10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 DDX3 / DDX3X Antibody (N-Terminus) Rabbit Polyclonal Antibody Catalog # ALS12227 Specification DDX3 / DDX3X Antibody (N-Terminus) - Product Information Application ICC, IF, WB Primary Accession O00571 Reactivity Human, Mouse Host Rabbit Clonality Polyclonal Calculated MW 73kDa KDa DDX3 / DDX3X Antibody (N-Terminus) - Additional Information Gene ID 1654 Other Names Immunocytochemistry of DDX3 in HepG2 ATP-dependent RNA helicase DDX3X, cells with DDX3 antibody at 10 ug/ml. 3.6.4.13, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, Helicase-like protein 2, HLP2, DDX3X, DBX, DDX3 Target/Specificity 16 amino acid peptide from near the amino terminus of human DDX3. Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. Precautions DDX3 / DDX3X Antibody (N-Terminus) is for Immunofluorescence of DDX3 in HepG2 cells research use only and not for use in with DDX3 antibody at 20 ug/ml. diagnostic or therapeutic procedures. DDX3 / DDX3X Antibody (N-Terminus) - Protein Information Name DDX3X Synonyms DBX {ECO:0000303|PubMed:15294876}, DDX3 Function Multifunctional ATP-dependent RNA helicase Page 1/8 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 (PubMed:<a href="http://www.uniprot.org/c itations/17357160" target="_blank">17357160</a>, PubMed:<a href="http://www.uniprot.org/ci tations/21589879" target="_blank">21589879</a>, PubMed:<a href="http://www.uniprot.org/ci tations/31575075" target="_blank">31575075</a>). The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity (PubMed:<a href="http://www.uniprot.org/c itations/29222110" target="_blank">29222110</a>). In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs (PubMed:<a href="http://w Western blot of DDX3 in HepG2 cell lysate ww.uniprot.org/citations/17357160" target="_blank">17357160</a>, with DDX3 antibody at (A) 0.5 and (B) 1 PubMed:<a href="http://www.uniprot.org/ci ug/ml. tations/21589879" target="_blank">21589879</a>). Binds RNA G- quadruplex (rG4s) structures, DDX3 / DDX3X Antibody (N-Terminus) - including those located in the 5'-UTR of Background NRAS mRNA (PubMed:<a href="http://www. uniprot.org/citations/30256975" Multifunctional ATP-dependent RNA helicase. target="_blank">30256975</a>). Involved The ATPase activity can be stimulated by in many cellular processes, which do not various ribo- and deoxynucleic acids indicative necessarily require its ATPase/helicase for a relaxed substrate specificity. In vitro can catalytic activities (Probable). Involved in unwind partially double-stranded DNA with a transcription regulation (PubMed:<a href=" preference for 5'- single-stranded DNA http://www.uniprot.org/citations/16818630" overhangs. Is involved in several steps of gene target="_blank">16818630</a>, expression, such as transcription, mRNA PubMed:<a href="http://www.uniprot.org/ci maturation, mRNA export and translation. tations/18264132" However, the exact mechanisms are not target="_blank">18264132</a>). known and some functions may be specific for Positively regulates CDKN1A/WAF1/CIP1 a subset of mRNAs. Involved in transcriptional transcription in an SP1-dependent manner, regulation. Can enhance transcription from the hence inhibits cell growth. This function CDKN1A/WAF1 promoter in a SP1-dependent requires its ATPase, but not helicase activity manner. Found associated with the E-cadherin (PubMed:<a href="http://www.uniprot.org/c promoter and can down-regulate transcription itations/16818630" from the promoter. Involved in regulation of target="_blank">16818630</a>, translation initiation. Proposed to be involved PubMed:<a href="http://www.uniprot.org/ci in positive regulation of translation such as of tations/18264132" cyclin E1/CCNE1 mRNA and specifically of target="_blank">18264132</a>). CDKN1A up-regulation may be cell-type specific mRNAs containing complex secondary (PubMed:<a href="http://www.uniprot.org/c structures in their 5'UTRs; these functions itations/18264132" seem to require RNA helicase activity. target="_blank">18264132</a>). Binds Specifically promotes translation of a subset of CDH1/E-cadherin promoter and represses viral and cellular mRNAs carrying a 5'proximal its transcription (PubMed:<a href="http://w stem-loop structure in their 5'UTRs and ww.uniprot.org/citations/18264132" cooperates with the eIF4F complex. Proposed target="_blank">18264132</a>). to act prior to 43S ribosomal scanning and to Potentiates HNF4A-mediated MTTP locally destabilize these RNA structures to transcriptional activation; this function allow recognition of the mRNA cap or loading Page 2/8 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 requires ATPase, but not helicase activity. onto the 40S subunit. After association with Facilitates HNF4A acetylation, possibly 40S ribosomal subunits seems to be involved catalyzed by CREBBP/EP300, thereby in the functional assembly of 80S ribosomes; increasing the DNA-binding affinity of HNF4 the function seems to cover translation of to its response element. In addition, mRNAs with structured and non-structured disrupts the interaction between HNF4 and 5'UTRs and is independent of RNA helicase SHP that forms inactive heterodimers and activity. Also proposed to inhibit enhances the formation of active HNF4 cap-dependent translation by competetive homodimers. By promoting HNF4A-induced interaction with EIF4E which can block the MTTP expression, may play a role in lipid EIF4E:EIF4G complex formation. Proposed to homeostasis (PubMed:<a href="http://www. be involved in stress response and stress uniprot.org/citations/28128295" granule assembly; the function is independent target="_blank">28128295</a>). May of RNA helicase activity and seems to involve positively regulate TP53 transcription association with EIF4E. May be involved in (PubMed:<a href="http://www.uniprot.org/c itations/28842590" nuclear export of specific mRNAs but not in target="_blank">28842590</a>). bulk mRNA export via interactions with XPO1 Associates with mRNPs, predominantly with and NXF1. Also associates with polyadenylated spliced mRNAs carrying an exon junction mRNAs independently of NXF1. Associates with complex (EJC) (PubMed:<a href="http://ww spliced mRNAs in an exon junction complex w.uniprot.org/citations/17095540" (EJC)-dependent manner and seems not to be target="_blank">17095540</a>, directly involved in splicing. May be involved in PubMed:<a href="http://www.uniprot.org/ci nuclear mRNA export by association with DDX5 tations/18596238" and regulating its nuclear location. Involved in target="_blank">18596238</a>). Involved innate immune signaling promoting the in the regulation of translation initiation production of type I interferon (IFN-alpha and (PubMed:<a href="http://www.uniprot.org/c IFN-beta); proposed to act as viral RNA sensor, itations/18628297" signaling intermediate and transcriptional target="_blank">18628297</a>, coactivator. Involved in TBK1 and PubMed:<a href="http://www.uniprot.org/ci IKBKE-dependent IRF3 activation leading to tations/17667941" IFNB induction, plays a role of scaffolding target="_blank">17667941</a>, adapter that links IKBKE and IRF3 and PubMed:<a href="http://www.uniprot.org/ci coordinates their activation. Also found tations/22872150" associated with IFNB promoters; the function is target="_blank">22872150</a>). Not independent of IRF3. Can bind to viral RNAs involved in the general process of and via association with MAVS/IPS1 and translation, but promotes efficient DDX58/RIG-I is thought to induce signaling in translation of selected complex mRNAs, early stages of infection. Involved in regulation containing highly structured 5'-untranslated of apoptosis. May be required for activation of regions (UTR) (PubMed:<a href="http://ww w.uniprot.org/citations/20837705" the intrinsic but inhibit activation of the target="_blank">20837705</a>, extrinsic apoptotic pathway. Acts as an PubMed:<a href="http://www.uniprot.org/ci antiapoptotic protein through association with tations/22872150" GSK3A/B and BIRC2 in an apoptosis target="_blank">22872150</a>). This antagonizing signaling complex; activation of function depends on helicase activity death receptors promotes caspase-dependent (PubMed:<a href="http://www.uniprot.org/c cleavage of BIRC2 and DDX3X and relieves the itations/20837705" inhibition. May be involved in mitotic target="_blank">20837705</a>, chromosome segregation. Appears to be a PubMed:<a href="http://www.uniprot.org/ci prime target for viral manipulations. Hepatitis tations/22872150" B virus (HBV) polymerase and possibly vaccinia target="_blank">22872150</a>). Might virus (VACV) protein K7 inhibit IFNB induction facilitate translation by resolving secondary probably by dissociating DDX3X from TBK1 or structures of 5'-UTRs during ribosome IKBKE. Is involved in hepatitis C virus (HCV) scanning (PubMed:<a href="http://www.uni replication; the function may involve the prot.org/citations/20837705" association with HCV core protein. HCV core target="_blank">20837705</a>). protein inhibits the IPS1-dependent function in Page 3/8 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Alternatively, may act prior to 43S viral RNA sensing and may switch the function ribosomal scanning and promote 43S from a INFB inducing to a HCV replication pre-initiation