NanoDrop 2000/2000c Spectrophotometer V1.0 User Manual The information in this publication is provided for reference only. All information contained in this publication is believed to be correct and complete. Thermo Fisher Scientific shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing, performance or use of this material. All product specifications, as well as the information contained in this publication, are subject to change without notice. This publication may contain or reference information and products protected by copyrights or patents and does not convey any license under our patent rights, nor the rights of others. We do not assume any liability arising out of any infringements of patents or other rights of third parties. We make no warranty of any kind with regard to this material, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. 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NanoDrop is a trademark of Thermo Fisher Scientific. Revised 3/09 Table of Contents 1. INTRODUCTION................................................................................................................................................................ 1-1 INSTRUMENT DESCRIPTION ................................................................................................................................................... 1-1 INSTRUMENT SPECIFICATIONS............................................................................................................................................... 1-1 Patents............................................................................................................................................................................... 1-1 SAMPLE RETENTION PEDESTAL MEASUREMENTS ................................................................................................................. 1-2 Pedestal Basic Use ............................................................................................................................................................ 1-2 CUVETTE MEASUREMENTS.................................................................................................................................................... 1-3 Cuvette Basic Use ............................................................................................................................................................. 1-3 BLANKING AND ABSORBANCE CALCULATIONS..................................................................................................................... 1-4 FLUORESCENT DYES ............................................................................................................................................................. 1-5 2. SOFTWARE......................................................................................................................................................................... 2-1 COMPUTER REQUIREMENTS .................................................................................................................................................. 2-1 SOFTWARE INSTALLATION .................................................................................................................................................... 2-1 Registering Your Instrument............................................................................................................................................. 2-2 SOFTWARE FEATURES ........................................................................................................................................................... 2-2 Left Pane........................................................................................................................................................................... 2-2 Right Pane......................................................................................................................................................................... 2-5 DATA AND ACCOUNT MANAGEMENT.................................................................................................................................... 2-7 My Data ............................................................................................................................................................................ 2-7 Reports.............................................................................................................................................................................. 2-7 Autosave files.................................................................................................................................................................... 2-9 Options.............................................................................................................................................................................. 2-9 3. APPLICATIONS.................................................................................................................................................................. 3-1 OVERVIEW ............................................................................................................................................................................ 3-1 QUICK START ........................................................................................................................................................................ 3-1 MEASUREMENT RANGES ....................................................................................................................................................... 3-2 NUCLEIC ACID ...................................................................................................................................................................... 3-3 Overview........................................................................................................................................................................... 3-3 Nucleic Acid Calculations ................................................................................................................................................ 3-3 Unique Screen Features .................................................................................................................................................... 3-4 Making Nucleic Acid Measurements................................................................................................................................3-5 Oligo Calc ......................................................................................................................................................................... 3-6 MICRO ARRAY ...................................................................................................................................................................... 3-8 Overview........................................................................................................................................................................... 3-8 Dye/Chromophore Editor.................................................................................................................................................. 3-8 Unique Screen Features .................................................................................................................................................... 3-8 Making Micro Array Measurements................................................................................................................................. 3-9 Oligo Calc ....................................................................................................................................................................... 3-10 UV-VIS ............................................................................................................................................................................... 3-13 Overview......................................................................................................................................................................... 3-13 Unique Screen Features .................................................................................................................................................. 3-13 Making UV-Vis Measurements ...................................................................................................................................... 3-14 PROTEIN A280 ...................................................................................................................................................................