Table Indexof Contents Column Selection 1 SPE Phase Selection ...........................................................2 HPLC and LC/MS Column Selection...................................4 GC Column Selection ........................................................17 SPE 29 HyperSep SPE Products ....................................................30 Finnpipettes.......................................................................46 GC and GC/MS 47 TRACE GC Capillary Columns ...........................................48 Ultra Fast GC Columns......................................................65 HPLC and LC/MS 65 Column Protection.............................................................66 Hypersil GOLD Columns....................................................70 BioBasic Columns..............................................................88 Hypercarb Columns.........................................................101 Hypersil BDS Columns ....................................................106 Hypersil Classical Columns.............................................111 Polymeric HPLC Columns ................................................122 AQUASIL C18 Columns ...................................................128 BetaBasic Columns .........................................................129 BetaMax Columns...........................................................130 BETASIL Columns............................................................130 Other HPLC Columns.......................................................132 Accessories for HPLC and GC 135 HPLC Accessories............................................................136 HPLC Injectors and Valves ..............................................144 GC Accessories ...............................................................150 Syringes...........................................................................161 Vials, Caps and Vial Accessories....................................169 Chromatography and Mass Spectrometry Instrument Parts and Supplies 175 GC and GC/MS................................................................176 Turboflow Columns..........................................................188 LC and LC/MS .................................................................189 MS ...............................................................................195 IRMS ...............................................................................198 ICP-MS ............................................................................199 Technical Information 203 SPE Method Development and Troubleshooting............204 HPLC Method Selection, Troubleshooting and Reference Data...............................................207 GC Method Selection, Troubleshooting and Reference Data...............................................221 Applications 229 Pharma/Biochem.............................................................230 Environmental .................................................................258 Forensics/Toxicology.......................................................276 Industrial .........................................................................288 Food Safety .....................................................................302 Indexes 317 Applications Index...........................................................318 Alphabetical Index ..........................................................330 i Trademark Information Trademark Information The Thermo Scientific name, the Thermo Scientific logo and the following trademarks are the property of Thermo Fisher Scientific Inc. and/or its subsidiaries: BetaBasic Dual Desolvation Zone HyperPrep LTQ Surveyor MSQ Plus BetaMax Evolution HyperQuad LTQ FT Surveyor Plus BETASIL Fingerlok HyperREZ LXQ TRACE BioBasic Finnigan HyperSep MSQ TRACE GC Ultra BioMate Fluophase HyPURITY Open Access Transcend BioWorks FocusLiner HyPURITY ADVANCE PRISM TriPlus ChromQuest FOCUS HyPURITY AQUASTAR ProteomeX TSQ Quantum COOL POCKET HOT POCKET Ion Bright Retain TSQ Quantum Ultra DASH Hypercarb Javelin SLIPFREE UNIGUARD Deca HyperGEL KAPPA Smart Accessories Verify DELTABOND Hypersil LCQ SpectraSYSTEM Watson LIMS Discovery Hypersil GOLD LCQ Advantage Spectronic Xcalibur DSQ Hypersil GOLD aQ LightPipe Surveyor The following brands, trademarks or service marks are the property of the listed company and/or its subsidiaries. Every effort has been taken to ensure this list is accurate at the time of printing this catalog. 3M Company Macherey-Nagel GmbH Sigma-Aldrich Corporation Kel-F CHROMABOND, NUCLEODUR, Nucleosil, Optima Discovery, Meridian MDN, Nukol, Petrocol, PTE, SP, Agilent Technologies, Inc. Mallinckrodt Baker, Inc. SPB, SPME, Supelclean, Supelcosil, SUPELCOWAX, AccuBOND, Agilent, DB, Evidex, HP-INNOWax, BAKERBOND spe, BAKERBOND, Polar Plus VOCOL HP-Ultra, Zorbax Merck KGaA Tosoh Corporation Applera Corporation LiChrosorb, LiChrospher, Purospher TSKgel POROS Merlin Instrument Company Union Carbide Corporation Becton, Dickinson and Company Microseal Carbowax Luer-LOK Neos Corporation United Chemical Technologies, Inc. Bio-Rad Laboratories, Inc. Fluofix Clean-Up Aminex New Objective, Inc. University of Washington Biotage AB IntegraFrit, PicoFrit SEQUEST, TurboSEQUEST EVOLUTE, Isolute Ohio Valley Specialty Chemical Company Upchurch Scientific, Inc. Bristol-Myers Squibb Company OV Bottom-of-the-Bottle, Upchurch Scientific Taxol Parker Hannifin Corporation Varian, Inc. Capital Analytical, Ltd. Parker Bond Elut, Bond Elut Certify, CB, CC, CP-Select, CP-Sil, CP-Wax, Polaris, Pursuit, Varian RH PerkinElmer, Inc. Chromatography Research Supplies, Inc. Clarus , PE Velcro Industries B.V. Velcro BTO Pfizer, Inc. Cohesive Technologies, Inc. Dostinex VICI, Valco Instruments Co., Inc. Cheminert, Valco Aria Phenomenex, Inc. Dow Chemical Company, The AQUA, Columbus, Gemini, Jupiter, Luna, Prodigy, Victrex plc. TRITON X-100 strata, Synergi, Ultracarb, Zebron ZB VICTREX, PEEK E.I. Dupont de Nemours and Company Porvair plc Waters Corporation Teflon, Tefzel, Vespel, Viton Porvair µBondapak, ACQUITY UPLC, Atlantis, Nova-Pak, Sep-Pak, Styragel, SunFire, Symmetry, Ultrahydrogel, Eka Chemicals AB Quadrex Corporation Ultrastyragel, Waters, µBondapak, Waters Spherisorb, Kromasil 007 Symmetry, SymmetryShield, XBridge, Xterra Floridin Company Restek Corporation Whatman, Inc. Florisil Rtx, Stabilwax, Pinnacle, Viva Partisil General Electric Company Rheodyne LLC W.R. Grace & Company SE Make-Before-Break (MBB), RheBuild, RheFlex, Alltima, AT, Denali, Genesis, Vydac Rheodyne GL Sciences, Inc. YMC Co., Ltd. Inertsil Scientific Glass Technology Exploitatie B.V. ODS-AQ, ODS-A, Pro-C18, YMCbasic, YMC-Pack, Hamilton Company CLICK-ON, Shortix, SGT, Super Clean YMC Hamilton SGE, Inc. Other Notes: HighChem, Ltd. Auto-Sep T, Auto-Sep, BP, BPX, Enduro, SGE, Mass Frontier PEEKsil, SilTite, Terry Tool AquaSil™ Siliconizing Fluid for treating glass surfaces is sold by Pierce Chemical Co., Rockford, IL Hewlett-Packard Development Company Shimadzu Corporation HP Shimadzu Kimberly-Clark Corporation Showa Denko K.K. Kimwipes Asahipak, Shodex ii To order or for customer support, please see back cover. GC and GC/MS GC and GC/MS Thermo Scientific TRACE™ GC Columns offer high temperature stability and exhibit low bleed and long lifetimes. From general purpose non-polar to polar columns, many of which are MS-compatible, TRACE GC columns provide excellent quality and performance, with guaranteed reproducibility. TRACE GC Columns Thermo Scientific TRACE GC Capillary Columns A leap forward in column performance • Low bleed even at elevated Example of TR-WaxMS robustness. temperatures Top: New column. • Outstanding robustness for difficult separations • Reliable and reproducible performance • Complete range of GC consumables for all instruments Bottom: Column performance after being heated to 250 °C for 1 hour with no carrier gas. Although a loss of retention and sensitivity can be seen, the column shows extraordinary robustness after this treatment. Reproducibility As well as focusing on individual column The heart of any gas chromatograph is the performance, we work to improve column-to- column. We have used the knowledge column reproducibility, arising from major gained over 30 years of HPLC column improvements in the production processes. production and 50 years in GC and GC/MS A column bought today will have the same instrument manufacturing to offer a range characteristics or better as one obtained a of columns and consumables that are year or ten years from now. unsurpassed in the most important aspects of their performance. Guaranteed Performance All TRACE columns have passed rigorous Low Bleed testing procedures to ensure the highest The stability of a TR-5MS column after three days Bleeding phases cause problems including possible performance and are delivered with a at 200 °C using air as a carrier gas. Very little change in the chromatography is evident below serialized certificate to show their performance low sensitivity, detector contamination, lower the column’s maximum operating temperature. temperature limits and short lifetimes. All and assist with traceability. All Thermo manufacturers have at some time claimed Scientific columns come with a 100% guar- Quick Reference Icon antee. If for any reason the column does not their columns have the lowest bleed, but use The reference graphic (below) on each perform up to our claims, we will replace it supporting chromatograms that lack repro- column page gives easy access to the with one that does. ducible