A New Species of the Hypsiboas pulchellus Group from the Serra da Mantiqueira, Southeastern Brazil (Amphibia: Anura: Hylidae) Author(s): Paulo D.P. Pinheiro , Tiago L. Pezzuti , Felipe S.F. Leite , Paulo C.A. Garcia , Célio F.B. Haddad , and Julián Faivovich Source: Herpetologica, 72(3):256-270. Published By: The Herpetologists' League DOI: http://dx.doi.org/10.1655/Herpetologica-D-15-00062.1 URL: http://www.bioone.org/doi/full/10.1655/Herpetologica-D-15-00062.1 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. Herpetologica, 72(3), 2016, 256–270 Ó 2016 by The Herpetologists’ League, Inc. A New Species of the Hypsiboas pulchellus Group from the Serra da Mantiqueira, Southeastern Brazil (Amphibia: Anura: Hylidae) 1 2 3 2 1 PAULO D.P. PINHEIRO ,TIAGO L. PEZZUTI ,FELIPE S.F. LEITE ,PAULO C.A. GARCIA ,CE´ LIO F.B. HADDAD , AND JULIA´ N 4,5,6 FAIVOVICH 1 Laboratorio´ de Herpetologia, Departamento de Zoologia, Instituto de Biociencias,ˆ Universidade Estadual Paulista, Rio Claro, SP, Brazil 2 Laboratorio´ de Herpetologia, Departamento de Zoologia, Instituto de Cienciasˆ Biologicas,´ Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil 3 Universidade Federal de Vi¸cosa, Campus Florestal, Florestal, Minas Gerais, Brazil 4 Division´ Herpetologıa,´ Museo Argentino de Ciencias Naturales ‘‘Bernardino Rivadavia’’—CONICET, Angel Gallardo 470, C1405DJR, Buenos Aires, Argentina 5 Departamento de Biodiversidad y Biologıa´ Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina ABSTRACT: A new species of the Hypsiboas pulchellus group is described from the Mantiqueira range, in the Municıpio´ de Rio Preto, State of Minas Gerais, Brazil. We describe adults, tadpoles, and the advertisement call. The new species is morphologically similar to H. freicanecae,a species known from a few localities in the states of Alagoas and Pernambuco, northeastern Brazil, ~1640 km north. Adults differ from H. freicanecae in having a slender body, smaller male size, larger calcar, and hidden surfaces of thighs and feet orange in life. Tadpoles have a ventral oral disc, with labial tooth row formula 2(2)/3–4(1), with one to three narrow posterior gaps on the marginal papillae located on the oral disc emarginations. The advertisement call is composed of two nonpulsed notes with dominant frequency at the second harmonic. The species is known only from its type locality, in an unprotected area. Key words: Cophomantini; Species description; Tadpoles; Taxonomy; Treefrogs; Vocalization THE HYPSIBOAS pulchellus group is the most species rich of MATERIALS AND METHODS the seven species groups of Hypsiboas Wagler 1830 Adult Morphology and Species Description (Faivovich et al. 2005). The group currently includes 38 species, representing more than one-third of the described Adults were euthanized in 5% lidocaine, fixed in 10% species in the genus (91 spp.; Frost 2016). The monophyly of formalin, and preserved in 70% ethanol. Voucher speci- the group has been corroborated by several analyses on the mens are housed in the Celio F.B. Haddad Amphibian basis of molecular data (Faivovich et al. 2004, 2005, 2013; Collection, Rio Claro, Sa˜o Paulo, Brazil (CFBH); Amphib- Wiens et al. 2010; Pyron and Wiens 2011). The only putative ian Collection of Centro de Cole¸coes˜ Taxonomicasˆ da phenotypic synapomorphy known for the group so far is the Universidade Federal de Minas Gerais, Belo Horizonte, absence of the slip of the depressor mandibulae muscle of Minas Gerais, Brazil (UFMG); and Museu de Zoologia Joa˜o scapular origin (Faivovich et al. 2005). Moojen de Oliveira, Universidade Federal de Vi¸cosa, The species of the H. pulchellus group inhabit both Vi¸cosa, Minas Gerais, Brazil (MZUFV). Additional speci- streams and lentic water bodies in forests and grasslands, mens used for comparisons are listed in the Appendix; from northeastern Brazil in the State of Pernambuco to the museum acronyms follow Sabaj Pe´rez (2014). We measured pampean grasslands of Argentina, and westward to the specimens to the nearest 0.1 mm using digital calipers eastern slopes of the Andes, from northern Argentina to under a Zeiss Stemi SV 11 stereomicroscope. Measure- central Peru. Phylogenetic analyses of the group (Faivovich ments (in mm) follow Duellman (1970): snout–vent length et al. 2004, 2005, 2013; Kohler¨ et al. 2010; Lehr et al. 2010) (SVL), head length (HL), head width (HW), eye diameter show two Andean clades including four species each, whereas the bulk of the species included in the group are (ED), eye–nostril distance (END), internarial distance distributed in the Atlantic Forest in Brazil (20 species; only (IND), eyelid width (EW), interorbital distance (IOD), two reach adjacent areas in Argentina and Paraguay), seven tympanum diameter (TD), tibia length (TL), tarsal length species are distributed in the Cerrado formations, one mostly (TAL), and foot length (FL). We also measured snout in pampean grasslands, and one in the Central Sierras of length (SL; Cei 1980); thigh length (THL; Heyer et al. Argentina. 1990); finger III disc diameter (3FD), and toe IV disc During a field trip in the mountains of Serra Negra, part diameter (4TD; Napoli and Caramaschi 1999). Additionally, of the Serra da Mantiqueira (Mantiqueira mountain range) we measured calcar length (CAL; distance from the base to in the State of Minas Gerais, Brazil, we collected a new the tip of the calcar). Terminology for external morphology species of the H. puchellus group, morphologically similar to follows Duellman (1970), except for the dorsal outline of H. freicanecae (Carnaval and Peixoto 2004). In this paper, we the snout, which follows Heyer et al. (1990). Webbing describe its adult and larval morphology and its advertise- formulae follow Savage and Heyer (1967) as modified by ment call, and provide comments on the conservation status Myers and Duellman (1982). Sex was determined by the of Serra Negra. development of a prepollex, vocalization, or presence of vocal slits in males, and presence of mature oocytes in the 6 CORRESPONDENCE: e-mail, [email protected] female specimen. 256 PINHEIRO ET AL.—A NEW SPECIES OF HYPSIBOAS 257 Tadpoles TABLE 1.—Some measurements and proportions of the type series of Hypsiboas cambui. Values (mm) are reported as ranges (mean 6 1 SD). See Tadpoles were collected in the same pond where the text for explanation of measurement abbreviations. adults were collected, euthanized in 5% lidocaine solution, then preserved in 10% formalin (one specimen was Measurement Body ratios preserved in ethanol 95% as tissue for DNA analysis). Female Males Female Voucher lots are housed in the CFBH collection. To confirm Males (n ¼ 16) (n ¼ 1) (n ¼ 11) (n ¼ 1) the identity of the tadpoles, we used sequences of SVL 26.3–32.8 (30.3 6 1.7) 32.7 HW/HL 0.90–1.00 0.97 cytochrome oxidase c subunit I (primers AnF1 and AnR1; HL 9.3–11.3 (10.6 6 0.5) 11.8 HL/SVL 0.33–0.36 0.36 HW 9.2–10.8 (10.2 6 0.4) 11.5 HW/SVL 0.32–0.35 0.35 Jungfer et al. 2013; GenBank accessions KX241479— ED 3.2–3.9 (3.5 6 0.2) 3.5 EN/ED 0.69–0.89 0.90 KX241481 [tadpole, holotype, and recorded male paratype, EN 2.4–3.0 (2.8 6 0.2) 3.1 IOD/ED 0.75–1.09 0.84 respectively]) showing 100% similarity between adult and IND 1.8–2.1 (1.9 6 0.1) 2.1 IOD/HL 0.27–0.34 0.25 larva. Descriptions, measurements, and proportions of EW 2.1–3.0 (2.6 6 0.2) 2.8 IOD/HW 0.28–0.36 0.25 IOD 2.6–3.5 (3.1 6 0.2) 2.9 ED/HL 0.31–0.38 0.29 tadpole external morphology are based on six tadpoles in TD 1.3–1.7 (1.5 6 0.1) 1.8 ED/HW 0.32–0.39 0.30 Gosner’s (1960) stages 34–36 (lots CFBH 39392, 39405). To TL 14.0–16.8 (16.0 6 0.7) 16.9 EW/HL 0.20–0.27 0.24 assess ontogenetic variation, 23 specimens in stages 25–39 TAL 8.3–10.3 (9.4 6 0.5) 10.3 TD/3FD 0.88–1.26 1.20 were also analyzed (lots CFBH 39392, 39405). Measure- FL 11.6–14.1 (13.2 6 0.6) 14.0 TD/ED 0.38–0.47 0.53 ments and terminology follow Altig and McDiarmid (1999): SL 4.0–4.8 (4.5 6 0.2) 4.9 TD/HL 0.12–0.15 0.16 THL 12.7–15.9 (14.8 6 0.9) 15.9 TAL/SVL 0.29–0.33 0.31 total length (TL), body length (BL), tail length (TAL), 3FD 1.2–1.6 (1.4 6 0.1) 1.5 FL/SVL 0.42–0.45 0.43 maximum tail height (MTH), IND, IOD, tail muscle width 4TD 1.1–1.5 (1.3 6 0.1) 1.3 SL/HL 0.41–0.47 0.42 (TMW), and tail muscle height (TMH); Lavilla and Scrocchi CAL 0.6–1.1 (0.9 6 0.1) 1.1 SL/HW 0.42–0.48 0.43 (1986): body width (BW), body width at narial level (BWN), THL/SVL 0.46–0.52 0.48 TL/SVL 0.50–0.55 0.52 body width at eye level (BWE), body height (BH), eye–snout CAL/TAL 0.07–0.11 0.11 distance (ESD), END, nostril–snout distance (NSD), ED, 4TD/3FD 0.78–0.99 0.87 narial diameter (ND), snout–spiracular distance (SSD), and oral disc width (ODW).