Wiseman CV R. Luke Wiseman, Ph.D. Associate Professor Department of Molecular Medicine The Scripps Research Institute MEM 220 La Jolla, CA 92037 Phone: (858) 784-8820 Email: [email protected] Education/Training 9/2005 – 8/2009 Skirball Institute at NYU Medical School New York, NY NIH Postdoctoral Research Fellow 7/2001 – 8/2005 The Scripps Research Institute La Jolla, CA Ph.D. Chemistry 9/1997 – 5/2001 The University of Virginia Charlottesville, VA B.Sc. Chemistry Positions/Employment 12/2016 – present The Scripps Research Institute La Jolla, CA Associate Professor (tenured) 8/2009 – 12/2016 The Scripps Research Institute La Jolla, CA Assistant Professor 9/2005 – 8/2009 Skirball Institute at NYU Medical School New York, NY NIH Postdoctoral Research Fellow; Advisor – David Ron 7/2001 – 8/2005 The Scripps Research Institute La Jolla, CA Graduate Research Associate; Advisor – Jeffery W. Kelly 8/2000 – 5/2001 University of Virginia Charlottesville, VA Undergraduate Research Associate; Advisor – Milton Brown 9/1999 – 7/2000 University of Virginia Charlottesville, VA Undergraduate Research Associate; Advisor – Felicia Etzkorn 5/1999 – 8/1999 Cornell University (REU Program) Ithaca, NY Undergraduate Research Associate; Advisor – David Zax Teaching Experience Wiseman CV 2017-present The Scripps Research Institute La Jolla, CA Lecturer – Chemical Biology I 2014-present The Scripps Research Institute La Jolla, CA Course Organizer – Cell Biology 2012-present The Scripps Research Institute La Jolla, CA Lecturer – Structural Biology 2011 – present The Scripps Research Institute La Jolla, CA (every 2 yrs) Course Organizer – Protein Folding in the Cell 2010-2016 The Scripps Research Institute La Jolla, CA (every 3 yrs) Course Co-Organizer – Chemical Biology I 2012-2013 The Scripps Research Institute La Jolla, CA Lecturer – Cell Biology 2004 The Scripps Research Institute La Jolla, CA Graduate Teaching Assistant – Bioorganic Chemistry I Other Committees Served 2019-present Member TSRI Genomics Core Faculty Advisory Committee 2018-present Member of the TSRI Presidential Planning Committee 2018-present Member Outreach, Inclusion, Committee Engagement Committee 2018-present Member Research Training Committee 2017-present Co-organizer of the Mesa-Wide ER stress club 2016-present Member TSRI Graduate Student Admissions Committee 2016-present Member Molecular Medicine Departmental Seminar Series Committee 2009-present Chair or member of >20 TSRI graduate student thesis committees 2012-2016 Co-organizer of TSRI Junior Faculty Luncheon Awards/Honors Glenn Award for Research in Biological Mechanisms of Aging, 2017 Amyloidosis Foundation Jr. Research Grant Award, 2014 Ellison Medical Foundation New Scholar Award in Aging, 2011 NRSA Kirschstein Postdoctoral Research Fellowship, 2006 Norton B. Gilula Graduate Fellowship, The Scripps Research Institute, 2004 Fletcher Jones Foundation Graduate Fellowship, The Scripps Research Institute, 2003 Oscar R. Rodig Award for Chemical Excellence, University of Virginia, 2001 Graduated with Highest Distinction, University of Virginia, 2001 Dean’s List, University of Virginia, 1998-2001 Wiseman CV Intermediate Honors, University of Virginia, 2000 Research Experience for Undergraduates, National Science Foundation, 1999 Peer-Reviewed Publications (2013-present) Puchades, C; Ding, B; Song, A; Wiseman, RL; Lander, GC; Glynn, SE (2019) Unique Structural Features of the Mitochondrial AAA+ Protease AFG3L2 Reveals the Molecular Basis for Activity in Health and Disease (2019) Mol Cell, in press (PMID: 31327635). Plate, L; Rius, B; Nguyen, B; Genereux, JC; Kelly, JW; Wiseman, RL (2019) Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized, Amyloidogenic Protein, Cell Chem Biol, in press (PMID: 31105062) Carreras-Sureda, A; Jana, F; Ramos-Fernandex, E; Urra, H; van Vliet, AR; Durand, S; Mortensen, DE; Pihan, P; Gonzalez-Quirez, M; Saredo, A; Bustos, G; Vincente, R; Inestrosa, NC; Wiseman, RL; Agonstinis, A; Bultynck, G; Court, F; Kroemer, G; Cesar- Cardenas, J; Hetz, C (2019) Non-Canonical Function of the Unfolded Protein Response Sensor IRE1α as a Structural Determinant of Mitochondrial-Associated ER Membranes, Nat Cell Biol, in press (PMID: 31110288) Grandjean, JMD; Plate, L; Morimoto, RI; Bollong, MJ; Powers, ET; Wiseman, RL (2019) Deconvoluting Stress-Responsive Signaling Pathway for Pharmacologic Activation Using Targeting RNA-Sequencing, ACS Chemical Biology, in press (PMID: 30821954). Blackwood, EA; Azizi, K; Thuerauf, DJ; Paxman, RJ; Plate, L; Kelly, JW; Wiseman, RL; Glembotski, CC (2018) Pharmacologic ATF6 Activation Confers Global Protection in Widespread Disease Models by Reprogramming Cellular Proteostasis, Nature Communications, 10, 187-195 (PMID: 30643122). Romine, IC; Wiseman, RL (2018) PERK Regulates Extracellular Proteostasis of an Amyloidogenic Protein, Sci Rep, 9, 410-414 (PMID: 30675021). Paxman, RJ; Plate, L; Blackwood; EA; Glembotski, CC; Powers, ET; Wiseman, RL*; Kelly, JW* (2018) A Small Molecule that Preferentially Activates the ATF6 Pathway of the Unfolded Protein Response is Metabolically Activated to Selectively Modify Endoplasmic Reticulum Proteins, ELIFE, in press (PMID: 30084354). Giadone, RM; Rosarda, JD; Reddy Akepati, P; Thomas, AC; Boldvbataar, B; James, MF; Wilson, AA; Sanchorawala, V; Connors, LH; Berk, JL; Wiseman, RL; Murphy, GJ (2018) A genetically diverse library of ATTR patient-specific induced pluripotent stem cells for disease modeling and in vitro testing of novel therapeutics, Amyloid, in press (PMID: 30032658). Wiseman CV Cole, KS; Grandjean, JMD; Chen, K; Witt, CH; O’Day, J; Shoulders, MD; Wiseman, RL; Weerapana, E (2018) Characterization of an A-Site Selective Protein Disulfide Isomerase A1 Inhibitor, Biochemistry, 57, 2035-2043 (PMID: 29521097). Lebeau, J; Saunders, JM; Moraes, VWR; Madhavan, A; Madrazo, N; Anthony, MC; Wiseman RL (2018) The PERK Arm of the Unfolded Protein Response Regulates Mitochondrial Morphology During Acute Endoplasmic Reticulum Stress, Cell Reports, 22, 2827-2836 (PMID: 295394013). Kroeger, H; Grimsey, N; Paxman, R; Plate, L; Jones, Y; Shaw, PX; Trejo, J; Tsang, SH; Powers, ET; Kelly, JW; Wiseman, RL; Lin, JH (2018) The Unfolded Protein Response Regulator ATF6 Promotes Mesodermal Differentiation, Science Signaling, in press (PMID: 29440509). Puchades, C; Rampello, AJ; Giuliano, CJ; Shin, M; Wiseman, RL; Glynn, SE; Lander GC (2017) Structure of the Mitochondrial Inner Membrane AAA+ Protease YME1 Reveals the Mechanism of Substrate Processing, Science, in press (PMID: 29097521). Chen, KC; Qu, S; Chowdhury, S; Noxon, IC; Schonhoft, JD; Plate, L; Powers, ET; Kelly, JW; Lander, GC; Wiseman, RL (2017) The Endoplasmic Reticulum HSP40 Co- chaperone ERdj3/DNAJB11 Assembles and Functions as a Tetramer, EMBO J, 36, 2296-2309 (PMID: 28655754). Chen, JJ; Genereux, JC; Suh, EH; Vartabedian, VF; Rius, B; Qu, S; Dendle, MTA; Kelly, JW; Wiseman, RL (2016) Endoplasmic Reticulum Proteostasis Influences the Oligomeric State of an Amyloidogenic Protein Secreted from Mammalian Cells, Cell Chemical Biology, 23, 1282-1293 (PMID: 27720586). Plate, L; Cooley, CB; Chen, JJ; Paxman, RJ; Gallagher, CM; Madoux, F; Genereux, JC; Dobbs, W; Garza, D; Spicer, TP; Scampavia, L; Brown, SJ; Rosen, H; Powers, ET; Walter, P; Hodder, P; Wiseman, RL*; Kelly JW* (2016) Small Molecule Proteostasis Regulators that Reprogram the ER to Reduce Extracellular Protein Aggregation, ELIFE, in press (PMID: 27435961). *Co-corresponding authors. Rainbolt, TK; Lebeau, J; Puchades, C; Wiseman, RL (2016) Reciprocal Degradation of YME1L and OMA1 Adapts Mitochondrial Proteolytic Activity During Stress, Cell Reports, 14, 2041-2049 (PMID: 26923599). Murray, AN; Chen, W; Antonopoulos, A; Hanson, SR; Wiseman, RL; Dell, A; Haslam, SM; Powers, DL; Powers, ET; Kelly, JW (2015) Enhanced Aromatic Sequons Increase Oligosaccharyltransferase Glycosylation Efficiency and Glycan Homogeneity, Chemistry & Biology, 22, 1052-1062 (PMID: 26190824). Baranczak, A; Liu, Yu; Connelly, Stephen; Han Du, W; Greiner, ER; Wiseman, RL; Eisele, YS; Bradbury, NC; Dong, J; Sharpless, KB; Noodleman, L; Wilson, IA; Encalada, Wiseman CV SE; Kelly, JW (2015) A Fluorogenic Arylfluorosulfate for Intraorganellar Transthyretin Imaging in Living Cells and in C. elegans, J. Am. Chem. Soc., 137, 7404-7414 (PMID: 26051248). Rainbolt, TK; Saunders, JM; Wiseman RL (2015) YME1L Degradation Reduces Mitochondrial Proteolytic Capacity During Oxidative Stress, EMBO Reports, 16, 97-106 (PMID: 25433032). Genereux, JC; Qu, S; Zhou, M; Ryno, LM; Wang, S; Shoulders MD; Kaufman, RJ; Lasmezas, CI; Kelly, JW; Wiseman RL (2015) Unfolded Protein Response-Induced ERdj3 Secretion Links ER Stress to Extracellular Proteostasis, EMBO J, 34, 4-19 (PMID: 25361606). F1000 recommended. Chen, JJ; Genereux, JC; Qu, S; Hulleman, JD; Shoulders, MD; Wiseman, RL (2014) Increasing ER Quality Control Stringency Through ATF6 Activation Reduces the Secretion and Extracellular Aggregation of Destabilized Variants of an Amyloidogenic Proteins, Chemistry & Biology, 21, 1564-1574 (PMID: 25444553). Cooley, CB; Ryno, LM; Plate, L; Morgan, GJ; Hulleman, JD; Kelly, JW; Wiseman, RL (2014) Unfolded Protein Response Activation Reduces Secretion and Extracellular Aggregation of Amyloidogenic Immunoglobulin Light Chain, Proc. Natl. Acad. Sci. USA, 111, 13046-13051 (PMID: 25157167). Ryno, LM; Genereux, JC; Naito, T; Morimoto, RI; Powers, ET; Shoulders, MD; Wiseman RL (2014) Characterizing the Altered Cellular Proteome Induced by the Stress