Puławy 20.10.2016 Potwierdzenie wystąpienia afrykańskiego pomoru świń ;ASF w Polsce przez Europejskie Laboratorium Referencyjne (EURL) ds. ASF w Valdeolmos w Hiszpanii dla przypadku nr 42 oraz przypadków od 75 do 105 oraz ogniska nr 4 (1/2016). ir,,rr,ll,.;:'il,, i li rj:,1!' i,,,r_i I iii, itt; i;t,r riirl:r;i r ;L::.ijr,Tr,,::,r $ MINISTERIO i;ilriir ii t r'):rl r" ,:l i¡r il li r il l : i¡j DE ECONOMÍA Y l:i ¡]" [- ! !,] ¡\ i-^ -' **-^'-***'-"-''t Instilurr: Nacio¡lal dc lnvrsrigar:irirr COMPETITIVIDAD ,l y Ir'<'nulo¡¡ia .{¡iraria y r\linrenraria SUBDIRECCION GENERAL DE ENSAYfTS INVESTIGACIÓN Y TECNOLOGíA N" 9ó.9/LEl865 CENTRO DE INVESTIGACIÓN _ EN SANIDAD ANIMAL * Assays and activities mariett with are not supported by ENAC acueditation. characterization REPORT ISSUED BY THE EUROPEAN UNION REFERENCE LABORATORY FOR AFRICAN swtNE FEVER (EURL-ASF), tNtA-CrSA DATE: September 23'o, 2016. ARRfVAI DATE TO CISA: July 22"o,2016. RESPONSIBTE FOR SUBMISSION: Name: Andrzej Kowalczyk Institution/Lab: National Veterinary Research Inst¡tute (NVRI) Address: 57 Partyzantow Avenue,24-tOO PULAWY Country: POTAND Tel. number: +48 81889 3047 Fax number: +48 81 886 2595 Num. ARRIVAI REGISTER CISA: REG. L44/2016. TEST REQUESTED: AFRICAN SWINE FEVER (ASF) CONFIRMA'I-ORY DIAGNOSIS AND GENE SEQUENCING. Num. SAMPIES RECEIVED: 86 samples from 40 wild boars comprising 20 tissues, 14 homogenates of tissues, 14 bloods and 38 DNAs.45 samples from 9 domestic pigs comprising 5 bloods, 18 tissues and 23 DNAs identified as follows (table 1): WILD BOAR 19L2I 7 cAsE 75 I972r/t5 Bialowie2a TISSU E BONE MARROW WILD BOAR 3to7Llts 2 cAsE 79 3LO7L f{arewka TISSUE BONE MARROW WILD BOAR 32842tts 3 CASE 80 32842 llarewka TISSUE BONE MARROW WILD BOAR 3298r/L5 4 cAsE 81 3298r Biatowie2a TISSUE BONE MARROW WILD BOAR 00969/16 cAsE 85 00969 l\arewka T SSUE SPLEEN WILD BOAR 00969/16 6 cAsE 85 00969 l\arewka TISSUE LYMPHNODE WILD BOAR 00969/16 7 cAsE 85 00969 l\arewka TISSUE KIDNEY Ctra, de Algete a El Casar, s/n Page 1 of 13 28130 Valdeolmos MADRID TEL: 91 620 23 00 FAX: 91 6202247 MINISTERIO DE ECONOMfA Y Ilrstil u¡o Nacional do lflvÉ:itigarión COMPETITIVIDAD 'li.< y rrrrlo¡¡Ía Agraria y i\lirrrcnraria i. '; $ii ? SUBDIRECCIÓN GENERAL DE INVESTIGACIÓN Y TECNOLOGh CENTRO DE INVESTIGACIÓN EN SANIDADANIMAL WILD BOAR 05778/L6 8 cAsE 89 05778 Narewka T SSUE BONE MARROW WILD BOAR 0698s/16 9 CASE 90 0698s Biatowieia TISSUE BONE MARROW WILD BOAR 0753L/L6 10 cAsE 91 0763L B¡afowieia TISSUE BONE MARROW WILD BOAR LO37s/L6 LL cAsE 93 LO375 Narewka TISSUE BONE MARROW WILD BOAR I2990t16 L2 cAsE 94 12990 Narewka TISSUE BONE MARROW WILD BOAR L40ss/16 I5 cAsE 95 14055 Biatowie2a HOMOGENATE BONE MARROW WILD BOAR t40s6/L6 L4 CASE 95 14056 B¡aiowie2a HOMOGENATE BONE MARROW DOMESTIC PIG ts337/t6 15 OUTBREAK 4 15337 Hainówka TISSUE K DNEY DOMESTIC PIG L5337 116 IO OUTBREAK 4 15337 Hajnówka TISSUE SPLEEN DOMESTIC PIG 1s338/16 L7 OUTBREAK 4 15338 Hainówka T SSUE K DNEY DOMESTIC PIG 1s338/16 18 OUTBREAK 4 15338 Hajnówka TISSUE SPLEEN DOMESTIC PIG Ls339t76 19 OUTBREAK 4 15339 Hajnówka T SSUE KIDNEY DOMESTIC PIG 15339/16 20 OUTBREAK 4 15339 Hainówka TISSUE SPLEEN DOMESTIC PIG 75340/L6 2T OUTBREAK 4 15340 Hainówka TISSUE K DNEY DOMESTIC PIG Ls34O/16 22 OUTBREAK 4 15340 Hajnówka TISSUE SPLEEN DOMESTIC PIG r534U].6 z5 OUTBREAK 4 15341 Hainówka T SSUE KIDNEY DOMESTIC PIG Ls34t/t6 24 OUTBREAK 4 15341 Hajnówka TISSUE SPLEEN DOMESTIC PIG 15553/16 25 OUTBREAK 4 15553 llainówka TISSUE KIDNEY DOMESTIC PIG 15553/16 ¿o OUTBREAK 4 IJJf,5 Hainówka TISSUE SPLEEN DOMESTIC PIG t5554/t6 27 OUTBREAK 4 15554 Hainówka TISSUE KIDNEY DOMESTIC PIG L5ss4/L6 28 OUTBREAK 4 15554 Hainówka TISSUE SPLEEN DOMESTIC PIG 15sss/16 ZJ OUTBREAK 4 1555s Hainówka T SSUE K DNEY DOMESTIC PIG lssss/16 30 OUTBREAK 4 15555 Hainówka TISSU E SPLEEN DOMESTIC PIG L5556/16 5l OUTBREAK 4 15556 l{ainówka TISSU E KIDNEY DOMESTIC PIG 1sss6/16 5Z OUTBREAK 4 15556 llainówka TISSUE SPLEEN WILD BOAR Ls777/L6 33 cAsE 96 L5777 Czy2e HOMOGENATE BONE MARROW WILD BOAR 15778116 34 cAsE 96 15778 Czvie HOMOGENATE BONE MARROW WILD BOAR 15779tL6 35 cAsE 96 15779 Czv2e HOMOGENATE BONE MARROW WILD BOAR r5780/L6 5b cAsE 95 15780 Czv2e HOMOGENATE BONE MARROW WILD BOAR r5780/L6 cAsE 95 15780 Czy2e TISSUE SPLEEN WILD BOAR L6O8O/16 38 CASE 98 16080 B¡afowie¿a HOMOGENATE BONE MARROW WILD BOAR 16554/L6 39 cAsE 99 16554 llainówka HOMOGENATE BONE MARROW WILD BOAR 16555/16 40 cAsE 99 16555 l-lajnówka TISSUE SPLEEN WILD BOAR L65ss/L6 4I cAsE 99 16555 llajnówka TISSUE KIDNEY WILD BOAR t6557 /t6 42 CASE 99 L6557 Hainówka T SSUE SPLEEN WILD BOAR L6557 116 43 CASE 99 16557 l-lainówka TISSUE KIDNEY WILD BOAR 16677 lL6 44 cAsE 101 L6677 TISSUE KIDNEY WILD BOAR t6677/L6 45 CASE 101 LOOt I TISSUE SPLEEN WILD BOAR L68s8/L6 46 CASE 102 16858 l-lainówka TISSUE BONE MARROW WILD BOAR 17073tt6 47 CASE 103 L7OL3 flainówka HOMOGENATE BONE MARROW WILD BOAR L70L4l16 48 cAsE 104 t7074 flainówka HOMOGENATE BONE MARROW WILD BOAR L7jLs/t6 49 cAsE 104 t7075 flainówka HOMOGENATE BONE MARROW WILD BOAR 770t6/16 50 cAsE 104 t7016 Flainówka HOMOGENATE BONE MARROW WILD BOAR 17LO7 lL6 51 CASE 105 17L07 Flainówka HOMOGENATE BONE MARROW WILD BOAR 05097/\s JZ CASE 42 05097 Gródek HOMOGENATE BONE MARROW WILD BOAR L976s-t7/rs )5 CASE 75 19765 -WB Ne 17 Krvnki BLOOD BLOOD-EDTA WILD BOAR 27L52-55tt5 54 CASE 77 27152- WB Ne 55 Gródek BLOOD BLOOD-EDTA WILD BOAR 3029GLO/Ls 55 CASE 78 30296 - WB Ne 10 S dra BLOOD BLOOD-EDTA WILD BOAR 332s6-22lLs CASE 83 33256 - WB Ne 22 Gródek BLOOD BLOOD-EDTA WILD BOAR 3324s-3/75 57 cAsE 82 33245- WB Ne 3 Narew BLOOD BLOOD-EDTA WILD BOAR 00347-22/t6 58 cAsE 84 00347 WB Ne 22 Narew BLOOD BLOOD-EDTA WILD BOAR 00969/16 59 CASE 85 00969 Narewka BLOOD BLOOD-EDTA WILD BOAR 03s18-8/16 60 CASE 86 03518 - WB Ne 8 Narewka BLOOD BLOOD-EDTA WILD BOAR o45Itt6 OI CASE 87 04513 Supraó1, BLOOD BLOOD-EDTA WILD BOAR o5432-r2lL6 62 cAsE 88 05432 WB Ne 12 B¡afow¡e¿a BLOOD BLOOD-EDTA WILD BOAR 08s90-u16 o5 cAsE 92 08590 - WB Ne r 1 Michatowo BLOOD BLOOD-EDTA Ctra de Algete a Ef Casar, s/n Page 2 of 13 28130 Valdeolmos MADRID TEL: 91 620 23 00 FAX: 91 6202247 s.Jl, MINISTERIO DE ECONOMIA Y Ilrstiluto Nat:io¡ral r.le Invrsrigar:lnrr COMPETITIVIDAD y lccnoln¡¡ía dgraria y Alirncntaria SUBDIRECCIÓN GENERAL DE INVESTIGACIÓN Y TECNOLOGÍA CENTRO DE I¡¡VESTIGACIÓ¡. EN SANIDAD¡,NIMAL * Assays and activities marked with ore not supported' by ENAC accreditotion. DOMESTIC PIG Ls337/16 64 OUTBREAK 4 15337 Hainówka BLOOD BLOOD-EDTA DOMESTIC PIG 1s338/16 OUTBREAK 4 15338 Hainówka BTOOD BLOOD-EDTA DOMESTIC PIG L5339/16 66 OUTBREAK 4 15339 Hainówka BLOOD BLOOD.EDTA DOMESTIC PIG r534O/L6 67 OUTBREAK 4 15340 Hainówka BLOOD BLOOD-EDTA DOMESTIC PIG t534r/L6 68 OUTBREAK 4 t5347 Hajnówka BLOOD BLOOD-EDTA WILD BOAR L5887-3t16 69 cAsE 97 15887 WB Ne 3 Narewka BLOOD BLOOD-EDTA WILD BOAR L6565-7/t6 70 cAsE 100 16565 WB Ne 7 Hainówka BLOOD BLOOD-EDTA WILD BOAR 16677 /L6 7T cAsE 101 LOOT I Narewka BLOOD BLOOD-EDTA WILD BOAR L9IzL 72 cAsE 75 L9L2L|15 Bialowie2a DNA BONE MARROW WILD BOAR 3707U15 73 cAsE 79 3to77 Narewka DNA BONE MARROW WILD BOAR 32842/75 74 cAsE 80 32842 Narewka DNA BONE MARROW WILD BOAR 3298L1t5 75 CASE 81 3298t Biatowie2a DNA BONE MARROW WILD BOAR 33245-3/Ls 76 cAsE 82 33245- WB Ne 3 Narew DNA BLOOD-EDTA WILD BOAR 00969/16 cAsE 85 00969 Narewka DNA SI LEEN WILD BOAR 00969/16 78 cAsE 85 00969 Narewka DNA LYMPHNODE WILD BOAR 00969/16 79 CASE 85 00969 Narewka DNA KIDNEY WILD BOAR 00969/16 80 CASE 85 00969 Narewka DNA BLOOD-EDTA WILD BOAR o45r/16 81 cAsE 87 04513 SupraSl DNA BLOOD-EDTA WILD BOAR o5778/t6 82 cAsE 89 05778 Narewka DNA BONE MARROW WILD BOAR 06985/16 ¿l5 cAsE 90 06985 Biatow¡eia DNA BONE MARROW WILD BOAR 0763LlL6 84 cAsE 91 0763L Biatowie2a DNA BONE MARROW WILD BOAR 10375/L6 85 cAsE 93 10375 Narewka DNA BONE MARROW WILD BOAR L299O/t6 86 CASE 94 L2990 Narewka DNA BONE MARROW WILD BOAR 140551t6 87 cAsE 95 14055 B¡afowie2a DNA BONE MARROW WILD BOAR r40s6/16 88 cAsE 95 14056 Biaiowieia DNA BONE MARROW DOMESTIC PIG Ls337 /L6 89 OUTBREAK 4 15337 Hajnówka DNA BLOOD-EDTA DOMESTIC PIG 15337 /16 90 OUTBREAK 4 15337 Hainówka DNA KIDNEY DOMESTIC PIG rs337 /L6 91 OUTBREAK 4 15337 Hainówka DNA SPLEEN DOMESTIC PIG 15338/16 92 OUTBREAK 4 15338 Hainówka DNA BLOOD-EDTA DOMESTIC PIG 1s338/16 93 OUTBREAK 4 15338 Hainówka DNA KIDNEY DOMESTIC PIG 1s338/16 94 OUTBREAK 4 15338 Hajnówka DNA SPLEEN DOMESTIC PIG 15339/16 >f, OUTBREAK 4 1s339 Hainówka DNA BLOOD-EDTA DOMESTIC PIG L5339116 96 OUTBREAK 4 15339 Hainówka DNA K DNEY DOMESTIC PIG Ls339/t6 97 OUTBREAK 4 15339 Hainówka DNA SPLEEN DOMESTIC PIG r$40/L6 98 OUTBREAK 4 15340 Hainówka DNA BLOOD.EDTA DOMESTIC PIG Ls34O/16 99 OUTBREAK 4 15340 Hainówka DNA KIDNEY DOMESTIC PIG Ls34O/L6 100 OUTBREAK 4 15340 Hainówka DNA SPLEEN DOMESTIC PIG L534Ut6 101 OUTBREAK 4 15341 Hainówka DNA BLOOD-EDTA DOMESTIC PIG r534t/16 LO2 OUTBREAK 4 15341 Hajnówka DNA KIDNEY DOMESTIC PIG r534rl16 103 OUTBREAK 4 15341 Hainówka DNA SPLEEN DOMESTIC PIG L5553/L6 104 OUTBREAK 4 15553 Hainówka DNA KIDNEY DOMESTIC PIG 15553/16 105 OUTBREAK 4 15553 Hainówka DNA SPLEEN DOMESTIC PIG L5554/L6 106 OUTBREAK 4 15554 Hainówka DNA KIDNEY DOMESTIC PIG tsss4/L6 LO7 OUTBREAK 4 15554 Hainówka DNA SPLEEN DOMESTIC PIG 15555/16 108 OUTBREAK 4 1s555 Hainówka DNA KIDNEY DOMESTIC PIG 15555/16 109 OUTBREAK 4 15555 Hajnówka DNA SPLEEN DOMESTIC PIG 15556/16 110 OUTBREAK 4 1f,f,f,o Hainówka DNA K DNEY DOMESTIC PIG 1s556/16 111 OUTBREAK 4 15556 Hainówka DNA SPLEEN WILD BOAR 15777 lL6 LL2 cAsE 96 r5777 Czv2e DNA BONE MARROW WILD BOAR L5778/L6 LL3 cAsE 96 L5778 Czy2e DNA BONE MARROW WILD BOAR 15779/76 tL4 CASE 96 15779 Czv2e DNA BONE MARROW WILD BOAR r5780/16 115 cAsE 96 15780 DNA BONE MARROW WILD BOAR L57801t6 116 CASE 96 15780 Czv2e DNA SPLEEN WILD BOAR 16080/16 tt7 cAsE 98 16080 Biafowie¿a DNA BONE MARROW WILD BOAR !6s54/t6 118 cAsE 99 16554 Hainówka DNA BONE MARROW WILD BOAR 16555/16 119 cAsE 99 165s5 Hainówka DNA SPLEEN Ctra.