Supporting information S5 - Supplementary Trees: Species-level predation network uncovers high prey specificity in a Neotropical army ant community Authors: Philipp O. Hoenle , Nico Blüthgen, Adrian Brückner, Daniel J. C. Kronauer, Brigitte Fiala, David A. Donoso, M. Alex Smith, Bryan Ospina Jara, Christoph von Beeren 1 Correspondence to: Philipp Hönle: [email protected] Christoph von Beeren: [email protected] --------------------------------------------------------------------------------------------------------------------- Content: Figure 1. Neighbor-joining tree of COI barcodes of Neotropical ants. Figure 2. RAxML tree of COI prey barcodes. Figure 1. Neighbor-joining tree of COI barcodes of Neotropical ants. The NJ tree was created in BOLD with the tool 'Taxon ID tree'. We included prey barcodes of army ants and reference barcodes of the projects ASLAM, BCIFO, and ARCAE in the analysis. The tree was based on MUSCLE alignment and Kimura’s two parameter model (K2P). We excluded all sequences with lengths of <400bp, sequences that contained stop codons in the reading frame, and records that were labeled as misidentifications or errors. Distinct BINs are highlighted in color and COI barcodes of army ant prey are underlined in black. Records in black letters were not given a BIN due to sequence lengths of <500bp. Scale bar represents p-distances. Tips of the tree include the following information: Species name, process ID, sample ID, country of collection, and BIN. Besides the 682 prey barcodes used in the main part of the article, we included in the tree 89 additional ant prey barcodes from a preliminary study about army ant diets (sample ID starts with “cvb…”). BOLD IDs and GenBank accession numbers for these records are given in Supplemental File 1. BOLD TaxonID Tree Title : Tree Result - Array Date : 26-Nov-2018 Data Type : Nucleotide Distance Model : Kimura 2 Parameter Marker : COI-5P Colourization : Barcode Cluster (BIN) Label : Sample ID Label : Process ID Label : Taxon Label : Country Label : Barcode Cluster (BIN) Sequence Count : 5688 Species count : 1181 Genus count : 98 Family count : 1 Unidentified : 79 BIN Count : 1779 Cover Page 1/1 [uASLAM, Mon Nov 26 21:16:46 2018 Page 1 of 57 5 % Neivamyrmex JTL014|ASLAM1594-11|CASENT0618821|Honduras|BOLD:ABA1296 Neivamyrmex JTL011|ASLAM1596-11|CASENT0618824|Honduras|BOLD:ABA1297 Neivamyrmex ADE0434|BCIFO1460-16|YB-BCI126211|Panama|BOLD:ADE0434 Neivamyrmex JTL015|ASLAM2377-13|CASENT0628944|Nicaragua Neivamyrmex JTL015|ASLAM2387-13|CASENT0628954|Nicaragua Neivamyrmex JTL015|ASLAM2389-13|CASENT0628956|Nicaragua|BOLD:ACI5983 Neivamyrmex ADE0944|BCIFO1462-16|YB-BCI137522|Panama|BOLD:ADE0944 Neivamyrmex moseri|ASLAM713-11|CASENT0614114|Honduras|BOLD:AAV4668 Neivamyrmex moseri|ASLAM714-11|CASENT0614116|Honduras|BOLD:AAV4668 Neivamyrmex spoliator|ASLAM1592-11|CASENT0618820|Honduras|BOLD:ABA0576 Neivamyrmex spoliator|BCIFO315-10|YB-BCI27578|Panama|BOLD:AAP3218 Neivamyrmex spoliator|BCIFO348-10|YB-BCI27554|Panama|BOLD:AAP3218 Neivamyrmex spoliator|BCIFO357-10|YB-BCI27524|Panama|BOLD:AAP3218 Neivamyrmex spoliator|BCIFO338-10|YB-BCI27530|Panama|BOLD:AAP3218 Neivamyrmex spoliator|BCIFO296-10|YB-BCI27511|Panama|BOLD:AAP3218 Neivamyrmex spoliator|BCIFO1458-16|YB-BCI129216|Panama|BOLD:AAP3218 Neivamyrmex spoliator|ASPAN160-10|MEKOU064582|Panama|BOLD:AAP3218 Neivamyrmex puerulus|BCIFO1100-13|JS130521_34|Panama|BOLD:ACJ4093 Neivamyrmex puerulus|BCIFO355-10|YB-BCI27427|Panama Neivamyrmex puerulus|BCIFO1093-13|JS130521_24|Panama|BOLD:ACJ4093 Neivamyrmex puerulus|FROGB050-16|RDT0131_244|Colombia|BOLD:ACJ4093 Neivamyrmex humilis|ASLAM455-11|CASENT0612220|Honduras|BOLD:ABV9692 Neivamyrmex humilis|ASLAM1938-12|CASENT0624966|Nicaragua|BOLD:ABV9692 Neivamyrmex humilis|ASPAN694-11|MEKOU_064006|Panama Neivamyrmex humilis|ASPAN695-11|MEKOU_160595|Panama Neivamyrmex adnepos|ASPAN696-11|MEKOU_160646|Panama|BOLD:AAZ8107 Neivamyrmex adnepos|ASPAN697-11|MEKOU_160644|Panama|BOLD:AAZ8107 Neivamyrmex adnepos|BCIFO1619-17|YB-BCI144362|Panama|BOLD:AAZ8107 Neivamyrmex adnepos|BCIFO1764-17|YB-BCI153948|Panama|BOLD:AAZ8107 Neivamyrmex adnepos|BCIFO1765-17|YB-BCI153924|Panama|BOLD:AAZ8107 Neivamyrmex adnepos|ASPAN693-11|MEKOU_064108|Panama|BOLD:AAZ8107 Neivamyrmex swainsonii|ASLAM1588-11|CASENT0619822|Nicaragua|BOLD:AAL7664 Neivamyrmex sp. 5YB|BCIFO319-10|YB-BCI27602|Panama Neivamyrmex sp. 5YB|BCIFO317-10|YB-BCI27587|Panama Neivamyrmex sp. 5YB|BCIFO316-10|YB-BCI27502|Panama Neivamyrmex impudens|ASLAM1587-11|CASENT0619821|Nicaragua|BOLD:AAF1400 Neivamyrmex gibbatus|BCIFO345-10|YB-BCI27454|Panama|BOLD:ACF0340 Neivamyrmex gibbatus|BCIFO342-10|YB-BCI27505|Panama|BOLD:ACF0340 Neivamyrmex gibbatus|BCIFO321-10|YB-BCI27549|Panama|BOLD:ACF0340 Neivamyrmex gibbatus|BCIFO288-10|YB-BCI24941|Panama|BOLD:ACF0340 Neivamyrmex gibbatus|BCIFO376-10|YB-BCI26775|Panama|BOLD:ACF0340 Neivamyrmex gibbatus|BCIFO341-10|YB-BCI27542|Panama|BOLD:ACF0340 Neivamyrmex pilosus|ASLAM449-11|CASENT0612209|Honduras|BOLD:AAM6940 Neivamyrmex pilosus|ASLAM1305-11|CASENT0618532|Nicaragua|BOLD:AAM6940 Neivamyrmex pilosus|BCIFO796-13|YB-BCI41032|Panama|BOLD:AAP9761 Neivamyrmex pilosus|BCIFO412-13|YB-BCI21789|Panama|BOLD:AAP9761 Neivamyrmex pilosus|BCIFO794-13|YB-BCI40434|Panama|BOLD:AAP9761 Neivamyrmex pilosus|BCIFO374-10|YB-BCI24760|Panama|BOLD:AAP9761 Neivamyrmex pilosus|BCIFO314-10|YB-BCI24578|Panama|BOLD:AAU3443 Neivamyrmex ADK8622|BCIFO1616-17|YB-BCI144166|Panama|BOLD:ADK8622 Neivamyrmex ADK8622|BCIFO1687-17|YB-BCI146817|Panama|BOLD:ADK8622 Neivamyrmex iridescens|DRYLO034-15|BCCISEC0010128|Ecuador|BOLD:ACY2241 Neivamyrmex iridescens|DRYLO113-15|BCPBA0110|Ecuador|BOLD:ACY2240 Neivamyrmex iridescens|DRYLO112-15|BCPBA0373|Ecuador|BOLD:ACY2240 Neivamyrmex iridescens|DRYLO111-15|BCPBA0356|Ecuador|BOLD:ACY2240 Neivamyrmex JTL013|ASLAM2060-12|CASENT0625244|Honduras|BOLD:ABV5356 Neivamyrmex pullus|BCIFO297-10|YB-BCI14750|Panama|BOLD:AAP9569 Neivamyrmex pullus|BCIFO349-10|YB-BCI27519|Panama|BOLD:AAP9569 Neivamyrmex pullus|BCIFO299-10|YB-BCI15319|Panama|BOLD:AAP9569 Neivamyrmex pullus|BCIFO300-10|YB-BCI11682|Panama|BOLD:AAP9569 Neivamyrmex pullus|BCIFO1103-13|JS130521_1|Panama|BOLD:ACJ4777 Neivamyrmex ADK8380|BCIFO1618-17|YB-BCI144324|Panama|BOLD:ADK8380 Neivamyrmex ADK8623|BCIFO1751-17|YB-BCI152674|Panama|BOLD:ADK8623 Neivamyrmex asper|ASLAM1526-11|CASENT0619760|Nicaragua|BOLD:ABA0577 Neivamyrmex texanus|ASLAM307-10|CASENT0614967|Honduras|BOLD:AAP3219 Neivamyrmex sumichrasti|ASLAM972-11|CASENT0617297|Honduras|BOLD:ADJ7352 Neivamyrmex sumichrasti|ASLAM295-10|CASENT0614946|Honduras|BOLD:ADJ7352 Neivamyrmex sumichrasti|ASLAM321-10|CASENT0615485|Honduras|BOLD:ADJ7352 Neivamyrmex sumichrasti|ASLAM393-11|CASENT0615107|Honduras|BOLD:ADJ7352 Neivamyrmex sumichrasti|ASLAM1476-11|CASENT0619710|Nicaragua|BOLD:ADJ7352 Neivamyrmex sumichrasti|ASLAM1695-11|CASENT0619644|Nicaragua|BOLD:ADJ7352 Neivamyrmex sumichrasti|ASLAM2403-13|CASENT0628970|Nicaragua|BOLD:ADJ7352 Neivamyrmex halidaii|BCIFO331-10|YB-BCI13041|Panama|BOLD:AAQ1273 Neivamyrmex halidaii|BCIFO1456-16|YB-BCI123988|Panama|BOLD:AAQ1273 Neivamyrmex halidaii|ASLAM1660-11|CASENT0619609|Nicaragua|BOLD:AAK9050 Neivamyrmex halidaii|ASLAM394-11|CASENT0615108|Honduras|BOLD:AAK9050 Neivamyrmex halidaii|ASLAM1103-11|CASENT0617641|Honduras|BOLD:AAK9050 Neivamyrmex halidaii|ASLAM1118-11|CASENT0617656|Honduras|BOLD:AAK9050 Neivamyrmex halidaii|ASLAM1210-11|CASENT0617998|Honduras|BOLD:AAK9050 Neivamyrmex halidaii|ARCAE121-17|T093-1|Ecuador|BOLD:ADH2761 Neivamyrmex halidaii|ARCAE122-17|T094_46575|Ecuador Cheliomyrmex morosus|ASLAM1855-12|CASENT0624883|Honduras|BOLD:ABV5027 Nomamyrmex hartigii|BCIFO310-10|YB-BCI27555|Panama|BOLD:AAP9522 Nomamyrmex hartigii|BCIFO373-10|YB-BCI26825|Panama|BOLD:AAP9522 Nomamyrmex hartigii|BCIFO347-10|YB-BCI27465|Panama|BOLD:AAP9522 Nomamyrmex hartigii|BCIFO311-10|YB-BCI24938|Panama|BOLD:AAP9522 Nomamyrmex hartigii|BCIFO312-10|YB-BCI28101|Panama|BOLD:AAP9522 Nomamyrmex hartigii|BCIFO1788-17|YB-BCI156484|Panama|BOLD:AAP9522 Nomamyrmex ACJ5993 esenbeckii wilsoni|BCIFO976-13|MEKOU012444|Panama|BOLD:ACJ5993 Nomamyrmex AAP9660 esenbeckii wilsoni|BCIFO344-10|YB-BCI27441|Panama|BOLD:AAP9660 Nomamyrmex AAP9660 esenbeckii wilsoni|BCIFO339-10|YB-BCI27544|Panama|BOLD:AAP9660 Nomamyrmex AAP9660 esenbeckii wilsoni|BCIFO330-10|YB-BCI26102|Panama|BOLD:AAP9660 Nomamyrmex AAP9660 esenbeckii wilsoni|BCIFO1329-13|MEKOU024258|Panama|BOLD:AAP9660 Nomamyrmex AAP9660 esenbeckii wilsoni|BCIFO329-10|YB-BCI26915|Panama|BOLD:AAP9660 Nomamyrmex AAP9660 esenbeckii wilsoni|ASPAN664-11|MEKOU_024259|Panama|BOLD:AAP9660 Eciton mexicanum|ASLAM1527-11|CASENT0619761|Nicaragua|BOLD:ABA0533 Eciton mexicanum|ASPAN651-11|MEKOU_024283|Panama|BOLD:AAZ8201 Eciton mexicanum|BCIFO1325-13|MEKOU024290|Panama|BOLD:AAZ8201 Eciton mexicanum|BCIFO1759-17|YB-BCI153950|Panama|BOLD:AAZ8201 Eciton mexicanum|ASPAN647-11|MEKOU_024291|Panama|BOLD:AAZ8201 [uASLAM, Mon Nov 26 21:16:46 2018 Page 2 of 57 Eciton mexicanum|BCIFO1325-13|MEKOU024290|Panama|BOLD:AAZ8201 Eciton mexicanum|BCIFO1759-17|YB-BCI153950|Panama|BOLD:AAZ8201 Eciton mexicanum|ASPAN647-11|MEKOU_024291|Panama|BOLD:AAZ8201 Eciton mexicanum|ASPAN662-11|MEKOU_024261|Panama|BOLD:AAZ8201 Eciton mexicanum|ASPAN663-11|MEKOU_024262|Panama|BOLD:AAZ8201 Eciton mexicanum|ASPAN665-11|MEKOU_024257|Panama|BOLD:AAZ8201 Eciton dulcium|BCIFO944-13|MEKOU012845|Panama|BOLD:ADN2801 Eciton dulcium|BCIFO1326-13|MEKOU024271|Panama|BOLD:AAZ9154 Eciton dulcium|ASPAN657-11|MEKOU_024272|Panama|BOLD:AAZ9154 Eciton vagans angustatum|ASLAM1572-11|CASENT0619806|Nicaragua|BOLD:AAI6193 Eciton vagans angustatum|ASLAM1659-11|CASENT0619608|Nicaragua|BOLD:AAI6191