Department of Civil & Environmental Engineering University of Massachusetts College of Engineering University of Massachusetts September 2012 CONTENTS UNIVERSITY OF MASSACHUSETTS ......................................................................................................................... 1 CONTENTS ...................................................................................................................................................................... 2 A. LABORATORY SAFETY AND SECURITY ........................................................................................................... 1 1. GENERAL RULES .......................................................................................................................................................... 1 2. EMERGENCY ................................................................................................................................................................ 2 3. DISPOSAL OF CHEMICALS ............................................................................................................................................ 2 a. What chemical wastes are hazardous or non-hazardous? ...................................................................................... 2 b. Disposal of hazardous wastes ................................................................................................................................. 2 4. FUME HOODS ............................................................................................................................................................... 3 5. SECURITY .................................................................................................................................................................... 3 B. LABORATORY MANAGEMENT ............................................................................................................................ 4 1. FACULTY LABORATORY COORDINATORS .................................................................................................................... 4 2. LABORATORY MANAGERS ........................................................................................................................................... 4 3. EQUIPMENT MANAGERS .............................................................................................................................................. 6 4. QC OFFICERS ............................................................................................................................................................... 6 5. LABORATORY USERS ................................................................................................................................................... 7 C. PERSONAL LAB SUPPLIES..................................................................................................................................... 7 D. LABWARE ................................................................................................................................................................... 8 1. NON-VOLUMETRIC GLASSWARE .................................................................................................................................. 8 2. VOLUMETRIC GLASSWARE .......................................................................................................................................... 8 3. EPPENDORF PIPETS AND AUTOMATIC PIPETERS ........................................................................................................... 8 4. REAGENT AND SAMPLE BOTTLES ................................................................................................................................ 8 5. MISCELLANEOUS LABORATORY SUPPLIES ................................................................................................................... 9 E. INSTRUMENTS .......................................................................................................................................................... 9 1. GENERAL ..................................................................................................................................................................... 9 2. ANALYTICAL BALANCES ........................................................................................................................................... 10 3. PH/ISE METERS AND ELECTRODES ........................................................................................................................... 11 4. UV-VIS SPECTROPHOTOMETERS ............................................................................................................................... 11 5. INDUCTIVELY COUPLED PLASMA (ICP) EMISSION/MS .............................................................................................. 13 6. GAS CHROMATOGRAPHS ........................................................................................................................................... 13 7. TOTAL ORGANIC CARBON (TOC) .............................................................................................................................. 14 8. TOTAL ORGANIC HALIDE (TOX) ............................................................................................................................... 15 9. LIQUID CHROMATOGRAPHS (HPLC AND IC) AND LC/MS ......................................................................................... 15 10. GAS CHROMATOGRAPH – MASS SPECTROMETER (GC/MS) ..................................................................................... 16 11. EQUIPMENT FOR MOLECULAR BIOLOGY .................................................................................................................. 16 12. AUTOCLAVES ........................................................................................................................................................... 17 13. OZONE GENERATOR ................................................................................................................................................ 18 14. TURBIDIMETERS ...................................................................................................................................................... 18 15. OTHER ..................................................................................................................................................................... 18 F. RECORD KEEPING ................................................................................................................................................. 18 1. LABORATORY NOTEBOOK ......................................................................................................................................... 18 2. LOG BOOKS ............................................................................................................................................................... 19 G. CLEANING PROCEDURES ................................................................................................................................... 19 1. GLASSWARE CLEANING ............................................................................................................................................. 19 2. LAB BENCHES ............................................................................................................................................................ 22 3. FLOORS ...................................................................................................................................................................... 23 H. LABORATORY REAGENTS .................................................................................................................................. 23 1. WATER ...................................................................................................................................................................... 23 2. CHEMICAL REAGENTS ............................................................................................................................................... 23 3. GASES ........................................................................................................................................................................ 25 I. HEATING, COOLING AND DRYING .................................................................................................................... 26 1. REFRIGERATORS & FREEZERS & ICE ......................................................................................................................... 26 2. CONSTANT TEMPERATURE CHAMBERS ...................................................................................................................... 27 3. OVENS & INCUBATORS .............................................................................................................................................. 28 4. DESICCATORS ............................................................................................................................................................ 28 J. TESTING AND ANALYSIS PROTOCOLS ............................................................................................................ 28 K. PROCUREMENT ..................................................................................................................................................... 29 For Chemical Orders ........................................................................................................................................................