Anales del Jardín Botánico de Madrid ISSN: 0211-1322 [email protected] Consejo Superior de Investigaciones Científicas España Dueñas, Margarita; Telleria, M. Teresa; Melo, Ireneia; Martín, María P. Lagarobasidium calongei (Aphyllophorales, Basidiomycota), a new species of corticioid fungi from Azores Islands Anales del Jardín Botánico de Madrid, vol. 66, núm. 1, 2009, pp. 41-46 Consejo Superior de Investigaciones Científicas Madrid, España Available in: http://www.redalyc.org/articulo.oa?id=55612935004 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative 01 primeras:01 primeras.qxd 10/12/2009 13:04 Página 1 Volumen 66S1 (extraordinario) 2009 Madrid (España) ISSN: 0211-1322 En homenaje a Francisco DE DIEGO CALONGE CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS lagarobasidium:hydnum.qxd 10/12/2009 13:11 Página 41 Anales del Jardín Botánico de Madrid Vol. 66S1: 41-46, 2009 ISSN: 0211-1322 doi: 10.3989/ajbm.2230 Lagarobasidium calongei (Aphyllophorales, Basidiomycota), a new species of corticioid fungi from Azores Islands by Margarita Dueñas1a, M. Teresa Telleria1b, Ireneia Melo2 & María P. Martín1c 1 Real Jardín Botánico, CSIC, Plaza de Murillo 2, E-28014 Madrid, Spain [email protected] 1a, [email protected] 1b, [email protected] 1c 2 Jardim Botânico (MNHN), Universidade de Lisboa/CBA-FCUL, Rua da Escola Politécnica 58, 1250-102 Lisboa, Portugal [email protected] Abstract Resumen Dueñas, M. Telleria, M.T., Melo, I. & Martín, M.P. 2009. Lagaro- Dueñas, M. Telleria, M.T., Melo, I. & Martín, M.P. 2009. Lagaro- basidium calongei (Aphyllophorales, Basidiomycota), a new basidium calongei (Aphyllophorales, Basidiomycota), una nueva species of corticioid fungi from Azores Islands. Anales Jard. Bot. especie de las Islas Azores. Anales Jard. Bot. Madrid 66S1: 41-46 Madrid 66S1: 41-46. (en inglés). On the base of morphologic and molecular studies, a new Sobre la base de estudios morfológicos y moleculares se descri- species of the genus Lagarobasidium (Aphyllophorales, Basi- be e ilustra una nueva especie dentro del género Lagarobasi- diomycota), is described and illustrated. This new species was dium (Aphyllophorales, Basidiomycota). Esta nueva especie, re- collected on Pico Island (Azores) and is characterized by having colectada en la isla de Pico (Azores), se caracteriza por presentar two types of cystidia, clavate leptocystidia and cylindrical skele- dos tipos de cistidios, leptocistidios claviformes y esqueletocisti- tocystidia, and smooth, thick-walled spores. It is compared with dios cilíndricos, y esporas lisas con paredes gruesas. Se compara Lagarobasidium detriticum (Bourdot) Jülich and L. cymosum con Lagarobasidium detriticum (Bourdot) Jülich y L. cymosum (D.P. Rogers & H.S. Jacks.) Jülich, until now the only two species (D.P. Rogers & H.S. Jacks.) Jülich, las otras dos especies del of the genus. género. Keywords: Corticiaceae, mycobiota, taxonomy, Atlantic Is- Palabras clave: corticiáceos, micobiota, islas atlánticas, Ma- lands, Macaronesia. caronesia. Introduction Later, Jülich (1979) transferred H. detriticum to the genus Lagarobasidium, L. detriticum (Bourdot) Jülich, The genus Lagarobasidium was described by Jülich due to its striking microscopic characteristics, clavate (1974) to include corticioid species with “basidiocarp cystidia and thick-walled cyanophilous spores. The effused, hymenial surface even or odontoid, hyphal species described here was collected from one locality system monomitic, cystidia or cystidiols present, ba- in Pico Island (Azores archipelago) and shows close sidia smaller than 20 µm and spores smooth, ellip- affinity to L. detriticum, although differing in that it soidal, thick-walled, often guttulate, not amyloid”. He has two types of cystidia. included three species in the genus: Lagarobasidium There are different opinions about the taxonomic pruinosum (Bres.) Jülich and L. nikolajevae (Parmas- position of L. detriticum –Langer (1994), for instance, to) Jülich, considered synonymous of Hypochnicium included it in Hyphodontia– but the molecular analy- detriticum (Bourdot) J. Erikss. & Ryvarden by Eriks- sis of samples deposited in MA herbarium shows that son & Ryvarden (1976) and L. cymosum (D.P. Rogers it belongs to a genus different from Hyphodontia and & H.S. Jacks.) Jülich. Hypochnicium. lagarobasidium:hydnum.qxd 10/12/2009 13:11 Página 42 42 M. Dueñas & al. Materials and methods Taxonomic treatment The sample under study was collected on Pico Is- Lagarobasidium calongei M. Dueñas, Telleria, Melo land, during a mycological foray to the islands from & M.P. Martín, sp. nov. (Figs. 1, 2, 3) the central group of Azores archipelago at the end of MycoBank N.º: MB 51283. the winter of 2005, on a Laurus azorica (Seub.) Franco Etymology: Dedicated to F.D. Calonge. remnant plot, accompanied by Erica azorica Hochst. ex Seub., Myrica faya Aiton, Picconia azorica (Tutin) Fructificatio resupinata, tenuis, colliculosa, ab alba Knobl., Pittosporum undulatum Vent, Dryopteris ad albam-luteam, margo macrescens. crispifolia H. Rasbach & al., Dryopteris affinis (Lowe) Systema hypharum monomiticum, hyphae fibulatae, Fraser-Jenkins, Hedera azorica Hort. and Rubia agos - tenuiter tunicatae; skeletocystidia cylindrica 140-160 × tinhoi Dansereau & P. Silva. The material was de- 5-9 mm, leptocystidia a subclaviformis ad spathuli- posited in the herbarium MA under MA-Fungi formia 57-90 × 6-7 mm; basidia a subcylindrica ad ur- 73256. The initials Tell. correspond to M.T. Telleria. niformia 17-28 × 4-6 mm; sporae a laxe ellipticis ad Collections of Lagarobasidium detriticum located subglobosas 7-8 × 5-6 mm, cum parietibus crassis et le - at the MA herbarium (MA-Fungi 5758, MA-Fungi vibus, non amyloideae et cyanophylae. Haec species dif- 61397 and MA-Fungi 61402) were included for com- fert ab alteris eiusdem generis per duos typos cysti - parison. diorum et per dimensionem sporarum. All material was studied following classical meth- Holotypus: PORTUGAL. Azores: Pico, S. Roque, ods for the corticioid fungi: thin, freehand sections Meia Encosta de Sta. Luzia, Travessa de Cima, from the specimen were mounted in KOH (5%) 26SLH8064, 26-II-2005, 483 m, on decayed wood, and/or Melzer’s reagent. These sections were exam- 16187Tell. (MA-Fungi 73256). ined under an Olympus BH50 microscope. Line drawings and microphotographs were made with a Basidiome resupinate, effused, hypochnoid, very Leyca DM 2500 microscope with camera lucida, by thin at first, white to white-yellowish; hymenophore M. Dueñas. when young smooth, with age colliculose to grandin- Genomic DNA was extracted from the four collec- ioid; margin not especially differentiated. tions using less that 10 mg of basidiomes with Hyphal system monomitic; hyphae distinct, thin- E.Z.N.A.-Fungi DNA miniprep kit (Omega Biotek, walled, fibulate, basal hyphae 2.5-3 µm wide, richly Doraville, USA). DNA fragments containing internal branched, forming a thin subiculum that gives rise transcribed spacers ITS1 and ITS2 including 5.8S to subhymenial hyphae arranged perpendicularly, nrDNA, were amplified with primer pair ITS1/ITS4 3-4 µm wide, densely united in the more developed (White & al., 1990) primers as described in Martín & zone. Cystidia of two kinds, skeletocystidia that rise al. (2004). Prior to sequencing, the amplification from the subiculum, projecting through the hymeni- products were cleaned using QIAquick Gel PCR pu- um, cylindrical, with very thick walls except in the api- rification kit (QIAGEN, Valencia, California, USA). cal part, cyanophilous, with secondary septa without Both strands were sequenced separately using pri - clamps, 140-160 × (5)7-9 µm and leptocystidia origi- mers ITS1 and ITS4 with AmpliTaq DNA Poly- nated from the subhymenial hyphae, subclaviform to merase (Applied Biosystem) at Secugen Company spathuliform, thin-walled, 57-90 × 6-7 µm (width on (Madrid, Spain). the apical part); frequently capitate cystidiols. Basidia Sequencher (Gene Codes Corporation, Ann Ar- subcylindrical to urniform with constrictions, 17-28 × bor, Michigan, USA) was used to identify the consen- 4-6 µm, with 4 sterigmata. Basidiospores broadly sus sequence from the two strands of the ITS nrDNA ellip soid to subglobose, smooth, thick-walled, of each isolate. Basic Local Alignment Search Tool cyanophilous, non-amyloid, 7-8 × 5-6 µm, with one oil (BLAST) with option Standard nucleotide BLAST of drop and a small apiculus. BLASTN 2.2.18 were used to compare the sequence Molecular analysis: ITS nrDNA sequences were obtained against the sequences in the National Center obtained from a single L. detriticum collection (MA- of Biotechnology Information (NCBI) nucleotide Fungi 5758) and L. calongei (MA-Fungi 73256). The databases (Altschul & al., 1997). The new consensus new sequences have been logged in the EMBL data- sequences have been deposited in the EMLB data- base with the Accession Numbers FM876211 and base. SEQAPP software for multiple sequences was FM876212, respectively. The alignment between used to compare the sequences obtained in this study. these sequences is shown in Fig. 3. The ITS1 re- Based on morphological and molecular data a new gion has at least 68 different bases, of which 42 are species is proposed: due to insertions, 11 to transitions (indicated with !, Anales del Jardín Botánico de Madrid 66S1: 41-46, 2009. ISSN: 0211-1322.