Anti-CSTF3 (C-terminal) produced in rabbit, affinity isolated antibody Product Number C9998 Product Description Antibody concentration: 1.0 mg/mL Anti-CSTF3 (C-terminal) is produced in rabbit using as the immunogen a synthetic peptide corresponding to a Precautions and Disclaimer sequence at the C-terminal of human CSTF3 (GeneID: For R&D use only. Not for drug, household, or other 1479) conjugated to KLH. The corresponding sequence uses. Please consult the Safety Data Sheet for is identical in mouse. The antibody is affinity-purified information regarding hazards and safe handling using the immunizing peptide immobilized on agarose. practices. Anti-CSTF3 (C-terminal) specifically recognizes human Storage/Stability CSTF3 (also known as Cleavage stimulation factor 77, Store at –20 C. For continuous use, the product may CSTF-77, CF-1 77 kDa subunit). The antibody may be be stored at 2–8 C for up to one month. For extended used in several immunochemical techniques including storage, freeze in working aliquots at –20 C. immunoblotting (77 kDa), immunoprecipitation, and Repeated freezing and thawing, or storage in “frost- immunofluorescence. Staining of the CSTF3 band in free” freezers, is not recommended. If slight turbidity immunoblotting is specifically inhibited with the occurs upon prolonged storage, clarify the solution by immunizing peptide. centrifugation before use. Working dilutions should be discarded if not used within 12 hours. mRNA precursors are processed 3-ends in a two-step reaction; endonucleolytic cleavage at the poly(A) site Product Profile followed by the addition of adenylate residues to form Immunoblotting: a working antibody concentration of a poly(A) tail. The mammalian mRNA 3 end 0.5-1 g/mL is recommended using lysates of processing complex contains several sub-complexes. HEK-293T cells. These include the cleavage-polyadenylation specificity factor (CPSF), which recognizes the nearly ubiquitous Immunoprecipitation: a working antibody amount of AAUAAA signal, cleavage stimulatory factor (CstF), 2.5-5 g is recommended using lysates of HEK-293T which interacts with a less-conserved G/U-rich cells. sequence situated downstream of the cleavage site, cleavage factors I and II (CFI and CFII) and poly(A) Immunofluorescence: a working antibody polymerase (PAP). concentration of 1.25-2.5 g/mL is recommended using paraformaldehyde fixed HeLa cells. CstF is a heterotrimer consisting of subunits of 50, 64, and 77 kDa referred to as CSTF1, CSTF2, and Note: In order to obtain best results in various CSTF3, respectively. CSTF3 is required for proper techniques and preparations, it is recommended to 3-end cleavage and is critical for the assembly of the determine optimal working dilutions by titration. CstF complex, bridging both CSTF1 and CSTF2. It contains 12 HAT repeats followed by a proline-rich References segment. CSTF3 also interact directly with the RNA 1. Zhao, J., et al., Microbiol. Mol. Biol. Rev., 63,405- polymerase II CTD, but with less efficiency than 445 (1998). 1-2 CSTF1. 2. Mandel, C.R., et al., Cell. Mol. Life Sci., 65, 1099- 1122 (2008). Reagent Supplied as a solution in 0.01 M phosphate buffered VS,SG,TD,KAA,PHC,MAM 04/19-1 saline, pH 7.4, containing 15 mM sodium azide as a preservative. 2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .