IOBC / WPRS Working group “Integrated Protection of Olive Crops” OILB / SROP Groupe de travail “Protection Intégrée des Olivaies” Proceedings of the meeting Comptes rendus de la réunion at / à Florence (Italy) 26-28 October 2005 Edited by: Argyro Kalaitzaki IOBC wprs Bulletin Bulletin OILB srop Vol. 30 (9), 2007 The content of the contributions is in the responsibility of the authors The IOBC/WPRS Bulletin is published by the International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palearctic Regional Section (IOBC/WPRS) Le Bulletin OILB/SROP est publié par l‘Organisation Internationale de Lutte Biologique et Intégrée contre les Animaux et les Plantes Nuisibles, section Regionale Ouest Paléarctique (OILB/SROP) Copyright: IOBC/WPRS 2007 The Publication Commission of the IOBC/WPRS: Horst Bathon Luc Tirry Federal Biological Research Center University of Gent for Agriculture and Forestry (BBA) Laboratory of Agrozoology Institute for Biological Control Department of Crop Protection Heinrichstr. 243 Coupure Links 653 D-64287 Darmstadt (Germany) B-9000 Gent (Belgium) Tel +49 6151 407-225, Fax +49 6151 407-290 Tel +32-9-2646152, Fax +32-9-2646239 e-mail: [email protected] e-mail: [email protected] Address General Secretariat: Dr. Philippe C. Nicot INRA – Unité de Pathologie Végétale Domaine St Maurice - B.P. 94 F-84143 Monfavet cedex France ISBN 92-9067-205-4 http://www.iobc-wprs.org i Preface This bulletin contains the proceedings of the European meeting of the IOBC/WPRS Working Group “Integrated Protection of Olive Crops” that was held in Florence, Italy, October 26-28 2005 in the Polo Scientifico of Sesto Fiorentino. This is the second meeting of the new period of activity of the group which was started in 2003 in Chania. Approximately, 86 scientists from 12 different olive growing countries (Italy, Spain, Portugal, Greece, UK, Austria, USA, Montenrgro, Slovakia, Egypt, Tunisia, Iran) attended the meeting. During the meeting, three plenary lectures, 30 oral contributions and 40 posters were presented. Topics in this Bulletin include: Bactrocera oleae (behaviour, chemical ecology, monitoring, population dynamics, chemical, biological and biotechnical control methods, side effects), other pests of olive groves and olive agroecosystem aspects, diseases and advanced IPM strategies in olive groves. On behalf of the working group and all attendances I would like to express my gratitude to the Local Organizing Committee: Prof. Antonio Belcari (Chairperson), Dr. Marzia Cristiana Rosi, Dr. Patrizia Sacchetti and Prof. Giuseppe Surico from University of Florence, Dr. Bruno Bagnoli from Istituto Sperimentale per la Zoologia agraria of Florence, Dr. Ruggero Petacchi from Biolabs, Sant’ Anna School of Advanced Studies of Pisa and Prof. Alfio Raspi from University of Pisa, for organizing this meeting. I would like also to thank the public and private sponsors who supported financially this working group meeting and contributed substantially to its success. I believe that this Bulletin will be a useful tool for scientists and technicians who have as a target the development of integrated control strategies for pests in olive groves, the preservation of the complex of natural enemies and the reduction of pesticides inputs. Argyro Kalaitzaki Convener of the Working Group Integrated Protection of Olive Crops ii iii Contents Preface ....................................................................................................................................... i Contents ................................................................................................................................... iii List of participants ................................................................................................................... ix Opening Session The olive growing and olive oil sector in Tuscany L. Zoppi ........................................................................................................................... 1 Arsia activities in the field of olive crop protection M. Ricciolini & M. Toma ................................................................................................ 5 Bactrocera oleae: Behaviour, Chemical Ecology, Monitoring, Population Dynamics Chemical ecology of bacterial relationships with fruit flies David C. Robacker .......................................................................................................... 9 Effect of age and mating status on the antennal sensitivity of Bactrocera oleae (Rossi) (Diptera Tephritidae) male and female. A. De Cristofaro, G. Rotundo, A. Belcari, G.S. Germinara .......................................... 23 The relationship between olive fly adults and epiphytic bacteria of the olive tree Granchietti A., A. Camèra, S. Landini, M.C. Rosi, M. Librandi, P. Sacchetti, G. Marchi, G. Surico, A. Belcari.................................................................................... 25 Field assessment of different combinations of ammonia-based attractants and a synthetic female sex pheromone for the monitoring and control of the olive fruit fly, Bactrocera oleae Gmel. (Diptera: Tephritidae) in Apulia, southern Italy A. De Cristofaro, M. Cristofaro, F. Tenaglia, A. Fenio, C. Tronci .............................. 31 Inhibitory effect of water assumption on attraction to ammonia, protein baits and bacteria in Bactrocera oleae (Gmelin) Vincenzo Girolami, Alessia Piscedda, Damiano Emer, Andrea Di Bernardo, Luca Mazzon & Rita Signorini ............................................................................................... 33 Attractiveness to the olive fly of Pseudomonas putida isolated from the foregut of Bactrocera oleae Patrizia Sacchetti, Serena Landini, Aurelio Granchietti, Alessandra Camèra, Marzia Cristiana Rosi, Antonio Belcari ........................................................................ 37 Preliminary notes on the gall midges (Diptera: Cecidomyiidae) associated with the olive fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) Raffaele Sasso Gennaro Viggiani ................................................................................. 43 Augmentative releases of Eupelmus urozonus Dalm. against the olive fruit fly and observations on its facultative hyperparasitism G. Delrio, A. Lentini, A. Satta ....................................................................................... 47 On the use of the exotic oo-pupal parasitoid Fopius arisanus for the biological control of Bactrocera oleae in Italy Riccardo Moretti, Elena Lampazzi, Placido Reina and Maurizio Calvitti ................... 49 iv Presence of a symbiotic bacterium in the olive fly Bactrocera oleae (Gmelin) Vincenzo Girolami, Andrea Squartini, Luca Mazzon, Alessia Piscedda, Caterina Capuzzo ......................................................................................................................... 61 Histopathological observations in the midgut and behaviour of olive fruit fly (Bactro- cera oleae Gmelin) adults treated with a strain of Bacillus thuringiensis Berliner Luca Ruiu, Gavino Delrio, Ignazio Floris, Alberto Satta, Mario Solinas .................... 67 Some biological aspects of the Bactrocera oleae (Rossi) rearing Angelo Canale, Roberto Canovai, Augusto Loni, Alfio Raspi ...................................... 73 Bait stations field test for Bactrocera oleae (Gmelin) in the Balearic islands (Spain) M.A. Miranda, E. Martinez, M. Monerris, A. Alemany ................................................. 77 Molecular markers as useful tools for population genetics of the olive fly, Bactrocera oleae D. Segura, C. Callejas, M. D. Ochando ........................................................................ 79 Susceptibility to Bactrocera oleae (Gmelin) of some Sicilian olive cultivars Roberto Rizzo, Virgilio Caleca ...................................................................................... 89 Behavioural responses of the olive fly, Bactrocera oleae, to chemicals produced by Pseudomonas putida in laboratory bioassays Serena Landini, Aurelio Granchietti, Michele Librandi, Alessandra Camèra, Marzia Cristiana Rosi, Patrizia Sacchetti, Antonio Belcari ....................................... 101 Bactrocera oleae: Chemical, Biological and Biotechnical Control Methods, Side Effects Sterile insect technique (SIT) – an environmentally friendly approach to controlling major fruit-fly pests M. Kozαnek, C. Caceres .............................................................................................. 109 Tests on the effectiveness of kaolin and copper hydroxide in the control of Bactrocera oleae (Gmelin) Virgilio Caleca, Roberto Rizzo .................................................................................... 111 Resistance to organophosphates in Bactrocera oleae in Grecee and Cyprus John Tsitsipis, John T. Margaritopoulos, Panagiotis Skouras, Konstantinos Mathiopoulos and Nikos Serafides .............................................................................. 119 A Beauveria bassiana-based bioinsecticide for the microbial control of the olive fly (Bactrocera oleae) Massimo Benuzzi, Enrico Albonetti, Fabio Fiorentini, Edith Ladurner ..................... 125 tm Bait applications effect of Spinosad Success 0.24CB (GF-120)" formulation, on Bactrocera oleae Gmel. (Dacuol), and impact on other non target organisms in olive trees P.V. Vergoulas, D. Prophetou-Athanassiadou, E. Alimi, H. Ben Salah, C. Mavrotas, C. Jousseaume, M. Miles ..........................................................................