Catalog X ISO 9001:2000 Certified 1801 Commerce Drive • South Bend, Indiana 46628 • 800-729-5270 • www.enzymeresearch.com Human Coagulation Bovine Coagulation Zymogens Zymogens • Human Prothrombin • Bovine Protein C • Human Factor VII • Bovine Prothrombin • Human Protein C • Bovine Factor IX • Human Factor IX • Bovine Factor X • Human Factor X • Bovine Plasminogen • Human Factor XI • Human Factor XII Enzymes • Human Factor XIII • Human Prekallikrein • Bovine Activated Protein C • Sing. Chain High Molecular Wt. Kininogen • Bovine Alpha-Thrombin • Two Chain High Molecular Wt. Kininogen • Bovine Factor IXa Alpha • Human Glu-Plasminogen • Bovine Factor IXa Beta • Human Lys-Plasminogen • Bovine Factor Xa • Bovine Factor XIa Enzymes CoFactors • Human Alpha Thrombin • Human Gamma Thrombin • Bovine Factor V/Va • Human Factor VIIa • Human Activated Protein C Inhibitors • Human Factor IXa Alpha • Human Factor IXa Beta • • Human Factor Xa Bovine Antithrombin • Human Factor XIa • Human Factor Alpha-XIIa Platelets and Plasma Proteins • Human Factor XIIIa • Human Kallikrein • Bovine Fibrinogen, Plasminogen Depleted • Human Plasmin Antibodies CoFactors • Monoclonal • Human Protein S • Polyclonal • Human Protein Z • Paired Antibodies • Human Fibronectin Inhibitors Venoms • Human Antithrombin • Russell's Viper Venom • Human Heparin CoFactor II • Corn Trypsin Inhibitor Other Species Proteins • a2 Antiplasmin • TAFI • Canine Proteins • Murine Proteins Platelets & Plasma Proteins • Porcine Proteins • Rabbit Proteins • Human Fibrinogen 1 2 & 3 • Rat Proteins • Human GPIIbIIIa • Apolipoprotein • Platelet Factor 4 Worldwide Distributors Des-Gla & Inactivated Proteins Technical Information • Des-Gla Proteins • Inactivated Proteins For orders in the United States, please contact our U.S. office. All other orders may be placed through the nearest distributor listed below. United States United Kingdom Enzyme Research Laboratories, Ltd. Enzyme Research Laboratories, Inc. 15 Sketty Road, Swansea, UK SA2 OEU United Kingdom 1801 Commerce Drive, South Bend, IN 46628 Tel: +44 1792 466166 • Fax: +44 1792 466366 Tel: 574-288-2268 • Fax: 574-288-2272 Contact: Sales Department • Email: [email protected] Contact: [email protected] Website: www.enzymeresearch.co.uk Australia Japan Banksia Scientific Cosmo Bio Company Unit 3, 160 Lytton Rd (P.O. Box 529) 2-2-20, Toyo Koto-ku Bulimba QLD 4171 Australia Tokyo, 135 Japan Tel: +61 7 3902 3000 • Fax: +61 7 3217 9869 Tel: 813 5632 9610 • Fax: 813 5632 9619 Free call 1300 769944 Contact: Support Team Contact: Mr. Stephen Edwards Email: [email protected] Email: [email protected] Website: www.cosmobio.co.jp Website: www.banksiascientific.com.au Austria Switzerland Coa. Chrom Diagnostica, GmbH Milan Analytica, AG 1080 Vienna, Austria Obere Egg 2, CH-4312 Magden, Switzerland Tel: +43 1 699 97 97 • Fax: +43 1 699 18 97 Tel. +41 61 845 9988 • Fax. +41 61 845 9987 Contact: Mr. Bernhard Kolmer Contact: Dr. Alfred Lampart Email: [email protected] Email: [email protected] Website: www.coachrom.com Website: www.milananalytica.ch Finland, Norway, Sweden Diagnostica & Analys Service AB the Netherlands, Belgium, France, Carl Wilhelmsonsvag 7 Switzerland, Denmark SE 416 56 Goteburg Sweden Kordia - PO Box 4018 Tel. +46 31 25 26 28 • Fax. +46 31 21 26 28 NL-2301 RA Leiden, the Netherlands Contact: Dr. Petter Friberger Tel. +31 71 523 10 50 • Fax. +31 71 522 17 00 Email: [email protected] Contact: Edward van Poort and Erik van Megen Website: www.dia-service.se Email: [email protected] Website: www.kordia.nl or www.kordia.com Germany Haemochrom Diagnostica GmbH Belgium: Kordia - PO Box 4018 Renteilichtung 1 NL-2301 RA Leiden, the Netherlands Essen, Germany DE-45134 Tel. Bruxelles: 02-42-685-12 Tel. +49 201 843 770 • Fax. +49 201 536 456 Fax Bruxelles: 02-42-801-09 Contact: Dr. Birgit Bakowski-Enzian Email: [email protected] Email: [email protected] Website: www.kordia.be Website: www.haemochrom.de Israel France: Kordia - PO Box 4018 Tarom Applied Technologies Ltd. NL-2301 RA Leiden, Pays Bas Petach Tikva Israel 49517 Tel. France: 0380 20 69 93 Tel. +972 3 9214145 • Fax.+972 3 9214146 Fax France: 0380 20 69 94 Contact: Mr. Zvi Barr Email: [email protected] Email: [email protected] Website: www.kordia.fr Website: www.tarom.co.il Italy Cabru s.a.s. Via Forlanini 52 20043 Arcore (M ) Italy B Tel. +39 039 60 13 988 • Fax. +39 039 60- 14 284 Contact: Dr. Bruno Casagrande Email: [email protected] Website: www.cabru.it TECHNICAL INFORMATION Protein Handling Enzyme Research Laboratories, Inc. makes the following suggestions for the storage and handling of purified proteins 1. Most purified proteins are stable at low temperatures and should be stored at <-60°C. 2. Lyophilized proteins should be reconstituted using a good laboratory quality H2O. After reconstitution the protein should be aliquoted into a useful (one time use) size and stored at <-60°C. 3. Freeze proteins in a concentrated form and as soon as possible. When dilution is necessary, dilute immediately prior to use. When possible, dilution buffers should contain stabilizers such as albumin. 4. Limit thawing and refreezing. Multiple thaw/freezes can reduce protein activity and cause degradation. We always suggest aliquoting the protein into a single use size. 5. Refrigerate thawed proteins or store on ice. Proteins warmed even to room temperature may degrade quickly. Refreeze proteins as soon as possible. 6. One exception to # 5 is Fibrinogen. After thawing Fibrinogen, keep at room temperature. Fibrinogen will come out of solution if it is cooled. 7. Products provided in Glycerol/buffer solutions should be stored at -20°C. Temperatures below -30°C can cause phase transitions and need to be avoided. Unit Definition 1 PEU is equal to the amount of activity found in mL of plasma as determined in factor deficient clotting assay. All extinction coefficients are reported for 280 nm using 1 cm path length. Shipping Information Unless otherwise noted, all the coagulation factors are stored at <-60°C and shipped on dry ice by overnight carrier. Shipping will be billed to customer for orders under $2000. All Human Source material is tested and found negative for all communicable diseases, including HIV-1 and HIV-2 antibodies, HCV, Rapid Plasma Reagin and Hepatitis B Surface Antigen. Because no test method can offer complete assurance that Human Immunodeficiency Virus (HIV), Hepatitis B virus or other infectious agents are absent, all human proteins should be handled as suggested in the U.S. Department of Health and Human Services Manual on Biosafety in Microbiological & Biomedical Laboratories. Back to Index Human Prothrombin HP 1002 Human Prothrombin (Factor II) Prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000, and consists of a single polypeptide chain. Activation of Prothrombin by Factor Va, Xa and phospholipids yields the serine protease Thrombin. Prothrombin purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4 *Extinction Coefficient (1%) = 13.6 One unit = 90 µg Molecular Weight =72,000 daltons Also available: des-Gla-Human Prothrombin and Prothrombin Fragment 1 and 2 Human Factor VII HFVII 1007 Human Factor VII (Proconvertin) Prepared from fresh frozen human plasma. Human Factor VII is a single-chain vitamin K dependent glycoprotein found in trace quantities in plasma (0.5 mg/Liter). In the Tissue Factor Pathway of coagulation, Human Factor VIIa, in the presence of calcium ions and tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa. Buffer composition =20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 13.9 Molecular Weight = 50,000 daltons Human Protein C HPC 1001 Human Protein C Purified from fresh frozen human plasma using a combination of salt precipitations and column chromatography. The protein purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Protein C is activated to the serine protease, Activated Protein C (APC), by a-thrombin or the complex of a-thrombin/thrombomodulin and is a potent anticoagulant through the selective inactivation of Factors Va and VIIIa. Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 14.5 Molecular Weight = 62,000 daltons Also available: des-Gla-Human Protein C Back to Table of Contents Human Factor IX HFIX 1009 Human Factor IX (Christmas Factor) Prepared from fresh frozen human plasma. This protein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor IX, activated by either the Contact or Tissue Factor Pathway, is responsible for the activation of Factor X to Xa. Buffer composition: 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) = 13.2 One unit = 5 µg Molecular Weight = 56,000 daltons Human Factor X HFX 1010 Human Factor X (Stuart Prower Factor) Prepared from fresh frozen human plasma. The purity of this Vitamin K dependent protein is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay. Human Factor X, once activated via the Contact Factor Pathway or the Tissue Factor Pathway, is responsible for the conversion of Prothrombin to Thrombin. Buffer composition 20 mM Tris-HCl/0.1 M NaCl /1 mM Benzamidine/pH 7.4 *Extinction Coefficient (1%) =11.6 One unit =8 µg Molecular Weight =58,800 daltons Also available: des-Gla-Human Factor Human Factor XI HFXI 1111 Human Factor XI (Plasma Thromboplastin Antecedent) Prepared from fresh frozen human plasma. Human Factor XI is a two-chain glycoprotein with molecular weight of 160,000 daltons. The two chains are identical disulfide bonded polypeptides with molecular weights of 80,000 daltons.