A synthesis of existing data on larval rock lobster distribution in southern Australia Project 96/107 DEVELOPMENT CORPORATION Final Report to FRDC CS I RO Final Report the Project 96/107 B. Bruce, R. Bradford, 0. Griffin, C. Gardner and J. Young CSIRO Marine Laboratories Hobart ISBN: 0 643 062270 FRDC FIN.AL REPORT: PROJECT NO. 96/107 3, 5. 5.1 Scope and Rationale of 5.2 Sources of data {collaborating institutions) ......................................................................... 10 5.3 Sample Coverage ............................................................................................................... i2 5.3.1 Geographic ................................................................................................................................................. 12 5.3.2 Annual ........................................................................................................................................................ 13 5.3.3 Seasonal .................................................................................................................................................... 13 5.4 Net Systems: ...................................................................................................................... 14 5.4.1 Surface sampling ........................................................................................................................................ 14 Surface net ...................................................................................................................................... 14 Ring net. .......................................................................................................................................... 14 Bongo net ........................................................................................................................................ 14 5.4.2 Depth stratified sampling ............................................................................................................................ 14 EZ (BIONESS) net ......................................................................................................................... 14 MIDOC net ..................................................................................................................................... 15 5.4.3 Vertically integrated sampling .................................................................................................................... i5 Bongo net. ....................................................................................................................................... 15 Vertical haul net. ............................................................................................................................. 16 Ring net. .......................................................................................................................................... 16 5.5 Sample Processing ............................................................................................................. 16 5.5. 1 At sea ......................................................................................................................................................... 16 5.5.2 Laboratory .................................................................................................................................................. 16 FRDC FINAL REPORT: PROJECT NO. 96/107 5.6 Physical oceanographic data sets ....................................................................................... 17 5.7 Identification of phyllosoma ................................................................................................. 17 5.7.1 Morphological ...................................................................... " ..................................................................... 18 5.7.2 Genetic Analysis ......................................................................................................................................... 19 DNA Extraction .............................................................................................................................. 19 Polymerase Chain Reaction (PCR) Amplification .......................................................................... 20 \ 5.7.3 Results of genetic analyses ........................................................................................................................ 20 DNA Isolation ................................................................................................................................. 20 Polymerase Chain Reaction (PCR) Amplification .......................................................................... 20 DNA Sequencing ............................................................................................................................ 20 5.7.4 Discussion of genetic analyses .................................................................................................................. 21 DNA Isolation ................................................................................................................................. 21 6. PROJECT RESULTS ........................................................................................................... 21 6.1 Geographic distribution of phyllosoma ................................................................................ 21 6.1.1 Introduction ................................................................................................................................................. 21 6.1.2 Results ....................................................................... .'............................................................................... 22 Stage distribution Jasus edwardsii ............................................................................................... 22 Jasus verreauxi phyllosoma ............................................................................................................ 22 Seasonal distribution ....................................................................................................................... 22 Tasmanian waters ........................................................................................................................... 23 Distribution of pueruli .................................................................................................................... 23 6.1.4 Discussion .................................................................................................................................................. 24 Puerulus .......................................................................................................................................... 25 Jasus edwardsii ............................................................................................................................... 25 Jasus verreauxi ................................................................................................................................ 25 6.2 Vertical Distribution ............................................................................................................. 26 6.2.1 Introduction ................................................................................................................................................. 26 6.2.2 Methods ...................................................................................................................................................... 26 6.3.3 Results ....................................................................................................................................................... 27 MIDOC Net: ................................................................................................................................... 27 J asus edwardsii ............................................................................................................................... 28 J. edwardsii ..................................................................................................................................... 28 EZ Net: ........................................................................................................................................... 28 Surface net ...................................................................................................................................... 29 Combined Depth Distribution ......................................................................................................... 29 2 FRDC FINAL REPORT: PROJECT NO. 96/107 6.3A Discussion .................................................................................................................................................. 30 6.4.1 32 6.4.2 Metnoa:s .. Larval l . Occurrence of 2. Occurrence of sett!ernent. .............................................................. 33 3. Growth rate data from u...,~U¼~" ............................................................................................. 33 6.4.3 Results ....................................................................................................................................................... 33 Larval Duration ............................................................................................................................... 33 l. Occurrence of stages ................................................................................................................... 33 2. Appearance of stage 1