ENVIRONMENTAL INSTITUTE, s.r.o., Okružná 784/42, 972 41 Koš Annex I Analysis of metals in surface water samples Environmental Institute, s.r.o., Okružná 784/42, 972 41 Koš, Slovakia October 2020 EUWI+, Investigative monitoring of the Dnieper River Basin, October 2020 ENVIRONMENTAL INSTITUTE, s.r.o., Okružná 784/42, 972 41 Koš Surface water samples Cd (ug/L) Pb (ug/L) Hg (ug/L) Ni (ug/L) As (ug/L) Cr (ug/L) Cu (ug/L) Zn (ug/L) Metals – limit values 0.15** 1.2** 0.07*** 4** 24* 9.0* 1.6* 9.6* Table 1: Results of analyses of Sampling point 1 filtered <LOQ <LOQ <LOQ <LOQ 3.38 <LOQ 1.15 4.0 metals in surface water samples. Sampling point 2 filtered 0.547 <LOQ <LOQ 1.845 <LOQ 0.666 1.94 26.5 The values represent dissolved Sampling point 3 filtered <LOQ <LOQ <LOQ <LOQ 1.99 <LOQ 0.89 4.1 concentrations (after filtration of Sampling point 4 filtered <LOQ <LOQ <LOQ 1.33 <LOQ <LOQ 4.21 9.2 the samples). Numbers Sampling point 5 filtered 2.66 <LOQ <LOQ <LOQ <LOQ 2.35 9.39 20.6 highlighted in orange colour Sampling point 6 filtered <LOQ <LOQ <LOQ <LOQ 2.62 <LOQ 4.95 6.9 indicate exceedance of the Sampling point 7 filtered 0.345 <LOQ <LOQ <LOQ <LOQ 0.777 6.06 38.4 toxicity threshold values. Sampling point 8 filtered <LOQ <LOQ <LOQ <LOQ 2.65 <LOQ 1.03 61.7 Sampling point 9 filtered 0.129 <LOQ <LOQ <LOQ 2.29 0.85 5.36 16.4 Sampling point 10 filtered <LOQ <LOQ <LOQ 1.772 <LOQ <LOQ <LOQ 7.9 Sampling point 11 filtered 0.292 <LOQ <LOQ <LOQ <LOQ 1.31 0.82 11.9 Sampling point 12 filtered 0.328 <LOQ <LOQ <LOQ <LOQ 3.89 1.34 2.6 Sampling point 13 filtered <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.81 10.0 Sampling point 14 filtered <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 1.25 <LOQ Sampling point 15 filtered <LOQ <LOQ <LOQ <LOQ 3.30 <LOQ 2.58 20.0 Sampling point 16 filtered 0.408 <LOQ 0.04 2.515 3.72 0.186 2.86 8.8 Sampling point 17 filtered 0.092 <LOQ <LOQ 1.066 <LOQ <LOQ 1.38 18.8 Sampling point 18 filtered 0.097 <LOQ <LOQ <LOQ <LOQ <LOQ 0.82 <LOQ Sampling point 19 filtered 0.106 <LOQ <LOQ 1.982 2.27 <LOQ 1.62 17.2 Sampling point 20 filtered 0.155 <LOQ <LOQ 1.378 <LOQ 0.472 1.00 0.7 Sampling point 21 filtered <LOQ <LOQ <LOQ 1.11 4.91 <LOQ 1.97 7.8 Sampling point 22 filtered <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 1.29 30.3 Sampling point 23 filtered <LOQ <LOQ <LOQ <LOQ 4.95 <LOQ <LOQ 17.1 Sampling point 24 filtered <LOQ <LOQ <LOQ 1.641 <LOQ <LOQ 1.52 23.5 Sampling point 25 filtered <LOQ <LOQ <LOQ 1.339 3.01 <LOQ 2.58 20.9 Sampling point 26 filtered <LOQ <LOQ <LOQ 6.142 <LOQ <LOQ 1.25 16.5 Sampling point 27 filtered 0.105 <LOQ <LOQ 2.784 10.6 <LOQ 0.90 35.9 LOQ (ug/L) 0.091 0.780 0.035 0.977 1.742 0.180 0.550 0.108 * - AA-EQS in the Slovak legislation 2013 ** - AA-EQS for inland surface waters in the Directive 2013/39/EU *** - MAC-EQS for inland surface waters in the Directive 2013/39/EU EUWI+, Investigative monitoring of the Dnieper River Basin, October 2020 ENVIRONMENTAL INSTITUTE, s.r.o., Okružná 784/42, 972 41 Koš Annex II List of compounds included in the wide-scope target screening Environmental Institute, s.r.o., Okružná 784/42, 972 41 Koš, Slovakia October 2020 EUWI+, Investigative monitoring of the Dnieper River Basin, October 2020 ENVIRONMENTAL INSTITUTE, s.r.o., Okružná 784/42, 972 41 Koš Summary table of 2252 target compounds analysed in surface water and biota samples within the wide-scope target screening LOD LOQ NORMAN ID Analyte (ng/L) (ng/L) NS00006968 Aceclidine 1.51 4.99 NS00000453 Acetamiprid 4.90 16.2 NS00009677 Albendazole sulfone 1.17 3.86 NS00007702 Amantadine 2.26 7.45 NS00010870 Aminobenzimidazole (2-) 4.46 14.7 NS00000633 Amisulpride-N-Oxide 2.33 7.68 NS00000412 Amphetamine-Methyl (methamphetamine) 24.6 81.2 NS00000226 Antipyrine- 4-Acetamido 1.74 5.76 NS00000329 Antipyrine- 4-Formylamino 1.03 3.39 NS00000470 Atazanavir 2.24 7.41 NS00010385 Atenolol 7.38 24.4 NS00000314 Atenolol acid (Metoprolol acid) 6.37 21.0 NS00000262 Atrazine 2.59 8.55 NS00000255 Atrazine-2-hydroxy 4.74 15.6 NS00000254 Atrazine-desethyl-2-hydroxy (=Prometon-Hydroxy-Desisopropyl) 8.49 28.0 NS00009778 Azoxystrobin 0.783 2.58 NS00000374 Azoxystrobin acid 6.99 23.1 NS00000060 Benzamidine 1.18 3.88 NS00010260 Benzothiazole- 2-Amino 2.96 9.78 NS00008199 Benzothiazole -2-OH 1.73 5.72 NS00010261 Benzotriazole (BTR) 2.74 9.03 NS00008943 Benzotriazole- 5-Me / Benzotriazole -4-Me 3.57 11.8 NS00009929 Bisoprolol 5.41 17.9 NS00000437 Boscalid 1.93 6.36 NS00000273 Caffeine 2.73 9.02 NS00009281 Candesartan 36.7 121 NS00000207 Carbamazepine 1.03 3.41 NS00010437 Carbamazepine -10-Hydroxy 1.03 3.41 NS00008351 Carbamazepine-10.11-dihydro-10.11 dihydroxy 13.1 43.3 NS00000327 Carbamazepine-10.11-epoxide 13.9 45.8 NS00010265 Carbendazim 3.46 11.4 NS00000627 Cetirizine-N-Oxide 38.7 128 NS00008150 Climbazole 0.593 1.96 NS00010041 Clomazone (Command) 1.06 3.49 NS00008954 Clopidogrel 7.01 23.1 NS00000417 Clopidogrel Carbon acid 6.18 20.4 NS00009180 Clothiandin 2.87 9.46 NS00010263 Cotinine 1.80 5.93 NS00010478 Cotinine-Hydroxy 3.66 12.1 NS00000339 Cyclophosphamide 2.23 7.35 NS00000117 Cyproconazole 5.96 19.7 NS00000118 Cyprodinil 0.278 0.918 EUWI+, Investigative monitoring of the Dnieper River Basin, October 2020 ENVIRONMENTAL INSTITUTE, s.r.o., Okružná 784/42, 972 41 Koš NS00000221 DEET (Diethyltoluamide) 0.233 0.769 NS00000212 Diclofenac 2.07 6.82 NS00010630 Didecyldimethylammonium (DADMAC (C10:C10)) 0.283 0.935 NS00000433 Difenoconazole 3.72 12.3 NS00000126 Dimetridazole 5.88 19.4 NS00008293 Dimoxystrobin 1.47 4.86 NS00010439 Diphenhydramine 1.42 4.70 NS00000265 Diuron 0.974 3.22 NS00000408 EDDP 2.87 9.48 NS00000604 Efavirenz 12.2 40.3 NS00009136 Epoxiconazole 0.932 3.08 NS00010438 Ethofumesate 8.97 29.6 NS00000281 Fluconazole 11.5 38.0 NS00008659 Fluoxastrobin 0.387 1.28 NS00000704 Flutriafol 1.21 3.98 NS00000303 Galaxolidone 6.81 22.5 NS00002652 Gibberellic acid 2.11 6.95 NS00011725 Griseofulvin 0.264 0.871 NS00000361 Imidacloprid 0.709 2.34 NS00000387 Irbesartan 7.47 24.7 NS00000260 Isoproturon 8.85 29.2 NS00000671 Isoxaben 0.731 2.41 NS00000216 Ketoprofen 4.06 13.4 NS00000354 Lamotrigine 7.94 26.2 NS00000419 Levamisol 2.55 8.43 NS00000027 Lidocaine 26.2 86.4 NS00011458 Lidocaine-Nor 2.61 8.61 NS00000595 Lidocaine-N-oxide 3.60 11.9 NS00008464 Linezolid 12.9 42.6 NS00008581 Lopinavir 0.878 2.90 NS00002063 Loratadine 0.262 0.864 NS00008822 Losartan 5.04 16.6 NS00000218 Mefenamic acid 17.6 58.1 NS00000622 Memantine 1.28 4.22 NS00003738 Metalaxyl 0.769 2.54 NS00010410 Metamitron 1.82 5.99 NS00010520 Metamitron-desamino 0.912 3.01 NS00000249 Metazachlor 0.891 2.94 NS00000558 Metconazole 1.83 6.04 NS00000333 Metformin 1.34 4.41 NS00000411 Methadone 3.89 12.9 NS00010464 Metobromuron 1.11 3.65 NS00000248 Metolachlor 0.0613 0.202 NS00000295 Metolachlor-ESA 5.32 17.6 NS00000059 Metolachlor-morpholinon 2.16 7.14 NS00000197 Metoprolol 3.32 11.0 EUWI+, Investigative monitoring of the Dnieper River Basin, October 2020 ENVIRONMENTAL INSTITUTE, s.r.o., Okružná 784/42, 972 41 Koš NS00000455 Metribuzin-Desamino (DA) 3.00 9.90 NS00000213 Naproxen 21.3 70.4 NS00008411 Nicosulfuron 1.20 3.97 NS00010308 Octocrylene 51.4 170 NS00010016 Oxcarbazepine 0.672 2.22 NS00000287 Phenazone 2.94 9.70 NS00000585 Pheniramine N-Oxide 4.23 13.9 NS00009400 Phosphate-triethyl 1.11 3.67 NS00010268 Phthalate-Dimethyl 4.68 15.4 NS00010095 Phthalate-Di-n-butyl 13.2 43.6 NS00010885 Prochloraz 1.81 5.98 NS00010269 Prometryn 0.328 1.08 NS00000456 Propazine-2-hydroxy (Prometon-Hydroxy) 6.22 20.5 NS00000220 Propiconazole 3.09 10.2 NS00010600 Propoxur 4.58 15.1 NS00010886 Prothioconazole-desethio 3.75 12.36 NS00000390 Pyraclostrobin 0.265 0.876 NS00000340 Ritonavir 16.2 53.6 NS00000195 Sotalol 3.22 10.6 NS00000199 Sulfadimidine (Sulfamethazine) 12.9 42.7 NS00000233 Sulfamethazine-N4-Acetyl 6.82 22.5 NS00000268 Sulfamethoxazole 9.82 32.4 NS00000269 Sulfamethoxazole-N4-Acetyl (Impurity A) 20.9 68.8 NS00000225 Sulfapyridine 6.83 22.6 NS00000201 Sulfathiazole 19.6 64.8 NS00000280 Tebuconazole 0.401 1.32 NS00000258 Terbuthylazine 8.31 27.4 NS00007860 Terbuthylazine-desethyl 21.5 71.0 NS00007958 Thiabendazole 1.00 3.31 NS00000448 Thiacloprid 1.25 4.11 NS00000449 Thiacloprid-amide 19.7 65.1 NS00010175 Thiamethoxam 1.84 6.09 NS00000551 Tributylamine 0.281 0.929 NS00000211 Trimethoprim 4.51 14.9 NS00000341 Valsartan 5.70 18.8 NS00000347 Venlafaxine-D L-N O-Didesmethyl 4.02 13.2 NS00000630 Venlafaxine-N-oxide 2.76 9.10 NS00010634 2-Benzothiazolesulfonic acid 87.6 289 NS00011447 3,3-pentamethylene-4-butyrolactam 1.02 3.35 NS00010632 Benzophenone-4 69.7 230 NS00010610 Bisphenol S 30.0 99.1 NS00010591 Lauryl diethanolamide 0.851 2.81 NS00007448 Metazachlor BH 479-11 1.37 4.52 NS00014432 N-Cyclohexyl-2-benzothiazol-amine 0.337 1.11 NS00010386 Phenylbenzimidazole sulfonic acid 45.2 149 NS00001149 Picoxystrobin 1.53 5.06 EUWI+, Investigative monitoring of the Dnieper River Basin, October 2020 ENVIRONMENTAL INSTITUTE, s.r.o., Okružná 784/42, 972 41 Koš NS00008140 Triethylcitrate 1.88 6.19 NS00000310 Secbumeton 0.639 2.11 NS00000584 Guanylurea 70.7 233 NS00000247 2.4-D 30.6 101 NS00009697 5-Chlorobenzotriazole 0.927 3.06 NS00000236 Bentazone 1.15 3.78 NS00000500 Chloramphenicol 0.155 0.512 NS00010496 Dinitrophenol-2-4- (DNP) 0.0292 0.0965 NS00014401 Diphenylphosphate 9.87 32.6 NS00000349 Fipronil 0.816 2.69 NS00000351 Fipronil-sulfide 1.17 3.86 NS00011514 Fludioxonil 0.305 1.01 NS00008630 Furosemide 27.1 89.3 NS00000343 Hydrochlorothiazide 29.5 97.3 NS00000159 Isofenphos 5.29 17.5 NS00000243 MCPA 14.8 49.0 NS00000697 Meclofenamic Acid 7.77 25.6 NS00001849 Methylparaben 0.434 1.43 NS00010148 Metolachlor CGA 368208 6.67 22.0 NS00007746 N-Butylbenzenesulfonamide