Charles University in Prague, Faculty of Science __________________________________________________________________ 22nd HELMINTHOLOGICAL DAYS 2015 Programme & Abstracts Editors: Terezia Perháčová, Libuše Turjanicová & Vladimír Skála Prague 2015 Conference organizers: Laboratory of Helminthology, Department of Parasitology, Faculty of Science, Charles University in Prague Helminthological Section Czech Society for Parasitology Venue and date of the conference: 22nd Helminthological Days, Recreational facility FTVS UK, Dvorce near Stráž nad Nežárkou, Czech Republic, 4.-8. May 2015 © 2015 Univerzita Karlova v Praze a Česká parazitologická společnost ISBN 978-80-7444-032-8 Programme PROGRAMME OF 22nd HELMINTHOLOGICAL DAYS MONDAY, MAY 4 15.00 - 18.00 Arrival and registration of participants 18.00 - 18.30 Dinner 19.00 - 00.00 Get-together-evening TUESDAY, MAY 5 08.00 - 09.00 Breakfast 09.00 - 09.10 Opening ceremony: H. Dvořáková 09.10 - 09.40 Invited talk: W. Haas: Strategies of parasitic worms to find, recognize and invade their hosts (Chairman: P. Horák) Session I Interactions between Trichobilharzia spp. and their hosts (Chairman: P. Horák) 09.40 - 09.55 V. Skála, P. Horák: Haemocyte extracellular traps in the snail Lymnaea stagnalis (Lymnaeidae): do they occur and are they used as a defence response against trematodes? PhD 09.55 - 10.10 V. Krčmářová, J. Bulantová, P. Horák: Interactions of neurotropic larvae of bird schistosome Trichobilharzia regenti and immune cells of host central nervous system. BSc/MSc 10.10 - 10.25 T. Macháček, L. Panská, P. Horák: Antigens of the neuropathogenic fluke Trichobilharzia regenti elicit nitric oxide and proinflammatory cytokine secretion by glial cells in vitro. BSc/MSc 10.25 - 10.40 L. Vlčková, P. Horák: Interactions of the eggs and miracidia of Trichobilharzia regenti with the duck. BSc/MSc 10.40 - 11.00 Coffee break Session II Biodiversity and variability of helmints (Chairman: R.Kuchta) 11.00 - 11.15 N. Kmentová, M. Gelnar, M. Mendlová, M.Van Steenberge, S. Koblmüller, M. P. M Vanhove: Intraspecific variation of Cichlidogyrus (Monogenea, Dactylogyridae) in Lake Tanganyika. BSc/MSc 11.15 - 11.30 T. A. Kuzmina, T. R. Spraker, E. T. Lyons, R. Kuchta, O. Lisitsyna, O. Kudlai: Biodiversity of gastrointestinal parasite community in northern fur seals (Callorhinus ursinus) on St. Paul Island, Alaska. 11.30 - 11.45 V. Michálková, M. Reichard, M. Ondračková: Metazoan parasites of killifish from Mosambique. PhD 11.45 - 12.00 M. Pravdová, R. Blažek, M. Gelnar, E. Řehulková: Diversity of monogeneans parasitizing freshwater fishes of the Nile River in Sudan: current state and prospects. BSc/MSc 12.00 - 12.15 K. Leštinová, R. Kuchta: Morphological and morphometrical analyses of the eggs of human-infecting diphyllobothriid cestodes. PhD 12.15 - 12.30 P. Zahradníčková, M. Barson, W. J. Luus-Powell, I. Přikrylová: An evaluation of different discriminative approaches used for discrimination of cryptic species. PhD 12.30 - 13.00 Lunch Programme Session III Environmental parasitology, epizootology (Chairman: M. Gelnar) 13.30 - 13.45 J. Bulantová, L. Turjanicová, M. Vaščiková, R. Leontovyč, M. Chanová, P. Horák: Velký Bolevecký pond: story of an outdoor laboratory for avian schistosomes. 13.45 - 14.00 J. S. Hernandes Orts, T.Scholz, J. Brabec, T. Kuzmina, R. Kuchta: High morphological plasticity and global geographical distribution of the Pacific broad tapeworm Adenocephalus pacificus (syn. Diphyllobothrium pacificum): molecular and morphological survey. 14.00 - 14.15 O. Kudlai: Morphological and molecular approaches to the study of digenean larvae from the Curonian lagoon in Lithuania. PhD 14.15 - 14.30 L. Raisingerová, Š. Mašová, A. Vetešníková Šimková, R. Šanda, J. Vukić, M. Gelnar: Basic epidemiological data of anisakid nematode larvae of gobiid fishes (Gobiidae, Actinopterygii) from northern Adriatic Sea. BSc/MSc 14.30 - 14.45 T. Sosnová, M. Kašný: Fascioloid flukes of livestock and wild animals in the Czech Republic. BSc/MSc 14.45 - 15.15 Coffee break Session IV Host-parasite interactions and pathogenesis (Chairman: J. Brabec) 15.15 - 15.30 Z. Čadková, B. Horáková, V. Sloup, J. Száková, J. Vadlejch, I. Langrová: Are rodent tapeworms suitable accumulative indicators for their host´s risk element exposure? 15.30 - 15.45 V. Krasnovyd, A. Šimková: Influence of hybridization on the fish host conditions and parasite infection. 15.45 - 16.00 T. Pakosta, A. Vetešníková Šimková: Parasite load and immunity in diploid-polyploid complex of Carassius gibelio. PhD 16.00 - 16.15 T. R. Spraker, T. A. Kuzmina, E. T. Lyons, R. DeLong: Pathogenesis of hookworm/enteritis complex with bacteremia caused by Uncinaria spp. in California sea lions and Northern fur seals. Poster session (Chairman: R. Leontovyč) 16.30 - 17.30 2 min presentations and individual poster discussion N. Bernardová, M. Chanová: Long-term murine cerebral toxocariasis. BSc/MSc K. Civáňová, L. Gettová, A. Gilles, A. Šimková: Effect of a co-existence of invasive Barbus barbus and local Barbus meridionalis on the composition of metazoan parasite communities. T. A. Kuzmina, V. A. Kharchenko, N. S. Zvegintsova: Strongylides (Nematoda; Strongylidae) of wild and domestic equids in Ukraine: biodiversity and parasite community structure. V. V. Pavliková, M. Chanová: Helminths and immune-mediated disorders (hypothesis and plans). BSc/MSc I. Přikrylová, I. Walter, W. J. Luus-Powell, M. Barson: Monogenean parasites from Pseudocrenilabraus philander (Weber, 1897) in the middle Limpopo River Basin (South African region). I. Přikrylová, Š. Mašová, M. M. Matla, M. Gelnar, W. J. Luus-Powell: The revision of the genus Afrodiplozoon (Khotenovsky, 1981.) (Monogenea: Diploziodae ). J. Sedláková, M. Borkovcová: Endoparasites of wild animals of vineyard and agricultural landscape in the South Moravia. PhD Y. Yu. Syrota, V. A. Kharchenko, T. A. Kuzmina: Study of the nematode fauna in waterfowls on the Ukrainian Polesie. PhD 18.00 - 18.30 Dinner 20.00 - 00.00 Campfire night Programme WEDNESDAY, MAY 6 08.00 - 08.45 Breakfast 08.45 - 09.15 Invited talk: J. P. Dalton: Understanding Fasciola hepatica relationship with it´s host using genomics, transcriptomics and proteomics, with basic biology. (Chairman: M. Kašný) Session V Molecular biology (Chairman: M. Kašný) 09.15 - 09.30 H. Dvořáková, L. Jedličková, M. Kašný, P. Brož, J. Ilgová, M. Gelnar, L. Mikeš: Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists. PhD 09.30 - 09.45 D. Jirsová, M. Gelnar, M. Kašný: Monogenea … what else can be done? 09.45 - 10.00 R. Leontovyč, N. Young, P. Horák, G. Robin, M. Kašný: Comparative study of transcriptome profiles of cercariae/schistosomula of bird schistosome Trichobilharzia regenti. PhD 10.00 - 10.15 R. Šašková, R. Lenontovyč, L. Ulrychová, J. Dvořák, P. Horák, M. Kašný: The expression of cathepsin L in developmental stages of Fascioloides magna. BSc/MSc 10.15 - 10.30 L. Škorpíková, B. Koudela, J. Ilgová, M. Gelnar, M. Kašný: Molecular characterization of cystatins of Trichinella spp. BSc/MSc 10.30 - 11.00 Coffee break Session VI Peptidases and inhibitors (Chairman: L. Mikeš) 11.00 - 11.15 J. Ilgová, M. Gelnar, M. Kašný: Expression of a cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea). PhD 11.15 - 11.30 L. Jedličková, H. Dvořáková, M. Kašný, D. Potěšil, Z. Zdráhal, L. Mikeš: Peptidases detected in excretory/secretory products of the monogenean Eudiplozoon nipponicum. PhD 11.30 - 11.45 K. Skipalová, L. Jedličková, H. Dvořáková, P. Brož, M. Kašný, L. Mikeš: Anticoagulation factors and blood uptake by monogeneans of the family Diplozoidae. BSc/MSc 11.45 - 12.00 T. Perháčová, L. Mikeš: Cathepsin L-like peptidases in cercariae of Diplostomum pseudospathaceum. BSc/MSc 12.00 - 12.15 A. Leontovyč, L. Ulrychová, C. R. Caffrey, A. J. O'Donoghue, M. Mareš, M. Horn, J. Dvořák: Trypsin loaded worms: SmSP2, predominant serine protease in S. mansoni mammalian stages. PhD 12.15 - 12.30 P. Fajtová, S. Štefanić, M. Hradilek, J. Dvořák, J. Vondrášek, A. Jílková, L. Ulrychová, J. H. McKerrow, C. R. Caffrey, M. Mareš, M. Horn: Prolyl oligopeptidase from the blood fluke Schistosoma mansoni: from functional analysis to anti-schistosomal inhibitors. PhD 12.30 - 12.45 A. Jílková, M. Horn, P. Řezáčová, J. Brynda, J. H. McKerrow, C. R. Caffrey, M. Mareš: Structural and functional analysis of the Schistosoma mansoni cathepsin B1 drug target. 13.00 - 13.30 Lunch 14.00 - 18.00 Trip to the Regent brewery/ Třeboň castle 18.00 - 18.30 Dinner 19.00 - 00.00 Free evening Programme THURSDAY, MAY 7 08.00 - 09.00 Breakfast 09.00 - 09.30 Invited talk: I. Schabussova: Immunomodulation by Parasites: The big comeback? (Chairman: I. Vokřál) Session VII Immunology and diagnostics (Chairman: I. Vokřál) 09.30 - 09.45 K. Melounová, J. Bulantová, J. Vadlejch, P. Horák, M. Kašný: Evaluation of qualitative and quantitative parametrs of interaction between Fascioloides magna. and definitive experimental host - rabbit. BSc/MSc 09.45 - 10.00 J. Novák, L. Panská, L. Kolářová, P. Horák: Immunological consequences of the infection of mice with Toxocara canis. PhD 10.00 - 10.15 N. Reslová, M. Kašný, M. Slaný, B. Koudela, P. Králík: Novel molecular diagnostic approach for detection of pathogens in meat. PhD 10.15 - 10.30 L. Turjanicová, L. Mikeš: Enolase: a suitable antigen for the immunodiagnostics of Trichobilharzia regenti infections in ducks. PhD 10.30 - 10.45 M. Vaščiková, L. Turjanicová, P. Horák: Molecular and serological diagnosis of infections caused by Trichobilharzia spp. BSc/MSc 10.45–11.15 Coffee break Session VIII Development and physiology of helmints (Chairman: V. Skála) 11.15 - 11.30 J. Pankrác, M. Kašný, J. Bulantová, P. Horák: The role of stem cells in regenerative processes in trematodes. PhD 11.30 - 11.45 K. Vrbová, J. Pankrác, M. Kašný: In vitro cultivation of the trematode species Fascioloides magna and Trichobilharzia regenti. BSc/MSc 11.45 - 12.00 J. Peštová, P. Horák: Differentiation of totipotent germinal cells in larvae of T. regenti. BSc/MSc 12.00 - 12.15 I. Vokřál, Z. Seifertová, M. Veverková, L. Stuchlíková, L. Prchal, B. Szotáková, L. Skálová: Possible defence mechanisms against treatment in tapeworms.