Evaluation of Biological Data, Guanella Pass Area, Clear Creek and Park Counties, Colorado, Water Years 1995-97 By Jennifer R. Cox-Lillis U.S. GEOLOGICAL SURVEY Open-File Report 00-54 Prepared in cooperation with the FEDERAL HIGHWAY ADMINISTRATION Denver, Colorado 2000 U.S. DEPARTMENT OF THE INTERIOR BRUCE BABBITT, Secretary U.S. GEOLOGICAL SURVEY Charles G. Groat, Director The use of firm, trade, and brand names in this report is for identification purposes only and does not constitute endorsement by the U.S. Geological Survey. For additional information write to: Copies of this report can be purchased from: District Chief U.S. Geological Survey U.S. Geological Survey Information Services Box25046, Mail Stop 415 Box 25286 Denver Federal Center Federal Center Denver, CO 80225-0046 Denver, CO 80225 CONTENTS Abstract.................................................................................................................................................................................. 1 Introduction................................................. 2 Purpose and Scope....................................................................................................................................................... 2 Description of Study Area........................................................................................................................................... 2 Acknowledgments....................................................................................................................................................... 5 Study Methods ...........................................................................................................................................^ 5 Study Design.....................................................................................................................^ 5 Sample Collection....................................................................................................^ 5 Water Quality..................................................................................................................................................... 5 Macroinvertebrates............................................................................................................................................ 6 Algae...........................................................................^ 7 Habitat.................................................................................................^ 7 Substrate Particle-Size Determination......................................................................................................................... 7 Data Analysis........................................................................................................................................................^ 7 Explanation of Macroinvertebrate Indexes........................................................................................................ 7 Explanation of Algal Computations .................................................................................................................. 8 Description of Environmental Factors................................................................................................................................... 9 Surface-Water Flow and Roadway Drainage............................................................................................................... 9 Water-Quality Constituents and Properties.................................................................................................................. 10 Nutrients ............................................................................................................................................................ 10 Properties.........................................................................................................._^ 13 Suspended Sediment.......................................................................................................................................... 14 Habitat Characteristics................................................................................................................................................. 14 Substrate Particle Size ................................................................................................................................................. 15 Results of Macroinvertebrate and Algal Data Analyses........................................................................................................ 16 Macroinvertebrates....................................................................................................................................................... 16 Algae............................................................................................................................................................................ 20 Summary and Conclusions .................................................................................................................................................... 36 References............................................................................................................._^ 37 Macroinvertebrate and Periphyton Data................................................................................................................................ 39 FIGURES 1. Map showing location of Guanella Pass study area and biological sample-collection sites..................................... 3 2. Graph showing annual mean discharge for Geneva Creek at Grant, Colorado, water years 1995-97...................... 10 3. Boxplots showing concentrations or values of selected water-quality constituents and properties for the Guanella Pass study area, water years 1995-97.................................................................................................. 11 4-19. Graphs showing: 4. Habitat characterization scores for biological sampling sites, Guanella Pass study area................................. 14 5. Total number of macroinvertebrate taxa in stream reaches, Guanella Pass study area, water years 1995-97......................................................................................................................................... 17 6. Macroinvertebrate density in stream reaches, Guanella Pass study area, water years 1995-97 ...................... 18 7. Shannon-Weaver diversity indexes for macroinvertebrates in stream reaches, Guanella Pass study area, water years 1995-97....................................................................................................................... 19 8. Relative abundance of macroinvertebrate functional feeding groups in stream reaches, Guanella Pass study area, wateryears 1995-97............................................................................................................... 21 9. Relative abundance of Ephemeroptera, Plecoptera, and Trichoptera (EPT) in stream reaches of the Guanella Pass study area, water years 1995-97......................................................................................... 22 10. Relative abundance of EPT:Chironomidae in stream reaches of the Guanella Pass study area, wateryears 1995-97......................................................................................................................................... 23 11. Algal density in stream reaches, Guanella Pass study area, wateryears 1995-97........................................... 25 CONTENTS III 12. Relative abundance of algal divisions, Guanella Pass study area, water years 1995-97 ................................. 26 13. Algal biovolume in stream reaches, Guanella Pass study area, water years 1995-97 ..................................... 27 14. Relative biovolume of algal divisions, Guanella Pass study area, water years 1995-97 ................................. 28 15. Shannon-Weaver diversity index for diatoms in stream reaches, Guanella Pass study area, water years 1995-97......................................................................................................................................... 30 16. Pollution Tolerance Index (PTI) for diatoms in stream reaches of the Guanella Pass study area, water years 1995-97................................................................................................................................ 31 17. Relative abundance of sensitive diatoms in stream reaches, Guanella Pass study area, water years 1995-97.............................................................................................................^ 32 18. Relative abundance of motile diatoms in stream reaches, Guanella Pass study area, water years 1995-97................................................................................................................................................... 33 19. Relative abundance of acid-tolerant diatoms in stream reaches, Guanella Pass study area, water years 1995-97......................................................................................................................................... 34 TABLES 1. Biological sampling sites in the Guanella Pass study area..................................................................................... 4 2. Years samples were collected at each site in the Guanella Pass study area, water years 1995-97........................ 4 3. Site descriptions for the biological sampling sites, Guanella Pass area, water years 1995-97 ............................. 6 4. Road-runoff linkages to biological