SAN TA C RUZ BI OTEC HNOL OG Y, INC . ZFYVE27 (S-15): sc-132148 BACKGROUND APPLICATIONS Zinc finger FYVE domain-containing protein 27 (ZFYVE27), also known as ZFYVE27 (S-15) is recommended for detection of ZFYVE27 isoforms 1-5 of SPG33, is a 411 amino acid member of the FYVE-finger family of proteins. mouse, rat and human origin by Western Blotting (starting dilution 1:200, The FYVE domain is a cysteine-rich domain of about 70 amino acids that dilution range 1:100-1:1000), immunofluorescence (starting dilution 1:50, plays a role in the endosomal localization of the FYVE-finger proteins, and dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, a majority of these proteins serve as regulators of endocytic membrane traf - dilution range 1:30-1:3000); non cross-reactive with other ZFYVE family ficking. ZFYVE27, a multi-pass membrane protein, is an endosomal protein members. that binds to Spastin, a protein that is primarily involved in microtubule dy- ZFYVE27 (S-15) is also recommended for detection of ZFYVE27 isoforms 1-5 namics and severing, vesicular trafficking and endosomal trafficking. Muta- in additional species, including bovine. tions in the gene encoding ZFTVE27 affect neuronal intracellular trafficking in the corticospinal tract and are thought to lead to hereditary spastic para - Suitable for use as control antibody for ZFYVE27 siRNA (h): sc-90825, plegia (HSP), a neurodegenerative disorder, characterized by progressive ZFYVE27 siRNA (m): sc-155606, ZFYVE27 shRNA Plasmid (h): sc-90825-SH, paralysis of the legs, which is caused by impaired axonal transport. Five ZFYVE27 shRNA Plasmid (m): sc-155606-SH, ZFYVE27 shRNA (h) Lentiviral isoforms of ZFYVE27 exist as a result of alternative splicing events. Particles: sc-90825-V and ZFYVE27 shRNA (m) Lentiviral Particles: sc-155606-V. REFERENCES Molecular Weight of ZFYVE27: 46 kDa. 1. Fink, J.K. 1997. Advances in hereditary spastic paraplegia. Curr. Opin. Positive Controls: Jurkat whole cell lysate: sc-2204. Neurol. 10: 313-318. 2. Mannan, A.U., et al. 2006. ZFYVE27 (SPG33), a novel spastin-binding RECOMMENDED SECONDARY REAGENTS pro tein, is mutated in hereditary spastic paraplegia. Am. J. Hum. Genet. To ensure optimal results, the following support (secondary) reagents are 79: 351-357. recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 3. Shirane, M., et al. 2006. Protrudin induces neurite formation by directional (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey membrane trafficking. Science 314: 818-821. anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 4. Online Mendelian Inheritance in Man, OMIM™. 2006. Johns Hopkins sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- University, Baltimore, MD. MIM Number: 610243. World Wide Web URL: rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- http://www.ncbi.nlm.nih.gov/omim/ 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) 5. Ivanova, N., et al. 2007. Hereditary spastic paraplegia 3A associated with with UltraCruz™ Mounting Medium: sc-24941. axonal neuropathy. Arch. Neurol. 64: 706-713. 6. Martignoni, M., et al. 2008. The role of ZFYVE27/protrudin in hereditary STORAGE spastic paraplegia. Am. J. Hum. Genet. 83: 127-128. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. 7. Zhao, G.H., et al. 2008. A novel candidate locus on chromosome 11p14.1-p11.2 for autosomal dominant hereditary spastic paraplegia. RESEARCH USE Chin. Med. J. 121: 430-434. For research use only, not for use in diagnostic procedures. CHROMOSOMAL LOCATION PROTOCOLS Genetic locus: ZFYVE27 (human) mapping to 10q24.2; Zfyve27 (mouse) mapping to 19 C3. See our web site at www.scbt.com or our catalog for detailed protocols and support products. SOURCE ZFYVE27 (S-15) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an extracellular domain of ZFYVE27 of human ori gin. PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-132148 P, (100 µg pep tide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.