Leader in Biomolecular Solutions for Life Science PRKAB2 Rabbit pAb Catalog No.: A13341 Basic Information Background Catalog No. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein A13341 kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non- catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that Observed MW monitors cellular energy status. In response to cellular metabolic stresses, AMPK is 35kDa activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in Calculated MW regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a 21kDa/30kDa positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been Category found for this gene. Primary antibody Applications WB,IHC,IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 5565 O43741 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human PRKAB2 (NP_005390.1). Synonyms PRKAB2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using PRKAB2 antibody (A13341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunofluorescence analysis of U2OS embedded rat brain using PRKAB2 embedded mouse brain using PRKAB2 cells using PRKAB2 antibody (A13341). Antibody (A13341) at dilution of 1:100 (40x Antibody (A13341) at dilution of 1:100 (40x Blue: DAPI for nuclear staining. lens). lens). Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..