Official Journal of the American College of Sports Medicine Vol. 49 No. 5 Supplement S247 C-10 Thematic Poster ‑ Bioenergetics and Training RESULTS: Skeletal muscle CS maximal activity increased (P<0.05) from 25.5±3.1 to -1 -1 30.0±3.1 µmol·g ·min in HIT only, with larger (P<0.05) improvement compared to Thursday, June 1, 2017, 8:00 AM ‑ 10:00 AM MIT. Muscle HAD maximal activity increased (P<0.05) in HIT 15.3±1.9 to 18.5±4.0 -1 -1 -1 -1 Room: 403 µmol·g ·min and in MIT (15.7±2.8 to 19.5±3.0 µmol·g ·min ) with no between- group difference. YYIR2 score was improved (P<0.05) ~39 % more in HIT compared 1227 Chair: Gretchen A. Casazza. UC Davis Sports Medicine, to MIT post-intervention (323 ± 125 vs. 222 ± 113 m). Sacramento, CA. CONCLUSIONS: Additional high-intensity and moderate-intensity training augmented skeletal muscle oxidative capacity and high-intensity exercise performance (No relationships reported) in trained athletes with an overall higher effect of high-intensity training. 1228 Board #1 June 1 8:00 AM ‑ 10:00 AM 1230 Board #3 June 1 8:00 AM ‑ 10:00 AM Resistance Exercise Attenuates Mitochondrial Decreased Energy Availability During Intensified Function: Effects Of NSAID Intake And Eccentric- Training is Associated with Non-Functional Overload Training Overreaching in Female Runners Daniele Cardinale1, Mats Lilja2, Mirko Mandic2, Thomas Karine Schaal1, Marta Van Loan, FACSM1, Christophe Gustafsson2, Filip J. Larsen1, Tommy R. Lundberg2. 1The Hausswirth2, Gretchen Casazza1. 1UC Davis, Davis, CA. 2Institut Swedish School of Sport and Health Sciences, Stockholm, National du Sport, de l’Expertise et de la Performance (INSEP), Sweden (GIH), Stockholm, Sweden. 2Karolinska Institutet, Paris, France. (Sponsor: Marta Van Loan, FACSM) Stockholm, Sweden. Email: [email protected] Email: [email protected] (No relationships reported) (No relationships reported) PURPOSE: We examined the hypothesis that failing to maintain energy availability Although nonsteroidal anti-inflammatory drugs (NSAIDs) have been shown to (EA, calculated as energy intake (EI) - exercise energy expenditure (ExEE)) during modulate skeletal muscle adaptations and protein metabolism in response to resistance intensified training (IT) would predispose athletes to a state of overreaching (OR; high exercise, little is known about the effects of NSAIDs on mitochondrial function. Thus, perceived fatigue and prolonged performance impairment). the current study aimed to examine the effects of resistance exercise with concomitant METHODS: After 4 weeks of baseline training, 16 female runners (28 ± 5 yrs) NSAID consumption on mitochondrial oxidative phosphorylation in skeletal muscle. performed 4 weeks of IT (130% of baseline volume), followed by a 2 week recovery, Twenty participants were randomized in a single-blinded fashion to either an (REC, 50% of baseline). Over the last 7 days of each phase, ExEE was measured experimental group receiving ibuprofen (IBU: 27±5 yr; n=11; 1200 mg/d) or a control with chest-worn activity monitors (Actiheart) and EI was recorded using my Fitness group receiving a low-dose acetylsalicylic acid (CON: 26±4 yr; n=9; 75 mg/d) During Pal phone application. Running performance (distance covered during a graded this period, subjects performed 8 weeks of supervised resistance exercise involving the treadmill test) and perceived fatigue (REST-Q) were assessed at the end of each phase knee extensors muscles. Each of the subject’s legs were randomized to complete the to classify athletes as OR or acutely fatigued (AF, increased fatigue but no decrease training program using either a flywheel (FW) device emphasizing eccentric-overload, in performance). VO max, heart rate (HR), systolic blood pressure (SBP), plasma or a traditional weight stack machine (WS). Maximal mitochondrial oxidative 2 epinephrine and norepinephrine and blood lactate concentrations were measured at 65, phosphorylation (OXPHOS) from permeabilized skeletal muscle bundles was assessed 75 and 85% of VO max and immediately after maximal exercise. using high resolution respirometry before and after the training intervention. Citrate 2 RESULTS: 7 runners became OR and 9 were AF (Δperformance:-9±2% vs +4±2 %). synthase activity was assessed using spectrophotometric techniques. After training, Performance was still suppressed in OR after REC (-6 ± 5%). A significant decrease OXPHOS decreased (P<0.05) in both IBU (23%) and CON (29%) with no difference in EA was found in OR (-178±104 kcal/d), who failed to increase EI with IT. By across medical treatments. Although OXPHOS decreased in both legs, the decrease contrast, AF increased EI (184±48kcal/d) and maintained EA. ΔEA correlated with was greater (interaction P= 0.015) in WS (33%, P= 0.015) than in FW (19%, P= Δperformance and ΔVO max (R = 0.61 and 0.66, p < 0.05). VO max and peak lactate, 0.078). Citrate synthase (CS) did not change after the intervention. The increase 2 2 epinephrine and norepinephrine, HR and SBP were suppressed in OR, but were in quadriceps muscle volume was not significantly correlated with the change in maintained in AF after IT. At submaximal intensities at the same speed after IT, AF 2017 JUNE 1, THURSDAY, OXPHOS (R=0.15). These results suggest that 1) eight weeks of resistance training showed reduced HR, and lactate and norepinephrine responses, while OR showed no reduces mitochondrial function but not mitochondrial content, 2) The decreased change in these variables but did have increased ratings of perceived exertion. mitochondrial function with resistance exercise was not affected by ibuprofen CONCLUSIONS: Failure to maintain EA during IT was associated with a state consumption, 3) flywheel resistance training, emphasizing eccentric overload, rescues of non-functional OR in female runners. High perceived fatigue and impaired some of the reduction in mitochondrial function seen with conventional resistance performance in OR was accompanied by blunted physiological responses at maximal training. exercise and a lack of any desirable cardiovascular or endocrine-metabolic adaptation to submaximal intensities. In contrast, AF runners that increased EI to match ExEE 1229 Board #2 June 1 8:00 AM ‑ 10:00 AM with IT showed improved performance and lower HR, blood lactate and plasma Human Skeletal Muscle Oxidative Capacity Is Up- norepinephrine at the same submaximal speeds after IT. regulated After High-intensity Training In Competitive Soccer Players 1231 Board #4 June 1 8:00 AM ‑ 10:00 AM Dan Fransson1, Nikolai Baastrup Nordsborg2, Peter Krustrup3, The Effect of Aerobic and Resistance Training Exercise Magni Mohr4. 1University of Gothenburg, Gotheburg, Sweden. on Blood Pressure Response in College Aged 2University of Copenhagen, Copenhagen, Denmark. 3University Students 4 of southern Denmark, Odense, Denmark. University of the Mark Rudich, Randy Canivel, Bradley Warren. Southern Faroe Island, Torshavn, Faroe Islands. Methodist University, Dallas, TX. Email: [email protected] Email: [email protected] (No relationships reported) (No relationships reported) In sedentary persons, high-intensity training (HIT) induces a larger up-regulation of PURPOSE: College years are a time of learning and adopting positive healthy habits skeletal muscle oxidative capacity than moderate-intensity training (MOD). However, into one’s lifestyle, so it seems prudent to measure any changes in cardiovascular it is unknown if HIT compared to MOD also induces larger muscular oxidative profiles (i.e. blood pressure) Thus, the purpose of this study was to determine blood adaptations in trained athletes. PURPOSE: To investigate the hypothesis that HIT pressure (BP) responses in college-aged students enrolled in a 16-week Indoor induces larger changes in skeletal muscle oxidative capacity and high-intensity Cycling or Resistance-Training course. METHODS: Thirty-eight students (N =38) exercise performance than MOD in trained soccer players. volunteered for this study during The Fall 2015 Semester and signed a Human Subjects METHODS: In a randomized controlled trial, 31 competitive soccer players consent form. Inclusion criteria included the following: healthy males and females, (mean±SD, age, 22±2 years, height, 183±8 cm, weight, 76±6 kg) were assigned to normotensive, and non-smokers. Students were between the ages of 18-21 years either HIT (n=16) or a MOD (n=15). HIT performed 6-10 x 30 s all-out exercise bouts of age. Exclusion criteria included those who were symptomatic, on contradicting separated by 3 min recovery (high speed running distance of 238±51 m , peak blood medications, or habitual smokers. Students’ resting systolic and diastolic BP was lactate 13.7±3.4 mM), while MIT performed small-sided games (6v6 2 x 7-9 min with assessed pre-, mid, -and post- semester. RESULTS: Means and standard deviations 2 min recovery; high speed running distance of 14±14 m, peak blood lactate 4.8±2.3 (SD) were determined for age (21 ± 3.36 years), height (69 ± 4.07 in.), and weight (156 mM). The training-intervention was conducted three times per wk in 4 wks in addition ± 34.88 lbs.) A Paired Sample T-Test was utilized to compare blood pressure responses to the normal team-training. A muscle biopsy was obtained pre and post-intervention between classes. Statistical significance was seta priori at p≤ 0.05. Statistical analyses from m. vastus lateralis for analysis of 3-hydroxyacyl-CoA-dehydrogenase (HAD) revealed significant findings for mid-to-post- BP between aerobic (mid-121.24 ± and citrate synthase (CS) maximal enzyme activity. In addition, the Yo-Yo Intermittent Recovery test level 2 (YYIR2) was completed. Abstracts were prepared by the authors and printed as submitted. S248 Vol. 49 No. 5 Supplement MEDICINE & SCIENCE IN SPORTS & EXERCISE® 14.79; post-116.38 ± 12.76) and resistance training (mid- 124.53± 14.03; post- 122.76 knowledge, this is the first report of a significant improvement in ambulatory function ± 16.64) classes. CONCLUSIONS: Both classes experienced statistically, positive following chronic eccentric training. Improvement in ambulatory function would be changes in BP from mid- to post- semester.