Project ID: GBPNI/NMHS-2017-18/MG-21 ANNUAL PROGRESS REPORT OF THE PROJECT ON Assessment of Environmental Health and Ichthyofaunal Biodiversity of Tirap and Tissa rivers of Arunachal Pradesh and promotion of Fish centric supplementary livelihood options through a participatory approach Submitted to: Nodal Officer, NMHS-PMU National Mission on Himalayan Studies (NMHS) G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263643, Uttarakhand Submitted by: Dr. Rajdeep Dutta Department of Aquatic Environment Management, College of Fisheries, Assam Agricultural University, Raha, Nagaon-782 103 NMHS Progress Report (Period from April, 2018 to January, 2019) 1. Project Information Project ID: GBPNI/NMHS-2017-18/MG-21 Sanction Date: 26-02-2018 Assessment of Environmental Health and Ichthyofaunal Biodiversity of Tirap Project Title: and Tissa rivers of Arunachal Pradesh and promotion of Fish centric supplementary livelihood options through a participatory approach BTG: Conservation and Sustainable Use of Biodiversity PI and Dr. Rajdeep Dutta Affiliation Assistant Professor, Department of Aquatic Environment Management, (Institution): College of Fisheries, Assam Agricultural University, Raha, Nagaon-782 103 Dr. Sarada Kanta Bhagabati, Asst. Prof., Deptt. of AEM, College of Fisheries, AAU, Raha, Nagaon Dr. Krishna Kanta Tamuli, Professor, Deptt. of Aquaculture, College of Fisheries, AAU, Raha, Nagaon Name & Dr.Binod Kalita, Professor & Head, Deptt. of Aquaculture, College of Fisheries, Address AAU, Raha, Nagaon of the Co-PI: Mr.Phurin Songtheng, Subject Matter Specialist (Fisheries), Krishi Vigyan Kendra, Tirap district, Deptt. of Agriculture, Govt. of Arunachal Pradesh, PO- Deomali, Arunachal Pradesh, Pin- 792129 Structured Recruitment of Project Staff: Two Junior Project Fellows (JPFs) were recruited Abstract- for carrying out the project activities in the month of May, 2018. detailing the Procurements: Procurement of Equipments, Chemicals, Glasswares, current year Plasticwares etc. are completed complying all necessary procedures. progress Initial Survey: As per the Sanction letter of the project, a preliminary survey of river Tirap and Tissa was carried out in the month of June, 2018 for preparation of a baseline data report . The report was submitted to NMHS PMU. Project Launch Workshop: All the stakeholders, co-PIs attended the launch workshop of the project held on 9th July, 2018 at College of Fisheries, AAU, Raha. A souvenir was also released on this occasion. Assessment of ichthyofaunal diversity & molecular characterization: To study fish faunal diversity of river Tirap and Tissa, fish species collection was carried out from 5 sampling sites each of both the rivers in alternate months. A total number of 25 species have been collected so far from the selected sampling sites of river Tissa and Tirap. Of these,7 fish species collected from River Tissa are identified using standard keys and further confirmed with the help of DNA barcoding technique. Mitochondrial COI gene sequences of 34 number of fish specimens from river Tissa were successfully submitted to NCBI. Identification and DNA barcoding work of fish specimens from river Tirap is going on. Assessment of Environmental Health: Assessment of environmental health of river Tirap and Tissa is going on based on periodic monitoring of physico- chemical parameters (Temperature, Turbidity, pH, Water Velocity, DO, COD, Total Alkalinity, Total Hardness, TDS, Conductivity, Nutrients ) of river water samples collected from 5 sampling sites each of both the rivers. Project Partner Affiliations Role & Responsibilities Name Dr. Sarada Kanta Bhagabati Asst. Prof., Deptt. of AEM, Ichthyofaunal diversity assessment, College of Fisheries, AAU, Initial processing of DNA samples & Raha, Nagaon Evaluation of Environmental health of streams Dr. Krishna Kanta Tamuli Professor, Deptt. of Development of model aquaculture Aquaculture, College of systems as proposed in the project Fisheries, AAU, Raha, Nagaon Dr.Binod Kalita Dr.Binod Kalita, Professor & Development of model aquaculture Head, Deptt. of Aquaculture, systems, training & demonstration. College of Fisheries, AAU, Raha, Nagaon Mr.Phurin Songtheng Subject Matter Specialist Sample collection, field surveys and (Fisheries), Krishi Vigyan local level management of the Kendra, Tirap district, Deptt. of project. Agriculture, Govt. of Arunachal Pradesh, PO- Deomali, Arunachal Pradesh, Pin- 792129 2. Project Site Details River Tirap Project Site Barap, Near Lazu Circle, Changlang Town Proper, Changlang Town Outskirts Tirap District Changlang District (Hanging Bridge point), Changlang District IHR state Arunachal Pradesh Arunachal Pradesh Arunachal Pradesh covered Long. & lat. 26⁰52/35//N 27°8´10ʺ N 27°8´22ʺ N 95⁰31/36//E 95°44´14ʺ E 95°44´40ʺ E Elevation:349m above MSL Elevation:1100m above Elevation: 332m above MSL MSL Site Photograph Project Site Jairampur, Changlang District Old Khamdu Village, Changlang District IHR state Arunachal Pradesh Arunachal Pradesh covered Long. & lat. 27°21´3.99ʺ N 27019.7070´N 96°0´56ʺ E 95058.3920´ E Elevation: 191 m above MSL Elevation: 175 m above MSL Site Photograph River Tissa Project Site Tissa bridge point, Tupi village point, Nakfan village, Longding Longding district Tirap district district IHR state Arunachal Pradesh Arunachal Pradesh Arunachal Pradesh covered Long. & lat. 26°54·5´ N 26°97´ N 27⁰5/N 95°21·8´ E 95°47´ E 95⁰34/E Elevation 750 m above Elevation 625 m above MSL MSL Elevation 162m above MSL Site Photograph Project Site Naitong village, Otongkhua village, Longding district Longding district IHR state covered Arunachal Pradesh Arunachal Pradesh Long. & lat. 27°03´26.7ʺ N 27°5.821´N 95°20´ 31ʺE 95°22.195´E Elevation 150 m above Elevation 150 m above MSL MSL Site Photograph 3. Project Activities Chart w.r.t. Timeframe [Gantt or PERT] Project Work undertaken Output activities Year 2018-2019 Project Qtr-1 Qtr-2 Qtr-3 Qtr- Activity 1 4 JPFs recruited Project launch Routine sampling of workshop the project continued A souvenir of organized at for the months of Project Launch College of October, November Workshop Fisheries, AAU, and December, published Raha on 9th 2018 July, 2018 Project Procurements Most of the Assessment of Lab set up Activity 2 carried out. instruments of Environmental completed and the project health of Tirap & protocols were installed and Tissa rivers standardized routine continued for the sampling of the months of October, project initiated November and from the month December, 2018. of September, 2018. Project Baseline survey Initial taxonomic 5 sampling Activity 3 of the rivers identification of stations each carried out in the collected from both the fish specimens the month of river selected. initiated. June, 2018 Preliminary Baseline survey DNA barcoding report. work started. Seven fish species collected from River Tissa identified using standard keys and were further confirmed with the help of DNA barcoding technique. The sequences were successfully submitted to NCBI and accession number acquired. Project Environmental - Activity 4 health assessment of the rivers under study initiated. 4. Financial and Resource Information Note: A separate bank account is expected to be opened for NMHS Project as per the provision of Direct Beneficiary Account (DBA) as laid out by the Govt. of India and also facilitate the audit of accounts. The interest earned out of the NMHS project funds should be reported clearly in the utilization certificate. Total Grant: 65,00,000.00 Grant Received Date: 19-04-2018 Project Affiliations/ Institution Budget Allocated to Work Done Partner(s) College of Fisheries, Lead Assam Agricultural Assam Agricultural As per project plan Institute University,Raha ,Nagaon University Assam Project Staff Information: S. No. Name Qualification Designation Fellowship/ Wages paid Remarks Dibyojyoti M.Sc. (Life 1. JPF 16000.00 Baruah Science) Deepjyoti 2. M.F.Sc. JPF 16000.00 Bora 5. Equipment and Asset Information Sl. Equipment Name Details (Make/ Cost Date of Photographs Lowest No. (Qty) Model) Installation of Quotation, Equipment IF NOT purchased 1. Nano drop Genetix Brand 4,83,000.00 7/06/2018 Spectrophotometer Micridigital (One) Company/NABI UV/ Vis Nano Spectrophotometer 2. COD digester KEL PLUS 88,796.00 13/06/2018 (One) Automatic COD Digestion system//KES 08 L CAC Attached in Does not 3. Digital soil & water Systronics (India) 82,600.00 13/06/2018 Annexure IV arise testing kit (One) Limited/371 4. Current meter Raj Instrument 59,590.00 22/06/2018 (One) 5. Gradient Thermal Eppendrof Gradient 2,73,000.00 22/06/2018 cycler (One) Thermal Cycler/MasterCycler Nexus Gradient 6. Expenditure Statement and Utilization Certificate Please update the annual Expenditure Statement and Utilization Certificate (UC) periodically. Expenditure Information: S. No. Financial Position/Budget Head Funds Sanctioned Expenditure % of Total cost I Salary 3,84,000.00 2,65,802.00 69.22 II Travel( Domestic TA/DA) 1,75,000.00 1,51,955.00 86.83 III Consumables 4,00,000.00 3,88,398.00 97.09 IV Contingency 1,00,000.00 60,000.00 60 V Activities & Other Project cost 1,00,000.00 1,00,000.00 100 VI Institutional Charges - - - VII Equipments 12,73,520.00 11,01,563.00 86.49 24,32,520.00 20,67,718.00 85.00 Interest earned Grand Total 8. Project Progress Summary (as applicable to the project) Description Total (Numeric) Description IHR States Covered 01 Arunachal Pradesh Photos & Maps attached Photographs of Sampling sites and map of study area attached