Supplement Table 5. Significantly altered proteins with Aquamin® (p value <0.05 across all conditions and subjects) Aquamin Proteins Gene 1.5mM 2.1mM 3.0mM 4.5mM Cadherin-17 CDH17 2.834±0.216 2.952±0.600 3.235±0.672 3.616±0.703 Zinc transporter ZIP4 SLC39A4 2.012±0.545 2.077±0.457 2.628±0.769 2.833±0.958 Calcium-activated chloride channel regulator 4 CLCA4 2.029±0.281 2.236±0.506 2.311±0.269 3.006±0.742 Natural resistance-associated macrophage protein 2 SLC11A2 1.911±0.419 2.203±0.427 2.564±0.357 2.683±0.543 Desmoglein-2 DSG2 2.069±0.172 2.104±0.252 2.283±0.367 2.356±0.396 Ly6/PLAUR domain-containing protein 8 LYPD8 2.117±0.215 2.137±0.502 2.459±0.396 2.642±0.927 Sterol 26-hydroxylase, mitochondrial CYP27A1 1.791±0.351 2.018±0.308 2.156±0.416 2.233±0.574 Chloride anion exchanger SLC26A3 1.773±0.291 1.911±0.426 1.889±0.284 2.098±0.385 Carcinoembryonic antigen-related cell adhesion molecule 7 CEACAM7 1.835±0.322 1.998±0.077 2.227±0.372 2.438±0.503 Sodium/glucose cotransporter 1 SLC5A1 1.398±0.161 1.726±0.098 1.940±0.375 2.204±0.283 Tissue alpha-L-fucosidase FUCA1 1.617±0.201 1.812±0.125 1.902±0.270 2.065±0.379 Aminopeptidase N ANPEP 1.909±0.213 2.010±0.247 1.854±0.271 2.002±0.361 Inter-alpha-trypsin inhibitor heavy chain H4 ITIH4 1.456±0.076 1.776±0.310 1.791±0.320 1.977±0.195 Hydroxymethylglutaryl-CoA synthase, mitochondrial HMGCS2 1.520±0.122 1.742±0.225 1.954±0.222 2.089±0.179 Dehydrogenase/reductase SDR family member 7 DHRS7 1.407±0.176 1.569±0.184 1.683±0.225 1.838±0.316 Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 ENPP3 1.407±0.150 1.598±0.182 1.812±0.194 2.038±0.250 Transmembrane protease serine 2 TMPRSS2 1.532±0.109 1.627±0.196 1.828±0.180 2.077±0.472 Cadherin-related family member 5 CDHR5 1.507±0.143 1.613±0.148 1.768±0.230 1.949±0.478 ATP-binding cassette sub-family G member 2 ABCG2 1.558±0.140 1.761±0.089 1.781±0.142 1.804±0.219 Beta-glucuronidase GUSB 1.287±0.058 1.513±0.228 1.510±0.170 2.002±0.270 Glycogen phosphorylase, brain form PYGB 1.271±0.081 1.412±0.182 1.577±0.225 1.667±0.270 Fucose mutarotase FUOM 1.436±0.133 1.759±0.253 1.832±0.186 2.041±0.575 Solute carrier family 15 member 1 SLC15A1 1.556±0.094 1.675±0.138 1.725±0.147 1.806±0.361 Multidrug resistance protein 1 ABCB1 1.297±0.032 1.451±0.039 1.547±0.091 1.698±0.216 Junction plakoglobin JUP 1.597±0.176 1.712±0.118 1.768±0.130 1.774±0.111 P2X purinoceptor 4 P2RX4 1.411±0.135 1.516±0.167 1.567±0.130 1.630±0.267 Long-chain-fatty-acid--CoA ligase 5 ACSL5 1.369±0.155 1.451±0.165 1.582±0.202 1.649±0.297 Hephaestin HEPH 1.501±0.173 1.647±0.280 1.830±0.261 1.833±0.264 Amine oxidase [flavin-containing] A MAOA 1.344±0.135 1.457±0.156 1.475±0.174 1.580±0.262 Sulfotransferase family cytosolic 1B member 1 SULT1B1 1.533±0.184 1.585±0.199 1.680±0.124 1.736±0.367 Beta-galactosidase GLB1 1.321±0.066 1.462±0.087 1.581±0.171 1.619±0.180 Peroxiredoxin-like 2A FAM213A 1.296±0.062 1.469±0.142 1.527±0.138 1.597±0.184 Beta-hexosaminidase subunit beta HEXB 1.296±0.027 1.365±0.066 1.486±0.129 1.554±0.169 Peroxisomal acyl-coenzyme A oxidase 2 ACOX2 1.316±0.037 1.418±0.092 1.490±0.128 1.564±0.188 Guanine nucleotide-binding protein subunit alpha-11 GNA11 1.302±0.067 1.439±0.150 1.562±0.251 1.57±0.210 Superoxide dismutase, mitochondrial SOD2 1.272±0.106 1.427±0.141 1.418±0.065 1.556±0.007 Unconventional myosin-VIIb MYO7B 1.259±0.024 1.371±0.100 1.465±0.133 1.476±0.158 Gelsolin GSN 1.218±0.065 1.231±0.072 1.360±0.088 1.478±0.175 Ectonucleoside triphosphate diphosphohydrolase 8 ENTPD8 1.489±0.061 1.455±0.119 1.509±0.076 1.608±0.144 Inositol 1,4,5-trisphosphate receptor type 3 ITPR3 1.244±0.055 1.359±0.124 1.442±0.121 1.450±0.130 Diphosphoinositol polyphosphate phosphohydrolase 2 NUDT4 1.216±0.057 1.301±0.092 1.437±0.043 1.587±0.208 Ubiquitin-like protein 3 UBL3 1.320±0.056 1.456±0.078 1.491±0.076 1.502±0.182 Plectin PLEC 1.273±0.096 1.454±0.077 1.434±0.142 1.506±0.027 Serotransferrin TF 1.375±0.034 1.442±0.160 1.497±0.219 1.568±0.271 Peroxisomal acyl-coenzyme A oxidase 1 ACOX1 1.252±0.093 1.243±0.091 1.373±0.082 1.457±0.081 Lysosomal alpha-glucosidase GAA 1.256±0.035 1.314±0.036 1.460±0.029 1.492±0.043 Keratin, type II cytoskeletal 8 KRT8 1.250±0.098 1.524±0.092 1.433±0.037 1.488±0.103 Very-long-chain 3-oxoacyl-CoA reductase HSD17B12 1.284±0.014 1.418±0.135 1.532±0.110 1.533±0.142 Alpha-methylacyl-CoA racemase AMACR 1.269±0.050 1.439±0.024 1.428±0.032 1.419±0.076 Ectonucleoside triphosphate diphosphohydrolase 5 ENTPD5 1.257±0.095 1.336±0.041 1.342±0.092 1.387±0.153 Desmocollin-2 DSC2 1.386±0.072 1.345±0.109 1.385±0.134 1.427±0.123 Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 PLOD2 1.186±0.070 1.288±0.125 1.406±0.055 1.429±0.062 Cytosolic 10-formyltetrahydrofolate dehydrogenase ALDH1L1 1.143±0.054 1.210±0.051 1.318±0.088 1.376±0.127 Pituitary tumor-transforming gene 1 protein PTTG1IP 1.325±0.069 1.225±0.052 1.375±0.022 1.399±0.166 Medium-chain specific acyl-CoA dehydrogenase, mitochondrial ACADM 1.164±0.058 1.309±0.039 1.323±0.066 1.362±0.136 UDP-glucuronosyltransferase 1-10 UGT1A10 1.298±0.131 1.327±0.143 1.388±0.033 1.394±0.138 Chloride intracellular channel protein 3 CLIC3 1.373±0.143 1.328±0.135 1.504±0.199 1.500±0.240 Major vault protein MVP 1.205±0.087 1.283±0.093 1.319±0.086 1.393±0.169 Lysosome membrane protein 2 SCARB2 1.261±0.084 1.295±0.123 1.366±0.149 1.384±0.120 Carnitine O-palmitoyltransferase 1, liver isoform CPT1A 1.196±0.078 1.278±0.062 1.353±0.102 1.360±0.145 Calcium-binding mitochondrial carrier protein Aralar2 SLC25A13 1.152±0.058 1.288±0.093 1.320±0.110 1.380±0.133 Acid sphingomyelinase-like phosphodiesterase 3a SMPDL3A 1.411±0.102 1.356±0.137 1.356±0.014 1.327±0.130 Acyl-CoA:lysophosphatidylglycerol acyltransferase 1 LPGAT1 1.174±0.045 1.263±0.090 1.304±0.022 1.331±0.105 Xanthine dehydrogenase/oxidase XDH 1.267±0.039 1.345±0.082 1.384±0.052 1.403±0.059 Phytanoyl-CoA dioxygenase, peroxisomal PHYH 1.119±0.025 1.275±0.115 1.321±0.068 1.357±0.127 Sigma intracellular receptor 2 TMEM97 1.304±0.120 1.484±0.123 1.396±0.090 1.325±0.124 Very long-chain specific acyl-CoA dehydrogenase, mitochondrial ACADVL 1.143±0.050 1.326±0.046 1.363±0.052 1.375±0.051 Phosphatidate cytidylyltransferase 1 CDS1 1.126±0.014 1.283±0.121 1.271±0.090 1.309±0.091 Pyruvate carboxylase, mitochondrial PC 1.290±0.031 1.331±0.155 1.360±0.162 1.345±0.134 Unconventional myosin-XVB MYO15B 1.257±0.093 1.246±0.068 1.322±0.011 1.344±0.133 Protein phosphatase 1 regulatory subunit 3G PPP1R3G 1.239±0.035 1.265±0.046 1.394±0.187 1.325±0.114 DnaJ homolog subfamily A member 4 DNAJA4 1.254±0.107 1.212±0.066 1.336±0.041 1.401±0.138 Procollagen lysine hydroxylase and glycosyltransferase LH3 PLOD3 1.147±0.047 1.193±0.047 1.294±0.097 1.334±0.111 Thiosulfate sulfurtransferase TST 1.209±0.013 1.273±0.111 1.357±0.112 1.420±0.203 ATP-dependent 6-phosphofructokinase, liver type PFKL 1.143±0.044 1.193±0.082 1.278±0.110 1.276±0.083 Polypeptide N-acetylgalactosaminyltransferase 12 GALNT12 1.196±0.060 1.266±0.089 1.297±0.089 1.287±0.112 Carboxylesterase 3 CES3 1.240±0.101 1.313±0.043 1.245±0.030 1.311±0.120 Serine beta-lactamase-like protein LACTB, mitochondrial LACTB 1.176±0.049 1.307±0.105 1.288±0.034 1.313±0.026 Basement membrane-specific heparan sulfate proteoglycan protein HSPG2 1.131±0.013 1.227±0.040 1.237±0.050 1.316±0.104 Maestro heat-like repeat-containing protein family member 1 MROH1 1.135±0.027 1.315±0.022 1.354±0.060 1.331±0.092 ATP-binding cassette sub-family D member 3 ABCD3 1.176±0.046 1.306±0.138 1.357±0.095 1.307±0.076 Mitochondrial proton/calcium exchanger protein LETM1 1.140±0.050 1.173±0.069 1.211±0.085 1.251±0.097 Aldose 1-epimerase GALM 1.157±0.052 1.169±0.046 1.262±0.084 1.294±0.049 Aldehyde dehydrogenase, mitochondrial ALDH2 1.167±0.031 1.240±0.016 1.252±0.037 1.282±0.063 IST1 homolog IST1 1.187±0.004 1.127±0.030 1.243±0.035 1.261±0.115 Acyl-coenzyme A thioesterase 1 ACOT1 1.162±0.037 1.239±0.022 1.248±0.012 1.238±0.047 2,4-dienoyl-CoA reductase, mitochondrial DECR1 1.120±0.029 1.127±0.017 1.177±0.008 1.216±0.079 Serpin H1 SERPINH1 1.132±0.028 1.192±0.027 1.192±0.061 1.228±0.042 Protein mono-ADP-ribosyltransferase PARP4 PARP4 1.173±0.024 1.178±0.014 1.235±0.047 1.273±0.104 Peroxisomal acyl-coenzyme A oxidase 3 ACOX3 1.168±0.049 1.193±0.019 1.247±0.038 1.206±0.024 StAR-related lipid transfer protein 5 STARD5 1.204±0.065 1.221±0.091 1.297±0.089 1.221±0.094 MAGUK p55 subfamily member 7 MPP7 1.052±0.017 1.146±0.047 1.108±0.018 1.183±0.052 Methylglutaconyl-CoA hydratase, mitochondrial AUH 1.162±0.038 1.246±0.006 1.282±0.062 1.241±0.092 Farnesyl pyrophosphate synthase FDPS 1.137±0.013 1.242±0.088 1.241±0.068 1.229±0.087 Catechol O-methyltransferase domain-containing protein 1 COMTD1 1.208±0.026 1.265±0.063 1.216±0.036 1.215±0.068 Glycogen debranching enzyme AGL 1.088±0.026 1.121±0.045 1.137±0.035 1.171±0.033 Succinate dehydrogenase [ubiquinone] flavoprotein, mitochondrial SDHA 1.073±0.003 1.124±0.051 1.171±0.042 1.176±0.066 Neuroplastin NPTN 1.155±0.013 1.140±0.012 1.144±0.008 1.181±0.065 Vinculin VCL 1.095±0.015 1.237±0.065 1.178±0.060 1.188±0.049 Villin-1 VIL1 1.092±0.025 1.123±0.035 1.142±0.014 1.144±0.051 Disintegrin and metalloproteinase domain-containing protein 9 ADAM9 0.903±0.036 0.872±0.020 0.916±0.007 0.899±0.028 Aspartate--tRNA ligase, mitochondrial DARS2 0.949±0.017 0.906±0.011 0.880±0.018 0.875±0.029 E3 ubiquitin-protein ligase HUWE1 HUWE1 0.919±0.030 0.900±0.026 0.903±0.019 0.862±0.044 Integrin alpha-3 ITGA3 0.919±0.027 0.903±0.028 0.859±0.010 0.868±0.035 Talin-1 TLN1 0.919±0.012 0.892±0.016 0.881±0.020 0.867±0.050 Nuclear pore complex protein Nup155 NUP155 0.832±0.011