ISSN 2336-3193 Acta Mus. Siles. Sci. Natur., 65: 1-13, 2016 DOI: 10.1515/cszma-2016-0001 Strongylophthalmyiidae, Tanypezidae and Megamerinidae (Diptera) in the Czech Republic and Slovakia: current state of knowledge Jindřich Roháček Strongylophthalmyiidae, Tanypezidae and Megamerinidae (Diptera) in the Czech Republic and Slovakia: current state of knowledge. – Acta Mus. Siles. Sci. Natur. 65: 1-13, 2016. Abstract: A review of the occurrence of representatives of the families Strongylophthalmyiidae, Tanypezidae and Megamerinidae (Diptera) in the Czech Republic and Slovakia is presented based on literary data and recently obtained records. Strongylophthalmyia pictipes Frey, 1935 (Strongyl- ophthalmyiidae) is a new addition to the fauna of the Czech Republic. Its record from the Jizerské hory Mts is the second from Central Europe and represents a new southwesternmost distribution limit of the species. Biology, distribution, faunistics in the Czech Republic and Slovakia and importance for nature conservation (with evaluation of degree of jeopardy) are discussed for all species, including also Strongylophthalmyia ustulata (Zetterstedt, 1847), Tanypeza longimana Fallén, 1820 (Tany- pezidae) and Megamerina dolium (Fabricius, 1805 (Megamerinidae). Key words: Diptera, Strongylophthalmyiidae, Tanypezidae, Megamerinidae, Strongylophthalmyia pictipes, biology, distribution, Czech Republic, Slovakia Introduction The recent discovery of a second species of the genus Strongylophthalmyia (Strongyl- ophthalmyiidae) in the Czech Republic (see below) stimulated a revision of all available material of the genus from this country as well as from Slovakia. On this occasion also the occurrence of two other acalyptrate families, Tanypezidae and Megamerinidae, represented by single species in Central Europe, has been studied because they both, like Strongylophthalmyiidae, have hitherto been scarcely recorded from the Czech Republic and, particularly, from Slovakia. Therefore, this study is aimed at a summary of all available information (both published and based on newly obtained records) about the occurrence of all species of the three families in these countries. Formerly, only one species of each of them were known from the Czech Republic and Slovakia, viz. Strongylophthalmyia ustulata (Zetterstedt, 1847), Tanypeza longimana Fallén, 1820 and Megamerina dolium (Fabricius, 1805), as presented in the electronic checklist version 2 (Roháček (2009c,d,e). All published (relatively scarce) records from the above countries are listed below under each of these species. Most of them have been revised during the course of this study but much more additional material has been newly studied. The data obtained from the material examined are discussed for each species as regards the information on the biology, distribution, occurrence within the Czech Republic and Slovakia and degree of jeopardy. Although only a single species, Strongylophthalmyia pictipes Frey, 1935, is added to the fauna of the Czech Republic, this record is very important, confirming that two species of Strongylophthalmyiidae in fact occur in Central Europe. S. pictipes can be easily recognized from its more common congener, S. ustulata, in having wing with more distinct dark apical spot, all femora (fore femora only dorsally) dark in apical third to half, also tibiae largely darkened (Figs 1, 3, compare with Fig. 4) and broad, short and blackish palpus. For other differences see Palazcyk et al. (2013). 1 Material and methods All available material of flies of the families Strongylophthalmyiidae, Tanypezidae and Megamerinidae from the Czech Republic and Slovakia has been examined. It is deposited in various collections as follows: BMSC – Blatské muzeum v Soběslavi, Czech Republic, JMB – collection of Dr. Jan Máca, Ph.D., České Budějovice; KMVC – Museum of East Bohemia, Hradec Králové, Czech Republic; MBP – collection of Prof. Dr. Miroslav Barták, CSc., Praha; NMPC – National Museum, Praha; SMLC – Severočeské muzeum, Liberec; SMOC - Silesian Museum, Opava, Czech Republic. Revised literary records are indicated by „!“ in parantheses behind the date of the respective reference. Specimens have been examined using two types of binocular stereoscopic microscopes (Reichert, Olympus). When necessary male genitalia and female terminalia were examined after detachment, treating in hot 10% KOH, washing in water and dissection of the whole abdomen in a drop of glycerine under a binocular microscope. After examination, all parts were transferred to a small plastic tube in glycerine and pinned below the respective specimens. Some of the collected adults were retained alive in plastic tubes to be photographed shortly after being captured in special boxes by means of a digital camera Canon EOS 60D with a macro lens (Canon MP-E 65 mm 1–5×) and ring macro flash (Canon MR-14EX). Results STRONGYLOPHTHALMYIIDAE Strongylophthalmyia pictipes Frey, 1935 (Figs 1, 3) Material examined: CZECH REPUBLIC: N Bohemia: Jizerské hory Mts, Horní Maxov-Malá Strana res., 50°45'58''N, 15°12'04''E, 715 m, netted from fallen birch trunks, 15.vii.2015, 1♂, J. Roháček leg. (SMOC, photographed). Biology: Krivosheina (1981) reported on specimens of S. pictipes reared by Babushkin from larvae found under the bark of rotting aspen in Russia (Buryatia) but the immatures remain undescribed. In SE Poland (Bieszczady Mts: Terebowiec valley) a total of 12 adults (all males) of this species were collected in wet deciduous forest (Alnetum incanae) on a dead trunk of aspen (Populus tremula) lying on the ground near a stream from 29 June to 3 August (Palazcyk et al. 2013). However, the only male found in the Jizerské hory Mts (N Bohemia) was swept from fallen dead trunks of a birch (Betula sp.) on the ground in a humid mixed forest (Fig. 2). Distribution: This little known species seems to be widespread largely in the northern belt of the Palaearctic Region, see Palazcyk et al. (2013). According to the catalogue presented by the latter authors, S. pictipes is known from the following countries: Norway, Sweden, Finland, Poland, Russia: Moscow region, Buryatia, Amur district. The only previous record from Central Europe originates from Poland (Bieszczady Mts), and, consequently, the first finding from the Czech Republic (see above) is not only the second from Central Europe but it also represents a new southwestern distribution limit of this species. Discussion: Based on Polish record from the Bieszczady Mts, Palazcyk et al. (2013) discussed S. pictipes as a Boreomontane species having in this Carpathian locality a relict population possibly of the Late-Pleistocene (Alleröd) or Early-Holocene (Preboreal) origin. The single record from the Jizerské hory Mts also indicates that S. pictipes occurs very locally in montane forested habitats of Central Europe and, hence, corroborates its Boreomontane distribution in Europe. Judging from its relict distribution, S. pictipes is surely the most endangered species (of all here discussed taxa) not only in the Czech Republic but in the whole of Central Europe. Strongylophthalmyia ustulata (Zetterstedt, 1847) (Fig. 4) Material examined: CZECH REPUBLIC: S Bohemia: Kašperské Hory 2 km SW, nr. Losenice, 49.08 N / 13.33 E, 600 m, 9.vii.1994, 1♀, M. Barták leg. (V. Martinek det. 1994, KMVC); Šumava Mts, Malá niva, 49.08' N 13.33' E, peat-bog, Malaise trap, 1♀, M. Barták & J. Roháček leg. (SMOC). N Bohemia: Josefův Důl, Jedlový důl res., sweeping, 12.vii.2005, 1♀, J. Preisler leg. (JMB); Jizerské hory Mts, Josefův Důl, Jedlový důl res., 2 Figs 1–2: Strongylophthalmyia pictipes Frey and its habitat in the Czech Republic. 1 – living male (in sublateral view) from Horní Maxov-Malá Strana res. (Czech Republic: Jizerské hory Mts); 2 – habitat with rotting birch trunks where this specimen was collected. Photo by J. Roháček. 3 50°47'24''N, 15°14'29''E, 700 m, sweeping vegetation along brook, 21.vii.2013, 1♀, 15.vii.2015, 2♀, J. Roháček leg. (SMOC, 1♀ photographed); Jizerské hory Mts, Kořenov, Jizera river, 50°46'18.3''N, 15°22'56.2''E, 640 m, sweeping and individual coll. on river banks, 24.vii.2013, 3♀; Jizerské hory Mts, Kořenov, Jizerka, Bukovec NR, Starý lom, 50°48'44''N, 15°21'39.9''E, 925 m, sweeping undergrowth, 20.vii.2015, 1♀, all M. Tkoč leg. (NMPC); Jizerské hory Mts, Rejdice, potok Ješkrabec (5258), 650 m, emergent trap, 16.viii.-1.9.2015, 1♀, J. Preisler & P. Vonička leg. (SMLC); Holany env. (5352), distr. Česká Lípa, sweeping, 19.vii.2006, 1♂, J. Preisler leg. (coll. MBP). E Bohemia: Orlické hory Mts, Popelov, L břeh Zdobnice, 1.vii.1982, 1♀ (J. Roháček det. 1984); Orlické hory Mts, Pěčín, valley of Zdobnice (5864), 440 m, 13.vii.1995, 1♀, B. Mocek leg.; Sněžné v Orlických horách, 21.viii.1965, 1♀, V. Martinek leg. (and det. 1991); Železné hory Mts, Třemošnice- Hedvikovské údolí (6159), 29.vii.1994, 1♀, B. Mocek leg. (V. Martinek det. 1997) (all in KMVC). S Moravia: Soutok, Ranšpurk res. (distr. Břeclav), sweeping undergrowth of deciduous forest, 14.vi.2000, 1♀, J. Roháček leg. (SMOC); Bílé Karpaty Mts, Štítná-Žírce, 49°2'46-56"N, 17°59'6-20"E, 400-450 m, mass sweeping, 4.viii.2013, 1♂; S Moravia: Bílé Karpaty Mts, Štítná, „U brodu“, 19.vii.2015, 1♀, both M. Tkoč leg. (NMPC). N Moravia: Karlova Pláň-Karlovec (distr. Bruntál), sweeping undergrowth of alder forest, 30.vi.1987, 1♀, 5.viii.1987, 1♀; Deštné env., Kamenná hora Mt. env. (distr. Opava), sweeping undergrowth of deciduous forest, 27.vi.1996, 1♀; Hněvošice, Hněvošický háj res. (distr. Opava), sweeping undergrowth of deciduous forest, 29.vi.1995, 1♀; Hradec nad Moravicí-Záviliší, sweeping undergrowth of mixed forest, 26.vi.1994, 2♂; N Moravia: Moravskoslezské Beskydy Mts, Dolní Bečva-Horní Rozpité, sweeping undergrowth of mixed forest, 30.vi.1994, 1♀, all J. Roháček leg. (SMOC). SLOVAKIA: C Slovakia: Poľana Mts, Čierny Potok valley, sweeping undergrowth of alder forest, 8.vii.2000, 1♂; Poľana Mts, Hrončecký grúň, sweeping undergrowth of mixed forest, 12.vi.1999, 1♀, on rotting tree trunks, 4.vii.2000, 1♀; Poľana Mts, Spády waterfall, sweeping vegetation along brook, 4.vii.2000, 1♀, all J. Roháček leg (SMOC).