Product Information Sheet for NR-19556 Salmonella enterica subsp. enterica, Strain supplemented with 15% glycerol. ® Ty2 (Serovar Typhi), Gateway Clone Set, Packaging/Storage: Recombinant in Escherichia coli, Plate 35 NR-19556 was packaged aseptically in a 96-well plate. The product is provided frozen and should be stored at -80°C or Catalog No. NR-19556 colder immediately upon arrival. For long-term storage, the This reagent is the tangible property of the U.S. Government. vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided. For research use only. Not for human use. Growth Conditions: Media: Contributor: LB broth or agar containing 50 µg/mL kanamycin. Pathogen Functional Genomics Resource Center at the J. Incubation: Craig Venter Institute Temperature: 37°C Atmosphere: Aerobic Manufacturer: Propagation: BEI Resources 1. Scrape top of frozen well with a pipette tip and streak onto agar plate. Product Description: 2. Incubate the plates at 37°C for 24 hours. Clone plates are replicated using a BioMek® FX robot. Production in the 96-well format has increased risk of cross- Citation: contamination between adjacent wells. Individual clones Acknowledgment for publications should read “The following should be purified (e.g. single colony isolation and reagent was obtained through BEI Resources, NIAID, purification using good microbiological practices) and NIH: Salmonella enterica subsp. enterica, Strain Ty2 sequence-verified prior to use. BEI Resources only confirms (Serovar Typhi), Gateway® Clone Set, Recombinant in the clone plate orientation and viability of randomly picked Escherichia coli, Plate 35, NR-19556.” clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor. Biosafety Level: 1 Appropriate safety procedures should always be used with The Salmonella enterica subsp. enterica (S. enterica subsp. this material. Laboratory safety is discussed in the following ® enterica), strain Ty2 (serovar Typhi), Gateway clone set publication: U.S. Department of Health and Human Services, consists of approximately 3380 sequence validated clones Public Health Service, Centers for Disease Control and from S. enterica subsp. enterica, strain Ty2, cloned in Prevention, and National Institutes of Health. Biosafety in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading Microbiological and Biomedical Laboratories. 5th ed. frame was constructed in vector pDONR221 (Invitrogen) Washington, DC: U.S. Government Printing Office, 2009; see with an ATG start codon and no stop codon. The sequence www.cdc.gov/biosafety/publications/bmbl5/index.htm. was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than Disclaimers: 0.2%. Detailed information about each clone is shown in You are authorized to use this product for research use only. Table 1. It is not intended for human use. ® Information related to the use of Gateway Clones can be Use of this product is subject to the terms and conditions of obtained from Invitrogen. Recombination was facilitated the BEI Resources Material Transfer Agreement (MTA). The through an attB substrate (attB-PCR product or a linearized MTA is available on our Web site at www.beiresources.org. attB expression clone) with an attP substrate (pDONR221) to create an attL-containing entry clone. The entry clone While BEI Resources uses reasonable efforts to include contains recombinational cloning sites, attL1 and attL2 to accurate and up-to-date information on this product sheet, facilitate gene transfer into a destination vector, M13 forward neither ATCC® nor the U.S. Government makes any and reverse priming sites for sequencing and a kanamycin warranties or representations as to its accuracy. Citations resistance gene for selection. Please refer to the Invitrogen from scientific literature and patents are provided for Gateway® Technology Manual for additional details. informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been Plate orientation and viability were confirmed for NR-19556. confirmed to be accurate. Material Provided: This product is sent with the condition that you are Each inoculated well of the 96-well plate contains responsible for its safe storage, handling, use and disposal. approximately 60 µL of E. coli culture (strain DH10B-T1) in ATCC® and the U.S. Government are not liable for any Luria Bertani (LB) broth containing 50 µg/mL kanamycin damages or injuries arising from receipt and/or use of this BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2013 American Type Culture Collection (ATCC). All rights reserved. NR-19556_20NOV2013 Page 1 of 4 Product Information Sheet for NR-19556 product. While reasonable effort is made to ensure its derivatives must contact the contributor to determine if a authenticity and reliability of materials on deposit, the U.S. license is required. U.S. Government contractors may need Government, ATCC®, their suppliers and contributors to BEI a license before first commercial sale. Resources are not liable for damages arising from the misidentification or misrepresentation of products. References: 1. Deng, W., et al. “Comparative Genomics of Salmonella Use Restrictions: enterica serovar Typhi strains Ty2 and CT18.” J. This material is distributed for internal research, non- Bacteriol. 185 (2003): 2330-2337. PubMed: 12644504. commercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as ATCC® is a trademark of the American Type Culture performed under a U.S. Government contract, individuals Collection. contemplating commercial use of the material, its products or Table 1: Salmonella enterica subsp. enterica, Strain Ty2 (Serovar Typhi), Gateway® Clone Set, Recombinant in Escherichia coli, Plate 35 (ZSTEI)1 Well ORF Accession Average Depth of Clone Locus ID Description Position Length Number Coverage 98935 A01 t3922 oligopeptidase A 2077 NP_807535.1 4.668272 98796 A02 t1282 secretion system apparatus protein SsaV 2080 NP_805085.1 3.225481 98823 A03 t1033 carboxy-terminal peptidase 2083 NP_804851.1 3.983677 98828 A04 t0998 protease 2 2086 NP_804821.1 4.786194 98907 A05 t3184 hypothetical protein t3184 2086 NP_806862.1 4.412752 99021 A06 t1845 DNA helicase IV 2089 NP_805614.1 3.235998 98729 A07 t0202 iron-hydroxamate transporter permease 2092 NP_804084.1 4.218451 98915 A08 t2784 pathogenicity island 1 effector protein 2092 NP_806485.1 4.027247 98843 A09 t2798 virulence-associated secretory protein 2092 NP_806499.1 3.723709 98927 A10 t3907 hypothetical protein t3907 2095 NP_807521.1 4.871122 98739 A11 t0356 polyphosphate kinase 2101 NP_804226.1 3.34793 98719 A12 t4168 type I secretion protein 2101 NP_807772.1 4.814374 98815 B01 t3864 glucyl-tRNA synthetase subunit beta 2104 NP_807483.1 4.411597 formate hydrogenlyase system 98971 B02 t2761 2113 NP_806462.1 4.539991 transcriptional activator 99007 B03 t3774 ATP-dependent DNA helicase RecG 2116 NP_807397.1 3.724953 99061 B04 t3993 4-alpha-glucanotransferase 2116 NP_807603.1 3.836011 98951 B05 t4260 hypothetical protein t4260 2125 NP_807858.1 4.312941 98835 B06 t4131 lipoprotein 2131 NP_807735.1 4.217738 98699 B07 t0018 chitinase 2134 NP_803903.1 4.116682 98879 B08 t2268 carbon starvation protein A 2140 NP_806010.1 4.149533 99024 B09 t0737 hypothetical protein t0737 2146 NP_804581.1 3.997204 bifunctional (p)ppGpp synthetase II/ 98957 B10 t3776 guanosine 3’,5’-bis pyrophosphate 3’- 2146 NP_807399.1 3.264212 pyrophosphohydrolase 98933 B11 t4059 elongation factor G 2149 NP_807666.1 4.167985 98919 B12 t1497 TonB-dependent receptor 2155 NP_805283.1 4.115081 98867 C01 t4011 hypothetical protein t4011 2167 NP_807621.1 3.187356 98851 C02 t3200 polynucleotide phosphorylase 2170 NP_806878.1 4.430415 anaerobic ribonucleoside triphosphate 98875 C03 t4486 2173 NP_808073.1 3.489646 reductase 98944 C04 t0236 lysine decarboxylase 2176 NP_804116.1 3.444853 multifunctional fatty acid oxidation 98695 C05 t0476 2182 NP_804334.1 3.569203 complex subunit alpha 98831 C06 t1505 virulence effector protein 2182 NP_805291.1 4.648029 98707 C07 t4192 formate dehydrogenase H 2182 NP_807796.1 4.415215 BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2013 American Type Culture Collection (ATCC). All rights reserved. NR-19556_20NOV2013 Page 2 of 4 Product Information Sheet for NR-19556 Well Locus ORF Accession Average Depth Clone Description Position ID Length Number of Coverage 98787 C08 t1847 hypothetical protein t1847 2188 NP_805616.1 4.686929 98999 C09 t0756 tyrosine kinase 2194 NP_804600.1 3.790793 bifunctional acyl-[acyl carrier protein] synthetase/2- 98848 C10 t2919 2194 NP_806613.1 3.137192 acylglycerophosphoethanolamine acyltransferase 98751 C11 t3346 DNA-dependent helicase II 2197 NP_807014.1 4.282658 99055 C12 t3088 hypothetical protein t3088 2206 NP_806772.1 3.586582 98811 D01 t3982 glycogen branching protein 2221 NP_807592.1 4.298964 multifunctional fatty acid oxidation 98820 D02 t3315 2224 NP_806983.1 1.957734 complex subunit alpha zinc/cadmium/mercury/lead-transporting 98723 D03 t3946 2233 NP_807557.1 3.521272 ATPase 98735 D04 t2845 hypothetical protein t2845 2242 NP_806542.1 4.4157 98779 D05 t0354 hypothetical protein t0354 2248 NP_804224.1 4.516904 98979 D06 t2061 hypothetical protein t2061 2257 NP_805824.1 4.32521 98711 D07 t2744 hydrogenase maturation protein 2275 NP_806445.1