S635 Abstracts accepted for publication only body fluid (0.7%). In order to microbiologic studies all of samples Pathogenesis were cultured according to standard procedure. Some disks with especial antibiotics were chosen for each bacterium during the study of antibiotic R2126 The use of the PREVI™ Isola system in faecal and genital sensibility via disk diffusion agar (kirby bauer). Minimum inhibitory samples concentration via E-test method was used for especial antibiotic and E.A. Verhoef-Verhage °, A.P. van Boxtel (Utrecht, NL) finally compared two antibiogram methods together. Results: Most isolated bacterium was S. aureus (33.6%), Pseudomonas Introduction: This study is a follow up of the study with urine samples aeruginosa (14.5%), and least isolated was Eikenella, Morganella, in which the value of the PREVI Isola (bioM´erieux) system in the routine Showenella (0.8). diagnostic laboratory was analyzed (ECCMID 2009 − Ab.Nr. 1459). Conclusion: There are a number of possible pathogens but Staphylococ- PREVI Isola is a system for automated inoculation and streaking and cus aureus is by far the most common, that our study showed same result. is able to process any material from patients (liquid format). For urine Comparison of Antibiogram results that Treatment and evaluation of samples we observed less sub culturing and earlier identification resulting osteomyelitis infection according to E-test method was more successful in saving labor time and costs. The aim of this study was to explore the than kirby bauer method. We found that Eikenella corrodens can be usefulness of the PREVI Isola for more difficult samples as feces and cause of Chronic Osteomylitis. genital swabs. Methods: Feces or genital swabs from 100 different patients were R2128 Seroprevalence and risk factors of Helicobacter pylori processed manual and by the PREVI Isola. Fecal samples were cultured infection among schoolchildren in Algiers for Campylobacter (Campylobacter agar), Salmonella and Shigella (XLD-agar) and Yersinia (Yersinia agar). Genital swabs were cultured for S. Nait-Kaci °, N. Lamdjadani, Z. Guechi (Algiers, DZ) aerobic bacteria (blood agar), anaerobic bacteria (anaerobic blood agar), Neisseria gonorrhoeae (GO agar), Gardnerella vaginalis (Gardnerella Objective: The aim of our study is to evaluate the seroprevalence and agar) and for yeasts (Sabouraud agar). For the PREVI Isola both feces risk factors of Helicobacter pylori (Hp) infection in schoolchildren in (20 ml) and the genital swabs were suspended in 2.5 ml NaCl. Algiers. Results for fecal samples: All samples could be evaluated. No Material and Methods: We investigated 647 children (aged from 5 to Salmonella or Shigella was found. In 5 samples a Campylobacter was 18 years; mean age 11.5±3.3 years; sex ratio 0.85) from different public found but with the PREVI Isola individual, suspected colonies were schools, located in different districts of Algiers, chosen by drawing of better distinguished and were seen earlier (after 1 day). High counts lots. IgG anti Hp antibodies were detected by ELISA (Platelia Hp IgG of Yersinia were found in 1 sample but only with the PREVI Isola Biorad). Risk factors, collected from a questionnaire including: mother’s method. Results for genital swabs: In general counts of the different and father’s education levels, source water, presence or not of domestic bacteria were somewhat higher (+) with the PREVI Isola method than animals, and the localisation of the school. For statistical analysis we after manual inoculation. With PREVI Isola individual colonies of used Epi Info. the different bacteria were much better distinguished No difference in Results: Over all 70% of children were Hp seropositive. There was the isolation of gonococci (3 samples) was seen with both methods. not statically significant difference according to sex P = 0.06. The Gardnerella was 1 day earlier seen and much easier distinguished from seroprevalence according to age was 42.85% among children 5 and other bacteria with the PREVI Isola method. 6 years aged, 60.57% among 5−11 years; 77% among 12−15 years Conclusions: As with urine samples PREVI Isola leads to better readable and 85% among 16−18 years. There was not statically significant results for the more difficult cultures of feces and genital swabs: difference according to water source and presence of animals. The individual suspected colonies were better distinguished and counts were seroprevalence decreased when mother’s or father’s education level higher. Cultures were also often 1 day earlier positive for suspected increased (respectively P = 0.002 and P = 0.003). The seroprevalence rate colonies. Therefore PREVI Isola is very useful in the time consuming of Hp infection differed from a district to another one P = 0.04. Also we culture of especially genital swabs but also for fecal cultures in which find again a statically significant difference according to the promiscuity’s identification can be done earlier. rate P = 0.02. Conclusion: The seroprevalence rate in the school is high. Almost half of the children are infected at the age of schooling and rates increased R2127 Study of aetiologic agents in chronic osteomylitis with age. The infection is fond linked to poor socioeconomic conditions ° N. Veis Ahmadi, M. Izadi, A.A. Imani Fooladi , S.A. Mousavi (Zanjan, and to educational attainment of parents. Tehran, IR) Objectives: Osteomyelitis refers to an infection of the bone marrow Animal models including experimental which may spread to the bone cortex and periosteum via the haversian chanals. Osteomyelitis is an inflammation of bone caused by a payogenic treatment organism. Osteomyelitis has been categorized as acute, sub acute or R2129 Clinical study on therapeutic effect of mycocidine in chronic, with the presentation of each type based on the time of disease treatment of ringworm in cattle onset. (Occurrence of infection or injury). Investigation of prevalent factors which produce infection, also, Estimation and comparison of disk J. Akbarmehr ° (Sarab, IR) diffusion agar method and E-test method in order to determination of antibiotic sensibility about isolated bacteria from osteomyelitis infections Ringworm or dermatophytosis is an infectious and zoonotic disease are the main purpose of this study. caused by different species of ringworm fungi. Many species of animals Methods: 131patients with osteomyelitis infection disease were selected including humans are susceptible to ringworm infection. Cattle, horses, for this study during 2008–2009. Samples were collected from cats, dogs and domestic livestock are the most commonly affected bone (14.6%), tissue (7.3%), wound (66.1%), secretions (11.3%) and animals. Lesions of ringworm is usually found on the head, muzzle, S636 20th ECCMID, Abstracts accepted for publication only ears, neck, trunck and particulary around the eyes of infected animals. Methods: For this study 12 stainless steel chips and 12 HDPE chips These lesions are generally circular and oval in shape. Gray and dry to were used. E. coli strain was added to the beakers with TSB and the red and crusty hairless patches are typical of ringworm. samples. This study was conducted to determine the therapeutic effect of Results: Escherichia coli O111 formed biofilm with a mean cell density mycocidine(plant extracted antifungal drug)in treatment of ringworm in of 4.14±0.80, 7.69 ±0.19 log CFU/cm2 on stainless steel and HDPE cattle. 150 infected cattle with skin lesions of ringworm were treated respectively. There was significant difference (p < 0.05) between bacterial by mycocidine. Skin scales were collected by scraping of the lesion counts of two type surfaces. usig a strile scalpel in to apetri dish. Samples were used for direct Conclusion: Based on the results, it can be concluded that Escherichia microscopic examination and cultured on sabarouds dextrose agar for coli O111 can survive on milk contact surfaces e.g. stainless steel and isolation of ringworm fungi. All animals in this study were divided in HDPE surfaces forming biofilm. This is the first report, as far as we are two age groups (under 2years old and over 2 years old). Infected animals aware, of biofilm formation by Escherichia coli O111 on food contact were subjected for treatment with two times daily applications(locally stainless steel and HDPE surfaces. We were unable to find reports in our and topical)of mycocidine. Our resuls showed that rapid and effective search of the literature. cure in the most affected animals occurred 4−5 weeks after use of drug. Morever there was no significant difference in therapeutic rate R2132 Evaluation of different mechanisms of biofilm formation of two different age groups. Overall mycocidine was effective in both in clinical and commensal isolates of Staphylococcus age groups.%78cattle in over 2 years age and %81 those under 2years epidermidis age were cured completely after 5 weeks application of mycocidine. In conclusion the present study revealed that mycocidine is more effective M. Shahrooei °, J. Verhaegen, J. Van Eldere (Leuven, BE) in treatment of ringworm and its application should be recommended. Objectives: Currently, the two best-understood mechanisms of S. epi- dermidis biofilm formation are ica (intercellular adhesin)-dependent and Chlamydia and Burkholderia pulmonary diseases of the R2130 ica-independent proteinaceous biofilm formation. Ica-dependent biofilm Mycobacterium tuberculosis-susceptible I/St mice formation is characterized by ica-encoded polysaccharide synthesis; L. Nesterenko °, T. Stepanova, J. Alyapkina,