Anti-DICER1 monoclonal antibody, clone BDN5 (DCABH-6086) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Rat monoclonal to Dicer - BSA and Azide free Antigen Description Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. Immunogen Synthetic peptide corresponding to Human Dicer. Isotype IgG Source/Host Rat Species Reactivity Human Clone BDN5 Purity Immunogen affinity purified Conjugate Unconjugated Applications IHC-P, WB Positive Control This antibody gave a positive signal in the following whole cell lysates: HepG2; Caco2; HeK293. Format Liquid Size 100 μg Buffer pH: 7.4; Constituent: PBS Preservative None Storage Store at -20°C or -80°C. Avoid freeze / thaw cycle. Ship Shipped at 4°C. 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved GENE INFORMATION Gene Name DICER1 dicer 1, ribonuclease type III [ Homo sapiens ] Official Symbol DICER1 Synonyms DICER1; dicer 1, ribonuclease type III; Dicer1, Dcr 1 homolog (Drosophila); endoribonuclease Dicer; Dicer; dicer 1; double stranded RNA specific endoribonuclease; HERNA; K12H4.8 LIKE; KIAA0928; K12H4.8-LIKE; helicase MOI; helicase-moi; Dicer1, Dcr-1 homol Entrez Gene ID 23405 Protein Refseq NP_001182502 UniProt ID Q9UPY3 Chromosome Location 14q32.2 Pathway Gene Expression, organism-specific biosystem; MicroRNA (miRNA) Biogenesis, organism- specific biosystem; Regulatory RNA pathways, organism-specific biosystem; Small Interfering RNA (siRNA) Biogenesis, organism-specific biosystem; mRNA processing, organism-specific biosystem; Function ATP binding; ATP-dependent helicase activity; double-stranded RNA binding; endonuclease activity; helicase activity; hydrolase activity; metal ion binding; miRNA binding; nucleotide binding; protein binding; ribonuclease III activity; ribonuclease III act 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.