AQUATIC RESEARCH Abbreviation: Aquat Res e-ISSN: 2618-6365 journal published in one volume of four issues per year by www.ScientificWebJournals.com Aims and Scope “AQUATIC RESEARCH" journal publishes peer-reviewed articles covering all aspects of Aquatic Biology, Aquatic Ecology, Aquatic Environment and Pollutants, Aquaculture, Conservation and Management of Aquatic Source, Economics and Managements of Fisheries, Fish Diseases and Health, Fisheries Resources and Management, Genetics of Aquatic Organisms, Limnology, Maritime Sciences, Marine Accidents, Marine Navigation and Safety, Marine and Coastal Ecology, Oseanography, Seafood Processing and Quality Control, Seafood Safety Systems, Sustainability in Marine and Freshwater Systems in the form of review articles, original articles, and short communications. Peer-reviewed (with two blind reviewers) open access journal publishes articles quarterly in English or Turkish language. © 2018 ScientificWebJournals (SWJ) All rights reserved/Bütün hakları saklıdır. I Chief Editor: Prof. Dr. Nuray ERKAN, [email protected] Istanbul University, Faculty of Aquatic Sciences, Department Seafood Processing Technology and Safety, Turkey Cover photo: Prof. Dr. Sühendan MOL, [email protected] Istanbul University, Faculty of Aquatic Sciences, Department Seafood Processing Technology and Safety, Turkey Editorial Board: Prof. Dr. Miguel Vazquez ARCHDALE, [email protected] Kagoshima University, Faculty of Fisheries Fisheries Resource Sciences Department, Japan Prof. Dr. Mazlan Abd. GHAFFAR, [email protected]; [email protected] University of Malaysia Terengganu, Institute of Oceanography and Environmental, Kuala Nerus Terengganu, Malaysia Prof. Dr. Adrian GROZEA, [email protected] Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, Timisoara, Romania Prof. Dr. Saleem MUSTAFA, [email protected] University of Malaysia Sabah, Borneo Marine Research Institute, Malaysia Prof. Dr. Tamuka NHIWATIWA, [email protected] University of Zimbabwe, Department of Biological Sciences, Harare, Zimbabwe Prof. Dr. Özkan ÖZDEN, [email protected] Istanbul University, Faculty of Aquatic Sciences, Department Seafood Processing Technology and Safety, Turkey Prof. Dr. Murat YİĞİT, [email protected] Canakkale Onsekiz Mart University, Faculty of Marine Science and Technology, Çanakkale, Turkey Assoc. Prof. Dr. Makiko ENOKI, [email protected] Tokyo University of Marine Science and Technology Faculty of Marine Science, Department of Marine Reources and Energy, Japan Assoc. Prof. Dr. Athanasios EXADACTYLOS, [email protected] University of Thessaly (UTh), Department of Ichthyology and Aquatic Environment (DIAE), Volos, Greece Assoc. Prof. Dr. Rossen STEFANOV, [email protected] Institute of Biology and Immunology of Reproduction, Sofia, Bulgaria Assoc. Prof. Dr. Matthew TAN, [email protected] James Cook University, Centre for Sustainable Tropical Fisheries and Aquaculture (CSTFA) - College of Science & Engineering, Australia II Vol. 1 Issue 3 Page 103-139 (2018) Table of Contents/İçerik 1. GENETIC DIVERSITY OF THE ENDEMIC SPECIES SHABBOUT (Arabibarbus grypus (HECKEL, 1843)) BASED ON PARTIAL CYTOCHROME B SEQUENCES OF MITOCHONDRIAL DNA Pages 103-109 Arif Parmaksız, Özlem Şeker 2. EFFECT OF SAVORY EXTRACT SUPPLEMENTATION ON SOME PRODUCTIVE TRAITS AND ECONOMIC EFFICIENCY OF COMMON CARP (Cyprinus carpio L.) Pages 110-114 Stefka Stoyanova, Georgi Zhelyazkov, Katya Velichkova, Ivaylo Sirakov, Yordan Staykov 3. FILLETING YIELD, BODY CHARACTERISTICS AND LENGTH WEIGHT RELATIONSHIP OF FOUR FISH SPECIES FROM LOWER RIVER BENUE MAKURDI NIGERIA Pages 115-126 Simon Ihie Ikape, Shola Gabriel Solomon 4. GÜNEYDOĞU KARADENİZ RİZE SARAYKÖY PLAJINDA PLASTİK KİRLİLİĞİ Pages 127-135 Fatma Başak Esensoy Şahin, Furkan Karacan, Ülgen Aytan 5. BEHAVIOR AND RESPONSE OF JAPANESE CATFISH (Silurus asotus) IN CAPTIVITY PROVIDED WITH AN ARTIFICIAL MICROHABITAT MOSAIC Pages 136-139 Nur Efah Arsin, Abentin Estim, Saleem Mustafa III E-ISSN 2618-6365 Aquatic Research 1(3), 103-109 (2018) • DOI: 10.3153/AR18011 Original Article/Full Paper GENETIC DIVERSITY OF THE ENDEMIC SPECIES SHABBOUT (Arabibarbus grypus (HECKEL, 1843)) BASED ON PARTIAL CYTOCHROME B SEQUENCES OF MITOCHONDRIAL DNA Arif Parmaksız , Özlem Şeker Cite this article as: Parmaksız, A., Şeker, Ö. (2018). Genetic Diversity of the Endemic Species Shabbout (Arabibarbus grypus (Heckel, 1843)) Based on Partial Cytochrome B Sequences of Mitochondrial DNA. Aquatic Research, 1(3), 103-109. DOI: 10.3153/AR18011 Harran University, Faculty of Science and Art, Department of Biology, ABSTRACT 63100, Şanlıurfa, Turkey Arabibarbus grypus (Heckel, 1843), a species endemic in river systems of Euphrates and Tigris, is an economically important freshwater fish. In this study, the genetic diversity of Arabibarbus grypus populations was determined basen on partial cytochrome b gene sequence of mtDNA. Totally 31 samples were collected from four localities and five polymorphic sites and five haplotypes were identified by carrying out mtDNA analysis. Mean haplotype (Hd) and nucleotide diversity (π) were calculated to be 0.348 and 0.00144 respectively. All values obtained following neutrality tests were found to be negative and statistically insignificant. Median joining network revealed that haplotype Submitted: 12.02.2018 H1 was at the center of the network and was dominant. In the current survey, certain haplotypes Accepted: 09.03.2018 (H2, H4, H5) identified for mtDNA cytochrome b gene are the new results to the literature and presented a novel data set for genetic diversity of this species. Published online: 12.03.2018 Keywords: Arabibarbus grypus, Cytochrome b, Genetic diversity, Euphrates River, Tigris River Correspondence: Arif PARMAKSIZ E-mail: [email protected] ©Copyright 2018 by ScientificWebJournals Available online at http://aquatres.scientificwebjournals.com 103 Aquatic Research 1(3), 103-109 (2018) • DOI: 10.3153/AR18011 Original Article/Full Paper Introduction Euphrates and Tigris Rivers are from important natural The aim of this research is to determine genetic diversity of sources of fish diversity and fishing and also possess a con- A. grypus populations in Euphrates and Tigris rivers via se- siderable potential for meeting the need of food. Developed quence analysis for mtDNA Cyt b fragment. countries have started to conduct overall studies about spe- cies particularly with economic importance following the Materials and Methods classification of fish in inland waters (Kaya, 2012). A Collection of fish samples: A total of 31 individuals (15 from majority of fish species inhabiting in basin of Euphrates and Euphrates and 16 from Tigris River) were collected via fish- Tigris belongs to the family Cyprinidae. Because several ing method. 2 g of specimen was dissected from muscle tis- species of this family are consumed for food, they posses sue on the base of pectoral or dorsal fins of fish samples, economic importance (Parmaksız et al., 2016). The fish held in refrigerator at 4°C inside micro centrifuge tubes with preferred most by local people thanks to its delicious meat 1.5 mL volume, containing 95% ethanol until DNA isolation is Arabibarbus grypus (Shabbout). The fish distributed in process. Iran, Turkey, Syria, and Iraq is an endemic species thriving in river systems of Euphrates and Tigris (Nikpei, 1996; DNA isolation: Total DNA was isolated from muscular tis- Abdoli, 2000; Khodadadi et al., 2016). Endemic fish species sue using GeneJET Genomic DNA Purification Kit of fish are important in terms of ecological aspects and as- (Thermo Scientific). Total DNA was obtained by practiced sumed as gene banks of an ecosystem (Khodadadi et al., the protocol for the kit. To control the existence of DNA, 2 2016). μl was taken from DNA samples of each individual, placed in to tank including 0.8% agarose gel, 0.5xTBE (Tris/Boric Some studies conducted on this species include age, growth, acid/EDTA Buffer) solution with the addition of 2 μl of stain and reproductive traits (Oymak et al., 2008); heavy metal (3x Loading dye) and SYBR Green, run in electrophoresis concentration in tissues (Oymak et al., 2009); determination at 120 Volts for 30 minutes, then viewed in device giving of spermatological and hematologic characteristics (Dogu et off ultraviolet (UV) light (SmartView Pro Imager System, al., 2014); the relationships between sagittal otolith size and Major Science). length of the fish (Dusukcan et al., 2015); investigation for concentration of mercury in edible muscle tissues (Asefi and Amplification of target mtDNA site via polymerase chain re- Zamani-Ahmadmahmoodi, 2015); the effects of probiotics action (PCR): Primers used for amplification of mtDNA derived from Lactobacillus species on immunologic param- Cytochrome b gene in the study (Briolay et al., 1998) were eters of Shabbout (Mohammadian et al., 2016); sperm mor- given below: phology, motility and composition of seminal plasma pa- L15267: 5’-AATGACTTGAAGAACCACCGT-3’ rameters (Khodadadi et al., 2016); determination of genetic H15891: 5’-GTTTGATCCCGTTTCGTGTA-3’ diversity utilizing from gene sequences of mtDNA COI (Parmaksiz et al., 2017). The PCR amplification process was carried out in a BIO- RAD T100TM Thermal Cycler under the following condi- Despite Oymak et al., (2009) stated in their study that Shab- tions: 3 minute at 95°C for initial denaturation and 30 sec- bout was abundant in the Euphrates, the number of individ- onds at 95°C for