TRIM33 Antibody Cat. No.: 7385 TRIM33 Antibody Immunohistochemistry of TRIM33 in human liver tissue Immunofluorescence of TRIM33 in human liver tissue with with TRIM33 antibody at 2.5 μg/ml. TRIM33 antibody at 20 μg/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33. IMMUNOGEN: The immunogen is located within amino acids 580 - 630 of TRIM33. TESTED APPLICATIONS: ELISA, IF, IHC-P, WB TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. September 25, 2021 1 https://www.prosci-inc.com/trim33-antibody-7385.html TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are SPECIFICITY: known to exist. POSITIVE CONTROL: 1) Cat. No. 1304 - Human Liver Tissue Lysate 2) Cat. No. 10-201 - Human Liver Tissue Slide Predicted: 70, 124 kDa PREDICTED MOLECULAR WEIGHT: Observed: 70 kDa Properties PURIFICATION: TRIM33 Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL TRIM33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. Additional Info OFFICIAL SYMBOL: TRIM33 TRIM33 Antibody: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ALTERNATE NAMES: ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7 ACCESSION NO.: NP_056990 PROTEIN GI NO.: 74027249 GENE ID: 51592 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 25, 2021 2 https://www.prosci-inc.com/trim33-antibody-7385.html TRIM33 Antibody: TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and BACKGROUND: bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin- protein ligase and promotes SMAD4 ubiquitination (5). 1) Ransom DG, Bahary N, Niss K, et al. The zebrafish moonshine gene encodes REFERENCES: transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. PLoS Biol. 2004; 2:E237. 2) Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7. 3) He W, Dorn DC, Erdjument-Bromage H, et al. Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. Cell 2006; 125:929-41. 4) Dupont S, Inui M, Newfeld SJ. Regulation of TGF-β signal transduction by mono- and deubiquitylation of Smads. FEBS Lett. 2012; 586:1913-20. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 25, 2021 3 https://www.prosci-inc.com/trim33-antibody-7385.html.