Efficient Professional Protein and Antibody Platforms NUDCD3 Antibody Basic information: Catalog No.: UPA61519 Source: Rabbit Size: 50ul/100ul Clonality: polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: affinity purified by Protein A Useful Information: WB:1:500-2000 Applications: IHC-P:1:400-800 Reactivity: Human, Mouse, Rat, Pig Specificity: This antibody recognizes NUDCD3 protein. KLH conjugated synthetic peptide derived from human NUDCD3 Immunogen: 161-260/361 NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein in- Description: termediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition. Uniprot: Q8IVD9 Human BiowMW: 41 KDa Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Sample: U937 Cell (Human) Lysate at 30 ug Pri- mary: Anti- NUDCD3 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Sample: Hl-60 Cell (Human) Lysate at 30 ug Pri- mary: Anti- NUDCD3 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded at 37 ℃ for 30min; Antibody incubation with (NUDCD3) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining. Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .