University of New Hampshire University of New Hampshire Scholars' Repository Doctoral Dissertations Student Scholarship Fall 1984 OOGENESIS IN TUBULARIA LARYNX AND TUBULARIA INDIVISA (HYDROZOA, ATHECATA) BARRY W. SPRACKLIN University of New Hampshire, Durham Follow this and additional works at: https://scholars.unh.edu/dissertation Recommended Citation SPRACKLIN, BARRY W., "OOGENESIS IN TUBULARIA LARYNX AND TUBULARIA INDIVISA (HYDROZOA, ATHECATA)" (1984). Doctoral Dissertations. 1436. https://scholars.unh.edu/dissertation/1436 This Dissertation is brought to you for free and open access by the Student Scholarship at University of New Hampshire Scholars' Repository. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of University of New Hampshire Scholars' Repository. For more information, please contact [email protected]. INFORMATION TO USERS This reproduction was made from a copy of a document sent to us for microfilming. 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Zeeb Road Ann Arbor, Ml 48106 8428160 Spracklin, Barry W. OOGENESIS IN TUBULARIA LARYNX AND TUBULARIA INDIVISA (HYDROZOA, ATHECATA) University of New Hampshire Ph.D. 1984 University Microfilms International300 N. Zeeb Road, Ann Arbor, Ml 48106 PLEASE NOTE: In all cases this material has been filmed in the best possible way from the available copy. Problems encountered with this document have been identified here with a check markV . 1. Glossy photographs or pages^ 2. Colored illustrations, paper or print______ 3. Photographs with dark background 4. Illustrations are poor copy_______ 5. Pages with black marks, not original copy_______ 6. Print shows through as there is text on both sides of______ page 7. Indistinct, broken or small print on several pages ^ 8. Print exceeds margin requirements______ 9. Tightly bound copy with print lost in spine_______ 10. Computer printout pages with indistinct print______ 11. P age(s)_____________lacking when material received, and not available from school or author. 12. P age(s)_____________seem to be missing in numbering only as text follows. 13. Two pages numbered____________. Text follows. 14. Curling and wrinkled p a g e s _______ 15. Other______________________________________________________________________ University Microfilms International OOGENESIS IN TUBULARIA LARYNX AND TUBULARIA INDIVISA (HYDROZOA, ATHECATA) BY Barry W. Spracklin B.A., Noreastern University, 1969 M.S., Northeastern University, 1974 DISSERTATION Submitted to the University of New Hampshire in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Zoology Septem ber, 1984 This dissertation has been examined and approved. iJ a~ . c 3sertation direotor, John E. Foret ^sociate Professor of Zoology Larry G. H€rnis, Associate Professor of Zoology Nathan W. Riser, Professor of Marine Biology Marine Science and Maritime Studies Center Northeastern University . , , —7— r-mr- 5hn J. Saaner, Jr., Professor of Zoology Edward K. Tillinghast, Professor of Zoology 7! n If? Date ACKNOWLEDGEMENTS I am grateful for the support, assistance, and encouragement of my doctoral committee, Drs. John E. Foret, Larry G. Harris, John J. Sasner, Jr., Nathan W. Riser, and Edward K. Tillinghast. I thank Dr. Charles W. Walker who reviewed and commented on many parts of this dissertaion and for the use of his lab and research equipment. I am indebted to Eileen Wong for her excellent typing and also her patience. Thanks also to the many graduate students who have assisted in the collecting of specimens and who often returned from diving and collecting trips with hydroids. I gratefully acknowledge financial support of this research by the Central University Research Fund grant number S7^ and a research assistantship from Dr. Charles W. Walker and the Hubbard Farms Fund. I especially thank my family and friends for their encouragement and support during my graduate studies. i l l TABLE OF CONTENTS ACKNOWLEDGEMENTS............................................... i l l LIST OF F IG U R E S : .............................................................................................. ▼ ABSTRACT.................................................................................................................................. v i SECTION PAGE INTRODUCTION......................................................................................................................... 1 O ogenesis.......................................... 1 H ydroids ....................................................................... 4 Gonophores and Germ C e lls ............................................... ..................... 7 Microscopy and Autoradiography ...................................................................... 15 MATERIALS AND METHODS..................................................................................................... 21 Light Microscopy..................................................................................................... 21 A utoradiography........................................................................................................ 22 Electron Microscopy .............................................................................................. 24 RESULTS.................................................................................................................................... 26 Light Microscopy................................. 26 Autoradiography.......................................................................... .......................... 32 Electron Microscopy .............................................................................................. 76 DISCUSSION................................................. 112 LIST OF REFERENCES............................................................................................................ 136 iv LIST OF ILLUSTRATIONS PAGE 1. T. larvnx. Underwater photograph (20 feet) ........................................ 34 2. Clava leptostvla. Light microscopy photograph of gonophore.. 3b 3. 2. larvnx. Light microscopy photograph of small germ cell m ass............................................................................................ 38 4. 1. larynx. Light microscopy photograph of large germ cell m ass.............................................................................................................................. 40 5. T. larvnx. Light microscopy photograph of early stages of o o g en esis............................................................................................ 42 6. indivisa. Light microscopy photograph of developing o o c y te s............................................................ 44 7. £• larvnx. Light microscopy photograph of intercellular b rid g e s....................................................................................................................... 46 8. X. lndivisa. Light microscopy photograph of intercellular b rid g e s....................................................................................................................... 48 9. X* indivlsa. Light microscopy photograph of oocyte with two n u c le i .......................................................................................................................... 50 10. X . indivlsa. Light microscopy photograph of oocytes .................... 52 11. X* larvnx. Light microscopy photograph of an actinula .............. 54 12. X . indivisa. Light microscopy photograph of oocyte-nurse cell complex. ........................ 56 13. X« larynx. Light microscopy photograph of mature gonophore.. 58 14. X. indivi3a. Light microscopy photograph of oocyte