13 Table la. Cellular Parameters in Bronchoalveolar Lavage Fluid from Young Rats following Exposure to Combinations of Endotoxin, Carbon, and Ozone Total Cells %AM %PMN %Lymph. %Viable Protein LDH I 13-Glucuronidase (mg/ml) (mg/ml/min} Young Rats: Untreated 0.93 ± 98.87 ± 0.31 ± 0.82 ± 94.84 ± 0.12 ± 33.30 ± 0.24± 0. 10 • 0.95 0.38 0.64 1.85 0.01 • 9.15 0.01 Carbon Only 1.50 ± 98.35 ± 0.48 ± 1.18± 95.02 ± 0.14 ± 42.62 ± 0.39± 0.23 0.68 0.27 0.41 1.80 0.01 3.66 0.08 Ozone Only 1.21 ± 95.39± 2.47 ± 2.14± 94.42 ± 0.40± 60.25 ± 0.35± 0.05 0.15 0.44 0.31 1.86 0.03 14.79 0.00 Carbon/Ozone 1.17 ± 93. IO± 4.05± 2.85 ± 93.33 ± 0.35 ± 60.04 ± 0.33± 0.04 1.72 1.15 0.57 1.57 0.04 26.98 0.02 LPS Only 1.36 ± 88.25 ± 9.47 ± 2.28± 94.85 ± 0.11 ± 35.72 ± 0.21 ± 0.04 2.43 2.06 0.37 0.61 0.01 2.96 0.02 LPS/Carbon 1.45 ± 83.84 ± 14.52 ± 1.64 ± 94.25 ± 0.16± 41.21 ± 0.24± 0.04 2.79 2.70 0.38 0.76 0.00 6.52 0.04 LPS/Ozone 1.36 ± 83.41 ± 13.51 ± 3.08± 94.96± 0.37 ± 43.33 ± - 0.33± 0.11 3.78 3.29 0.60 0.57 0.05 12.12 0.05 LPS/Carbon/ 1.57 ± 80.04 ± 17.43 ± 2.53± 93.16 ± 0.33 ± 95.09± 0.35± Ozone 0.23 2.33 1.98 0.56 1.77 0.04 13.74 0.02 8-55 14 Table lb. Cellular Parameters in Bronchoalveolar Lavage Fluid from Old Rats following Exposure to Combinations of Endotoxin, Carbon, and Ozone Total Cells %AM %PMN %Lymph. %Viable Protein LDH I 13-Glucuronidase (mg/ml) (mg/ml/min) Old Rats: Untreated 2.18 ± 98.70 ± 0.47± 0.83 ± 91.06± 0.17± 49.05 ± 0.35 ± 0.S3 • 0.83 0.30 0.S4 2.66 0.01 18.60 0.08 Carbon Only 1.6S ± 98.26 ± 0.32± l.42 ± 91.59 ± 0.17 ± 26.1S ± 0.2S± 0.24 0.44 0.09 0.41 2.21 0.02 19.38 0.05 OzoneOrily 2.27± 97.82 ± 0.78± 1.40± 90.99± 0.45± 65.43 ± 0.43 ± 0.64 0.26 0.30 0.04 0.42 0.04 9.58 0.09 Carbon/Ozone 2.29± 97.78 ± l.S9± 0.97± 89.88 ± 0.49± 69.44 ± 0.41 ± 0.58 0.77 0.40 0.84 3.75 0.06 5.07 0.03 LPSOnly 1.88 ± 89.91 ± 7.82± 2.27± 88.76 ± 0.21 ± 43.09± 0.30± 0.16 2.71 2.18 0.77 4.58 0.01 19.55 0.02 LPS/Carbon 1.90± 95.33 ± 2.41 ± 2.25± 92.32 ± 0.21 ± 46.92 ± 0.24± 0.58 0.19 0.61 0.75 1.18 0.06 20.51 0.03 LPS/Ozone 2.20± 88.77 ± 8.81 ± 2.41 ± 91.83 ± 0.45± 55.94 ± 0.30± 0.21 3.84 3.52 0.60 0.49 0.09 14.50 0.07 LPS/Carbon/ 2.15 ± 75.62 ± 20.71 ± 3.67± 88.70 ± 0.47± 60.83 ± 0.49± Ozone 0.63 7.61 7.61 1.28 1.63 0.17 4.03 0.24 • Values are reported as means+/- SD; n =3 per group. 8-56 15 Table 2a. Three-Way ANOVAs for Main Effects and Interactions between Factors in Young Rats• Source BAL Resting ROS PMA-Stimulated PMNs (p) ROS (p) (o) Carbon 0.0015 0.0015 0.0519 Ozone <0.0001 0.0013 0.0001 Endo toxin <0.0001 0.0003 <0.0001 Carbon + Ozone 0.9679 0.0148 <0.0001 Endotoxin + Carbon 0.2650 0.0008 0.8071 Endotoxin + Ozone 0.0085 0.0053 0.0066 Endotoxin + Carbon + Ozone 0.4189 0.0087 0.0696 R2 0.966 · 0.855 0.970 Table 2b. Three-Way ANOVAs for Main Effects and Interactions between Factors in Old Rats• Source BALPMNs Resting ROS PMA-Stimulated lo) lo) ROS fol Carbon 0.7470 0.4065 0.3398 Ozone <0.0001 0.0008 <0.0001 Endotoxin <0.0001 <0.0001 <0.0001 Carbon + Ozone 0.0014 0.0086 0.0062 Endotoxin + Carbon 0.2938 0.0017 0.2017 Endotoxin + Ozone 0.9961 0.0742 0.4111 Endotoxin + Carbon + Ozone 0.21 IO 0.0999 0.4895 R2 0.929 0.874 0.933 • The three factors were the presence or absence of carbon, endotoxin, and ozone. 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