3050 Spruce Street, St. Louis, MO 63103 USA Tel: (800) 521-8956 (314) 771-5765 Fax: (800) 325-5052 (314) 771-5757 Custom Oligonucleotide Modifications Guide These are some of the 200 modifications that Sigma offers for both DNA and RNA oligonucleotides and probes. If you do not see the mod you require, we will order it for you. 1 Table of Contents ATTACHMENT ............................................................................................................................................................... 4 AMINO MODIFIERS ........................................................................................................................................................ 4 5′-DMS(O)MT-Amino-Modifier-C6 ...................................................................................................................... 4 5′-Amino-Modifier-C3-TFA .................................................................................................................................. 5 5′-Amino-Modifier-C12 ......................................................................................................................................... 6 5′-Amino-Modifier-C6-TFA (trifluoroacetic acid protecting group on amine) ...................................................... 7 5′-Amino-dT ........................................................................................................................................................... 8 5′-Amino-Modifier-5 .............................................................................................................................................. 9 Amino-Modifier-C2-dT ........................................................................................................................................ 10 Amino-Modifier-C6-dT ........................................................................................................................................ 11 3′-Amino-Modifier-C7-CPG ................................................................................................................................ 12 3′-GLYCERYL .............................................................................................................................................................. 13 PHOSPHATE ................................................................................................................................................................. 14 5′-Phosphate and 5′-Phosphate II ......................................................................................................................... 14 3′-Phosphate ......................................................................................................................................................... 15 THIOL ......................................................................................................................................................................... 16 5'-Thiol-Modifier C6 S-S ..................................................................................................................................... 16 3'-Thiol-Modifier-C3 S-S ..................................................................................................................................... 18 BINDING ....................................................................................................................................................................... 19 BIOTIN ........................................................................................................................................................................ 19 5′-Biotin ............................................................................................................................................................... 19 Biotin .................................................................................................................................................................... 20 Biotin-dT .............................................................................................................................................................. 21 Biotin-TEG ........................................................................................................................................................... 22 3′-Biotin-TEG-CPG.............................................................................................................................................. 23 DIGOXIGENIN .............................................................................................................................................................. 24 2,4-DINITROPHENOL-TEG .......................................................................................................................................... 25 SPACERS ....................................................................................................................................................................... 26 Spacer 9 ................................................................................................................................................................ 26 Spacer 12 .............................................................................................................................................................. 26 Spacer 18 .............................................................................................................................................................. 26 Spacer C3 ............................................................................................................................................................. 26 3′-Spacer-C3-CPG ................................................................................................................................................ 28 ANALOG ....................................................................................................................................................................... 29 CHAIN TERMINATING .................................................................................................................................................. 29 3′-dA-CPG (Cordycepin)...................................................................................................................................... 29 3′-dG-CPG ............................................................................................................................................................ 29 3′-dC-CPG ............................................................................................................................................................ 30 3′-dT-CPG ............................................................................................................................................................ 30 CHEMOTHERAPEUTIC .................................................................................................................................................. 31 Cytosine arabinoside (Ara-C, Cytarabine)............................................................................................................ 31 2′,3′-Dideoxycytidine (ddC, Zalcitabine) ............................................................................................................. 33 CONTAMINATION PREVENTION .................................................................................................................................... 34 Ribo U .................................................................................................................................................................. 34 CROSSLINKING ............................................................................................................................................................ 35 8-Br-dA ................................................................................................................................................................ 35 8-Br-dG ................................................................................................................................................................ 35 5-Br-dC ................................................................................................................................................................ 36 5-Br-dU ................................................................................................................................................................ 36 5-F-dU .................................................................................................................................................................. 37 5-I-dC ................................................................................................................................................................... 37 5-I-dU ................................................................................................................................................................... 38 DAMAGE AND REPAIR ................................................................................................................................................. 39 2′-Deoxypseudouridine ........................................................................................................................................ 39 5,6-Dihydro-dT .................................................................................................................................................... 40 2 5,6-Dihydro-dU ...................................................................................................................................................