BioServe Space Technologies Space Life Sciences Hardware and Expertise Chips in Space Informational Webinar December 14, 2017 Speaker: Louis Stodieck PhD Director [email protected] 303-492-4010 Our Mission: Collaborate with industry, academia and government to conduct high impact, high quality space life sciences research More Information: www.colorado.edu/engineering/BioServe/ n Based at University of Colorado n Operating since 1987 n First space flight experiment flown in 1991 STS-37 n Faculty, staff and students with extensive space life sciences and supporting space flight hardware design expertise BioServe Space Turn Key, End to End Space Flight Services n PI Team Science Support, Technical n Extensive, Off-the-shelf Flight Guidance Hardware Solutions n Feasibility / Trade Study Assistance n Flight and Ground Safety n Science Team Hardware Training Processing n Payload Manifest Negotiations n In-house Manufacturing, Assembly and Test n Payload Mission Management n Flight Qualification Services n Engineering Integration – Understand and Satisfy All NASA n Launch and Landing site support Documentation Requirements n Physical Payload Integration and n Ground Test Facilities/support De-integration – Flight Readiness n Mission Simulations and n Crew Procedure Writing and Crew Experiment Verification Testing training n Software / Firmware Development n Operations Planning n Real Time Operations n Closely allied with Industry and Government, especially NASA n Dedicated 24/7 Remote Payload Operations Control Center n International Experience n Real-time Problem Solving BioServe Flight History n BioServe supported life science experiments on over 55 space flight missions n Russian Mir Space Station n 39 Shuttle missions n Soyuz/Progress/HTV/SpaceX/ Orbital ATK n International Space Station Since 2001 n Hundreds of individual investigations n Molecular, cellular, small organism, plant and animal studies Areas of Research Expertise n Muscle atrophy and bone loss n Mammalian cell and tissue culture n Microbial studies– virulence and antibiotic effectiveness, secondary compound production n Small organisms n Model organisms – bacteria, yeast, fruit flies, nematodes n Rodent Research – animal disease models n Supported most areas of life science research Results oriented n Over 350 refereed journal articles published as a result of BioServe support of life science research n High quality science return is our philosophy n We work hand in hand with our science teams to make the science a success and ensure it is conducted in space as close as possible to how it is done in the lab on Earth n Support both flight and ground controls, including microgravity simulations where beneficial n Ensure results are due to microgravity exposure rather than a result of hardware limitations or other challenges associated with conducting science in space. Examples of Research Recently/Currently Supporting n Cell and Stem Cell Research n Osteomics – Gravitational Regulation of Osteoblast Genomics and Metabolism - an NIH sponsored investigation (SpaceX-9) n Patient-specific cardiomyocytes produced from skin cells to examine cardiac function, structure and gene expression in space (SpaceX-9) n Expansion of human mesenchymal SCs and induced pluripotent SCs (SpaceX-10) n Cardiovascular progenitor cells (neonatal and adult). Examined expression of microRNAs and genes related to mechanotransduction, cardiogenesis, cell cycling, DNA repair and paracrine signaling (SpaceX-11) n Lung Tissue - Examined the effects of microgravity and radiation on stem and progenitor cells grown on a novel scaffold of human acellularized lung tissue treated with nanoparticles containing critical growth factors. (SpaceX-12) n Synthetic Bone – Will examine Tetranite™ (TN), a bone adhesive developed by LaunchPad Medical (LPM). (SpaceX-13) n 3D Cell Culture Research n Magnetic system for 3d cell culturing in space (OA7) n 3D Spheroids for chemotherapeutic screening (OA7) n NIH NCATS – Kidney Cells (Nortis Chip) n Research group from the University of Washington n Developing continuous perfusion system for the Nortis Chip Current NIH NCATS Organ on a Chip Project • Nortis Triplex Chip • Controlled 37ºC and 5%CO2 during transportation and while on orbit • Continuous perfusion 750 ul per day • Ability to change out media cassettes • Ability to change out fixation/preservation cassettes MPS Perfusion System integrated • Entire chip module can be frozen into BioServe’s SABL unit • Integrated into BioServe’s SABL unit Hardware Expertise n Extensive history designing, developing cell culture systems and hardware n Temperature and Atmosphere Control n SABL (Space Automated Bioproduct Lab) n Two units operating on ISS now n Precise temperature control incubator (-5°C to 43°C) n Atmosphere composition control (e.g. 5%CO2) n Each unit has over 23 liters of volume for scientific hardware n Has 2 USB ports to support powered USB devices n Has 2 Ethernet LAN connections available n Has switchable 28vdc and 5vdc power supplies dedicated to experiment use n CGBA n Two units on board ISS since 2001 n -16°C to 37°C n Can support powered experiments n Can be used for transportation to/from ISS for temperature control and experiment control Hardware Cont. n BioCells n Supports suspension or adherent cell culture (6 well or single-well) n Gas exchange provided passively through membrane surfaces n Inlet, outlet and sampling ports for fluid exchanges n 12-well plate – in development Hardware Continued n Microscope n Standard Phase Contrast Inverted Microscope with 40X, 20X, 10X and 4X objectives. n Easy to use with on board camera system for still and video imagery Other Relevant Hardware Expertise n MOBIAS (Multiple On-orbit Bioreactors with Instrumentation and Automated Sampling n Perfusion approach with automated sampling n SLCC (Single-Loop Cell Culture) system n Self contained system for perfusion and sampling with media and gas exchanger n Chab (Culture Habitat) n Passive gas exchange, sampling and inoculation Other relevant expertise n Microfluidics n Pumps – syringe, peristaltic, micropumps etc. n Sterile technique – lab and loading hardware n Biocompatability n Launching live or frozen with activation on orbit using thawing system n Fluid exhanges including media, preservation, fixation n Access to -80C assets on orbit for maintenance of samples until return to space BioServe as Turnkey Solution n Highly experienced team n Large inventory of available flight hardware n Can modify or design new hardware, as needed n Full knowledge of all details required to successfully fly life science in space n Allow PI team to focus on science, not on flight process requirements n BioServe team is dedicated to success in space! Thank you!.