 32 heat shock promoters Cell processes 9 b0013 yaaI - 134 288 A 7 0 0 0 0 orf, hypothetical protein Open reading frames 10 b0014 dnaK + 638 13283 P 32 P 23 P 24 P 23 0 chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins Cell processes 11 b0015 dnaJ + 376 4492 P 13 P 4 P 6 P 4 P(m) 1 chaperone with DnaK; heat shock protein Cell processes 11 b0016 yi81_1 + 370 17 0 0 0 0 IS186 hypothetical protein Extrachromosomal 12 b0017 yi82_1 - 102 135 P 6 0 0 0 0 IS186 and IS421 hypothetical protein Extrachromosomal 13 b0018 gef - 69 215 P 3 0 0 0 0 Gef protein interferes with membrane function when in excess Cell processes 14 b0019 nhaA + 388 1174 P 7 0 0 0 0 GenProt-PHD Na+/H antiporter, pH dependent Cell processes 15 b0020 nhaR + 301 645 P 13 0 0 0 0 transcriptional activator of nhaA Cell processes 16 b0021 insB_1 - 167 9 0 0 0 0 IS1 protein InsB Extrachromosomal 17 b0022 insA_1 - 91 5 0 0 0 0 IS1 protein InsA Extrachromosomal 17 b0023 rpsT - 87 7837 P 3 P 2 2 P 2 0 30S ribosomal subunit protein S20 Structural elements 18 b0024 b0024 + 72 163 P 4 0 0 0 0 PHD orf, hypothetical protein Open reading frames 19 b0025 ribF + 313 3873 P 15 0 0 0 0 putative regulator Metabolism of small molecules 19 list list sample) short list predicted B number Operon ID Gene name assignment Protein length mRNA present mRNA intensity Gene description Protein detected - Strand orientation Membrane protein detected (total list) detected (long list) membrane sample Proteins detected - detected (short list) # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides Functional category detected (membrane Protein detected - total Protein detected - long b0026 ileS + 938 4036 P 46 P 13 P 14 P 13 0 isoleucine tRNA synthetase Macromolecule metabolism 20 b0027 lspA + 164 3643 P 6 0 0 0 0 GenProt-PHD prolipoprotein signal peptidase (SPase II) Macromolecule metabolism 20 probable FKBX-type 16KD peptidyl-prolyl cis-trans isomerase (a b0028 slpA + 149 2825 P 5 P 1 2 P 1 0 rotamase) Macromolecule metabolism 21 b0029 lytB + 316 1331 P 16 P 2 2 P 2 0 control of stringent response; involved in penicillin tolerance Global functions 20 b0030 yaaF + 304 685 P 8 0 0 0 0 orf, hypothetical protein Open reading frames 22 b0031 dapB + 273 2348 P 16 P 3 3 P 3 0 dihydrodipicolinate reductase Metabolism of small molecules 23 b0032 carA + 382 9312 P 14 P 8 P 8 P 8 0 carbamoyl-phosphate synthetase, glutamine (small) subunit Metabolism of small molecules 24 b0033 carB + 1073 7656 P 48 P 17 P 19 P 17 0 carbamoyl-phosphate synthase large subunit Metabolism of small molecules 24 b0034 caiF + 166 813 P 9 0 0 0 0 transcriptional regulator of cai operon Metabolism of small molecules 25 b0035 caiE - 203 190 P 7 0 0 0 0 possible synthesis of cofactor for carnitine racemase and dehydratase Metabolism of small molecules 26 b0036 caiD - 297 476 P 14 0 0 0 0 carnitine racemase Metabolism of small molecules 26 b0037 caiC - 522 285 P 25 0 0 0 0 GenProt-PHD probable crotonobetaine/carnitine-CoA ligase Metabolism of small molecules 26 b0038 caiB - 405 291 P 19 0 0 0 0 l-carnitine dehydratase Metabolism of small molecules 26 b0039 caiA - 380 308 A 18 0 0 0 0 probable carnitine operon oxidoreductase Metabolism of small molecules 26 b0040 caiT - 504 148 A 5 0 0 0 0 GenProt-PHD probable carnitine transporter Metabolism of small molecules 26 b0041 fixA + 268 105 A 6 0 0 0 0 probable flavoprotein subunit, carnitine metabolism Metabolism of small molecules 27 b0042 fixB + 313 365 P 14 0 0 0 0 probable flavoprotein subunit, carnitine metabolism Metabolism of small molecules 27 b0043 fixC + 428 215 A 19 0 0 0 0 flavoprotein; electron transport Metabolism of small molecules 27 b0044 fixX + 95 135 A 7 0 0 0 0 putative ferredoxin Metabolism of small molecules 27 b0045 yaaU + 443 133 P 7 0 0 0 0 GenProt-PHD putative transport protein Cell processes 28 b0046 yabF + 176 356 P 6 0 0 0 0 putative NAD(P)H oxidoreductase Metabolism of small molecules 29 b0047 kefC + 620 543 P 29 0 0 0 0 GenProt-PHD K+ efflux antiporter, glutathione-regulated Cell processes 29 b0048 folA + 159 1588 P 7 P 1 2 P 1 0 dihydrofolate reductase type I; trimethoprim resistance Metabolism of small molecules 30 b0049 apaH - 280 703 P 11 P 2 1 P 2 0 diadenosine tetraphosphatase Metabolism of small molecules 31 b0050 apaG - 125 2140 P 5 0 0 0 0 orf, hypothetical protein Open reading frames 32 list list sample) short list predicted B number Operon ID Gene name assignment Protein length mRNA present mRNA intensity Gene description Protein detected - Strand orientation Membrane protein detected (total list) detected (long list) membrane sample Proteins detected - detected (short list) # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides Functional category detected (membrane Protein detected - total Protein detected - long b0051 ksgA - 273 2240 P 10 P 1 2 P 1 0 S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase Cell processes 32 b0052 pdxA - 329 1543 P 13 0 0 0 0 pyridoxine biosynthesis Metabolism of small molecules 32 peptidyl-prolyl cis-trans isomerase (PPIase), involved in maturation of b0053 surA - 428 3825 P 19 P 4 P 5 P 4 P(m) 1 GenProt outer membrane proteins (1st module) Cell processes 32 b0054 imp - 784 2737 P 42 P 5 0 0 P(m) 5 GenProt organic solvent tolerance Cell processes 33 b0055 yabH + 271 797 P 16 0 0 0 0 putative DNA binding protein Miscellaneous 34 b0056 yabP + 216 470 P 12 0 0 0 0 orf, hypothetical protein Open reading frames 35 b0057 yabQ + 52 327 P 3 0 0 0 0 orf, hypothetical protein Open reading frames 35 b0058 yabO - 219 525 P 8 0 0 0 0 orf, hypothetical protein Macromolecule metabolism 36 b0059 hepA - 968 1379 P 44 P 2 2 P 2 0 probable ATP-dependent RNA helicase Macromolecule metabolism 36 b0060 polB - 783 350 P 40 0 0 0 0 DNA polymerase II Macromolecule metabolism 37 b0061 araD - 231 571 P 8 0 0 0 0 L-ribulose-5-phosphate 4-epimerase Metabolism of small molecules 38 b0062 araA - 500 268 P 17 0 0 0 0 L-arabinose isomerase Metabolism of small molecules 38 b0063 araB - 566 307 P 20 0 0 0 0 L-ribulokinase Metabolism of small molecules 38 b0064 araC + 292 704 P 10 0 0 0 0 transcriptional regulator for ara operon Metabolism of small molecules 39 b0065 yabI + 254 396 A 6 0 0 0 0 PHD orf, hypothetical protein Open reading frames 40 b0066 yabJ - 232 458 P 12 0 0 0 0 putative ATP-binding component of a transport system Cell processes 41 b0067 yabK - 536 774 P 14 0 0 0 0 GenProt-PHD putative transport system permease protein Cell processes 41 b0068 tbpA - 327 957 P 13 P 1 1 P 1 0 thiamin-binding periplasmic protein Cell processes 41 b0069 yabN - 551 472 P 27 0 0 0 0 putative transport protein Cell processes 42 b0070 setA + 392 218 A 7 0 0 0 0 GenProt-PHD Sugar efflux protein Cell processes 43 b0071 leuD - 201 4770 P 10 P 9 P 7 P 9 0 isopropylmalate isomerase subunit Metabolism of small molecules 44 b0072 leuC - 466 10437 P 18 P 9 P 7 P 9 0 3-isopropylmalate isomerase (dehydratase) subunit Metabolism of small molecules 44 b0073 leuB - 364 10273 P 12 P 8 P 8 P 8 0 3-isopropylmalate dehydrogenase Metabolism of small molecules 44 b0074 leuA - 523 11143 P 28 P 7 P 7 P 7 0 2-isopropylmalate synthase Metabolism of small molecules 44 b0075 leuL - 28 1458 P 1 0 0 0 0 leu operon leader peptide Metabolism of small molecules 44 list list sample) short list predicted B number Operon ID Gene name assignment Protein length mRNA present mRNA intensity Gene description Protein detected - Strand orientation Membrane protein detected (total list) detected (long list) membrane sample Proteins detected - detected (short list) # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides Functional category detected (membrane Protein detected - total Protein detected - long b0076 leuO + 373 158 P 15 0 0 0 0 probable transcriptional activator for leuABCD operon Metabolism of small molecules