Red clover casebearer (Coleophora deauratella) monitoring in the Peace River Region Otani, J.1, Jorgensen, A.1 1 Agriculture & Agri-Food Canada (AAFC), Beaverlodge, AB Background 2014-004 Red 2nd-yr Q: Does RCCB larval abundance and development differ on red compared to alsike flower heads? The red clover casebearer moth (RCCB), Coleophora deauratella Lieneg & Zeller (Lepidoptera: Coleophoridae), is an invasive pest Methods: 2014-004 Mountain Trail 1 first found in the Peace River region in 2005 . RCCB can cause Hand collected flower heads from fields and ditches near nd 2 seed yield losses of ≥80% in 2 -year seed stands of red clover . Falher, AB (N=50 buds/species/site*; Table 1). 2 RCCB also utilizes Alsike clover . Individually monitored flowers for larval emergence (Fig. 4). RCCB is univoltine with moths commencing flight in mid-June in 2,3 the Peace River region. Eggs are laid on the calyx of florets . 2014-046 Red 1st-yr The larvae feed within then amongst the florets from June to late August then overwinter in field trash2,3. Monitoring Sites 2014-046 Lavadiere A B Fig. 4. Red clover flowers reared individually (A) and RCCB larva feeding within floret (B). 2014-048 Red 1st-yr Table 1. Density of Coleophora sp. larvae in red and alsike flower heads (N=50 flowers/patch)*sampled from seed stands and adjacent volunteer clover plants on July 23, 2014. 2014-048 Rochon Patch Number of larvae Density (Number of Site Crop Location size(m2) per patch larvae per flower)* Red Field 50 40 0.8 Site 2014- 049 Alsike Ditch 15 0 0 2014-049 Red 1st-yr Red Field 50 5 0.1 Site 2014-046 Alsike Ditch 5 0 0 RCCB Males / Day / Trap / Day Males RCCB Red Ditch 10 278 5.56 Site 2014-047 Fig. 1. Sites used for pheromone and sweep-net monitoring and to determine Alsike Field 50 0 0 larval abundance. Map legend: Red=Red clover, Yellow=Alsike Clover, Red Field 50 15 0.3 Green=wheat underseeded to red clover. Site 2014-048 Alsike Ditch 10 0 0 2014-049 Chaibos Red 160 338 0.8±0.14a Total Alsike 80 0 0±0.00a a Value represents mean ± standard error of mean. a 2014-050 Red Underseeded In Progress Photo: S. Dufton b 2014-050 Houle Fig. 2. Green unitrap4 in a clover field. Q: What are the seasonal adult populations in clover fields? D AAFC-OTANI2014-047 Alsike 1st-yr Methods: 4 Pheromone traps (Fig. 2; N=2 per site) deployed at 6 2 mm 1 mm 1.5 mm Albertan sites (Fig. 1 & 3; N=5 sites near Falher, N=1 site E near Beaverlodge). A B C 2014-047 Limoge Sweep-net monitoring at 6 sites (Fig. 2; N=100 Fig. 5. Magnified views of RCCB larva including a Mature case (A), a larva within sweeps/week). its case (B), a larva extracted from its case (C), the dissected head capsule (D) Traps contents and sweeps collected weekly; identified and the rear prolegs adapted to grip its case (E). Photos: AAFC-Jorgensen. moths to species and verified sex ratio in sweep-net samples Week of Year (in progress). Fig. 3. Pheromone trap catches per day per trap in 2014. Agriculture and Agri-Food Canada Acknowledgements Technical support was provided by Kayleigh Loberg, Shelby Dufton, Holly Spence, Sara Stagg, Kaitlin Freeman, Jadin Chahade and Graham Fonseca. Producer Cooperators: Agriculture et Agroalimentaire Canada Terry Chaibos, Lucien Houle, Norman Lavadiere, Danny Limoge, Marc Rochon. References : (1) J. Otani unpublished data . (2) Ellis & Bjørnson. 1996. Proc. Ent. Soc. Ont. 127:115-124. (3). Hammer. 1937. Jou. Pla. Bre. 42 (2):333-343 (4). https://www.contech-inc.com/ (5) Photo courtesy of J-F. Landry. .