DDX11 Antibody Cat. No.: 55-186 DDX11 Antibody Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat- anti-rabbit secondary antibodies were used for the analysis. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse This DDX11 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 656-683 amino acids from the Central region of human DDX11. TESTED APPLICATIONS: Flow, WB For WB starting dilution is: 1:1000 APPLICATIONS: For FACS starting dilution is: 1:10~50 PREDICTED MOLECULAR 108 kDa WEIGHT: September 24, 2021 1 https://www.prosci-inc.com/ddx11-antibody-55-186.html Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: DDX11 Probable ATP-dependent RNA helicase DDX11, CHL1-related protein 1, hCHLR1, DEAD/H ALTERNATE NAMES: box protein 11, Keratinocyte growth factor-regulated gene 2 protein, KRG-2, DDX11, CHL1, CHLR1, KRG2 ACCESSION NO.: Q96FC9 PROTEIN GI NO.: 74731686 GENE ID: 1663 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their BACKGROUND: distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX11 encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. DDX11 is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. REFERENCES: 1) Leman, A.R., et al. J. Cell. Sci. 123 (PT 5), 660-670 (2010) : 2) Farina, A., et al. J. Biol. Chem. 283(30):20925-20936(2008) 3) Parish, J.L., et al. Mol. Cell 24(6):867-876(2006) September 24, 2021 2 https://www.prosci-inc.com/ddx11-antibody-55-186.html 4) Parish, J.L., et al. J. Cell. Sci. 119 (PT 23), 4857-4865 (2006) : ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 24, 2021 3 https://www.prosci-inc.com/ddx11-antibody-55-186.html.