BIOLOGY 327/388J GENERAL PHYCOLOGY FALL 2010 BIO 327 (#49505) & BIO 388J (#50465): 11:00 a.m. MWF BIO 301 BIO 127L (#49510) & BIO 188K (#50470): 1-4:00 p.m. W BIO 12H Instructor: Dr. J. W. La Claire 325 Biol. Lab. (471-3577) Office Hours: M & F 1:00-2:30 p.m. or by appointment E-mail address: [email protected] Assistant: TBA Textbook: "Algae”, 2nd Edition, L.E. Graham et al. 2009, Pearson Benjamin Cummings, San Francisco. Course Webpage: http://www.bio.utexas.edu/faculty/laclaire/bio327lac/default.htm The username and password for this site will be distributed in class. Course Objectives: to survey the various organisms collectively classified as algae, on a comparative basis. Structure, reproduction and evolution will be stressed. Because many algae have economic significance, there will also be an emphasis on the uses of algae. This will emphasize the use of algae as dynamic, model organisms for experimental biology - a topic that will be experienced firsthand in laboratory. Grading and Organization: there will be two “midterm” examinations and a final examination. Although exams will be designed to be completed in much less time, a three-hour period is reserved for each, so that time constraints will not be a factor. Each exam will be worth 100 points. There are no make-up examinations. An additional 50 points will be assessed in lab, based upon the quantity and quality of participation, and on specific in-lab exercises. The final grade will be determined on the basis of the total 350 points. Practice (announced) quizzes may be given in lab, to prepare for the exams, but the scores will not figure into the final grade. Please note that lecture and laboratory are co- requisites. A student must be enrolled in both to obtain credit for either. Because the laboratories and lectures are so interdependent, a single final grade will be assessed and it will be assigned as both the lecture and lab grade. In other words, the grades for lecture (BIO 327 or 388J) and lab 1 (BIO 127L or 188K) will be identical. If you drop either lecture or lab, the other must also be dropped. Most laboratory exercises have an experimental component in addition to an observational portion. The lab exercises will be posted on the course website in advance of each lab, so you should download and print out the appropriate exercise as that lab approaches. For efficient operation and to obtain maximal laboratory points, it is essential for each student to: 1) read the exercise before coming to lab, 2) be in lab on time in order to receive last- minute instructions and cautions, and 3) begin the work in a timely fashion. For everyone's safety, any student not complying with these or other stated laboratory rules (or otherwise posing a hazard to the class) may be dismissed from the lab that day, losing the appropriate number of points from their total score. Frequently, two or more students will collaborate in small teams. However, each student should perform every portion of the exercise, unless instructions state otherwise. It is strongly recommended that each person make simple drawings of the algae seen in the observational periods of the labs, since this technique greatly helps one to remember them. Official drop dates are: Sept. 10 (12th Class Day) is the last day to drop with possible refund. Sept. 22 ("Q" Drop) is the last day to drop without possible academic penalty. Oct. 20 is the last possible day to drop or withdraw with the Dean's approval. Policy Statement on Scholastic Dishonesty: students who violate University rules on scholastic dishonesty are subject to disciplinary penalties, including the possibility of failure in the course and/or dismissal from the University. Since such dishonesty harms the individual, all students, and the integrity of the University and its degrees, policies on scholastic dishonesty will be strictly enforced. Students with disabilities: The University of Texas at Austin provides upon request appropriate academic accommodations for qualified students with disabilities. For more information, contact the Office of the Dean of Students at 471-6259, 471- 6441 TTY. 2 APPROXIMATE LECTURE SCHEDULE (includes relevant readings in “Algae”, 2nd Edition) Aug. 25 Introduction - Definition & General Classification 27 Biochemistry of Algal Cells (1-5,8-17) Aug. 30 Structure of Algal Cells (62-77) Sept. 1 Life History Concept & Reproduction (5-8) 3 Cyanobacteria (bluegreen algae) (94-108) 6 No Class - Labor Day Holiday 8 Cyanobacteria (108-121) 10 Endosymbiosis and Algal Evolution (78-93,122-139) 13 Green Algae - General Features (353-365) 15 Green Algae - Prasinophyceae (365-372) 17 Green Algae - Chlorophyceae (412-442) 20 Green Algae - Chlorophyceae (443-444) 22 Green Algae - Ulvophyceae (Codiolales & Ulvales) (373-384) [Lab Period: MIDTERM #1 - through Prasinophyceae] 24 Green Algae - Ulvophyceae (Caulerpales) (393-400) 27 Green Algae - Ulvophyceae (Dasycladales) (401-403) 29 Green Algae - Ulvophyceae (Cladophorales) (387-393) Oct. 1 Green Algae - Ulvophyceae (Trentepohliales) & Trebouxiophyceae(384-387, 404-411) 4 Green Algae - Charophyceae (Klebsormidiales & Coleochaetales) (443-452, 470-476) 6 Green Algae - Charophyceae (Zygnematales) (453-470) 8 Green Algae - Charophyceae (Zygnematales) 11 Green Algae - Charophyceae (Charales) (476-485) 13 Green Algae - Charophyceae & Evolution of the Green Algae 15 Chlorarachniophytes (139-144) 18 Euglenoid Flagellates (146-158) 20 Dinoflagellates (144-145, 186-201) 22 Dinoflagellates (201-216) 25 Cryptophytes (159-169) 27 Haptophytes & Heterokont Algae (Stramenopiles) (170-185, 217-222) [Lab Period: MIDTERM #2 - Chlorophyceae through Euglenoids] 29 Heterokont Algae – Eustigmatophytes, Raphidophytes & Xanthophytes (261-267, 273-278) 3 Nov. 1 Heterokont Algae - Chrysophytes (247-261, 267-271) 3 Heterokont Algae - Diatoms (223-234) 5 Heterokont Algae - Diatoms (234-246) 8 Heterokont Algae - Brown algae (278-288) 10 Heterokont Algae - Brown algae (293-295) 12 Heterokont Algae - Brown algae (288-291) 15 Heterokont Algae - Brown algae (295-304) 17 Heterokont Algae - Brown algae (304-308) 19 Heterokont Algae - Brown algae 22 Red Algae (309-324) 24 Red Algae (324-335) 26 No Class - Thanksgiving Holiday 29 Red Algae (335-344) Dec. 1 Red Algae (344-352) 3 Red Algae, Summary Comments FINAL EXAMINATION: Wednesday, December 8, 9:00 a.m. – 12:00 noon (location to be determined) 4 PHYCOLOGY LABORATORY SCHEDULE FALL 2010 WEEK Group Survey & Experimental Exercise 1. No Laboratory 2. Diversity of Algae; Comparisons Between Lower and Higher Plant Morphology & Use & care of the light microscope 3. Cyanophyta & Pigment (water soluble) Extraction from Freymella & Chromatic Adaptation 4. Glaucophytes & Plasmid Isolation from Phormidium 5. Midterm Examination #1 6. Chlorophyta I (Prasinophyceae, Chlorophyceae & Ulvophyceae) & Phototaxis in Chlamydomonas 7. Chlorophyta II (Bryopsidophyceae, Dasycladophyceae & Cladophorophyceae) & Wound healing in Siphonocladales & Turgor Pressure Determination in Valonia 8 Chlorophyta III (Klebsormidiophyceae, Zygnematophyceae) & Flagellar Regeneration in Chlamydomonas (& follow-up on wound healing responses from last time) 9. Chlorophyta IV (Charophyceae), Chlorarachniophyta, Euglenophyta, Pyrrophyta & Cytoplasmic Streaming in Nitella. Demonstration of Bioluminescence 10. Midterm Examination #2 11. Cryptophyta, Haptophyta, Chrysophyceae, Xanthophyceae, Eustigmatophyceae, Raphidophyceae (no experimental portion today) 12. Bacillariophyceae & diatom identification & Cell Locomotion (gliding motility) in Pennate Diatoms 13. Phaeophyta I & Pigment (lipid soluble) Extraction from Chlamydomonas 14. Phaeophyta II & Rhodophyta I & Cell Fusion in Griffithsia 15. Rhodophyta II (follow-up on cell fusion experiments from last time) 5 .