Mouse Rabac1 Knockout Project (CRISPR/Cas9)

<a href="/goto?url=https://www.alphaknockout.com" target="_blank">https://www.alphaknockout.com </a>Mouse Rabac1 Knockout Project (CRISPR/Cas9) Objective: To create a Rabac1 knockout Mouse model (C57BL/6J) by CRISPR/Cas-mediated genome engineering. Strategy summary: The Rabac1 gene (NCBI Reference Sequence: NM_010261 ; Ensembl: ENSMUSG00000003380 ) is located on Mouse chromosome 7. 5 exons are identified, with the ATG start codon in exon 1 and the TAA stop codon in exon 5 (Transcript: ENSMUST00000076961). Exon 1~5 will be selected as target site. Cas9 and gRNA will be co-injected into fertilized eggs for KO Mouse production. The pups will be genotyped by PCR followed by sequencing analysis. Note: Exon 1 starts from about 0.18% of the coding region. Exon 1~5 covers 100.0% of the coding region. The size of effective KO region: ~2657 bp. The KO region does not have any other known gene. Page 1 of 8 <a href="/goto?url=https://www.alphaknockout.com" target="_blank">https://www.alphaknockout.com </a>Overview of the Targeting Strategy Wildtype allele <ul style="display: flex;"><li style="flex:1">gRNA region </li><li style="flex:1">gRNA region </li></ul><ul style="display: flex;"><li style="flex:1">5' </li><li style="flex:1">3' </li></ul><ul style="display: flex;"><li style="flex:1">1</li><li style="flex:1">2</li><li style="flex:1">3</li><li style="flex:1">4</li><li style="flex:1">5</li></ul>Legends <ul style="display: flex;"><li style="flex:1">Exon of mouse Rabac1 </li><li style="flex:1">Knockout region </li></ul>Page 2 of 8 <a href="/goto?url=https://www.alphaknockout.com" target="_blank">https://www.alphaknockout.com </a>Overview of the Dot Plot (up) Window size: 15 bp <ul style="display: flex;"><li style="flex:1">Forward </li><li style="flex:1">Reverse Complement </li></ul>Note: The 2000 bp section upstream of start codon is aligned with itself to determine if there are tandem repeats. No significant tandem repeat is found in the dot plot matrix. So this region is suitable for PCR screening or sequencing analysis. Overview of the Dot Plot (down) Window size: 15 bp <ul style="display: flex;"><li style="flex:1">Forward </li><li style="flex:1">Reverse Complement </li></ul>Note: The 2000 bp section downstream of stop codon is aligned with itself to determine if there are tandem repeats. Tandem repeats are found in the dot plot matrix. The gRNA site is selected outside of these tandem repeats. Page 3 of 8 <a href="/goto?url=https://www.alphaknockout.com" target="_blank">https://www.alphaknockout.com </a>Overview of the GC Content Distribution (up) Window size: 300 bp Summary: Full Length(2000bp) | A(25.65% 513) | C(27.7% 554) | T(25.3% 506) | G(21.35% 427) Note: The 2000 bp section upstream of start codon is analyzed to determine the GC content. No significant high GC-content region is found. So this region is suitable for PCR screening or sequencing analysis. Overview of the GC Content Distribution (down) Window size: 300 bp Summary: Full Length(2000bp) | A(24.65% 493) | C(26.35% 527) | T(22.45% 449) | G(26.55% 531) Note: The 2000 bp section downstream of stop codon is analyzed to determine the GC content. No significant high GC-content region is found. So this region is suitable for PCR screening or sequencing analysis. Page 4 of 8 <a href="/goto?url=https://www.alphaknockout.com" target="_blank">https://www.alphaknockout.com </a>BLAT Search Results (up) QUERY SCORE START END QSIZE IDENTITY CHROM -------------------------------------------------------------------------------------------------------------- <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr7:24972583-24974582&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=24972582&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr7&l=24972582&r=24974582&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq 2000 1 2000 2000 100.0% chr7 24972583 24974582 2000 + 126146270 126146408 83003775 83003907 <ul style="display: flex;"><li style="flex:1">STRAND START </li><li style="flex:1">END SPAN </li></ul>-<a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr10:126146270-126146408&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=126146269&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr10&l=126146269&r=126146408&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr3:83003775-83003907&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=83003774&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr3&l=83003774&r=83003907&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr4:103181280-103181412&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=103181279&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr4&l=103181279&r=103181412&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr8:128708275-128708410&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=128708274&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr8&l=128708274&r=128708410&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr4:118327394-118327527&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=118327393&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr4&l=118327393&r=118327527&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:62109451-62109573&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=62109450&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr11&l=62109450&r=62109573&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr1:157422345-157422537&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=157422344&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr1&l=157422344&r=157422537&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr14:20983029-20983161&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=20983028&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr14&l=20983028&r=20983161&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr2:132207057-132207171&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=132207056&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr2&l=132207056&r=132207171&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr12:25151378-25151509&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=25151377&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr12&l=25151377&r=25151509&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chrX:7636566-7636697&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=7636565&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chrX&l=7636565&r=7636697&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr7:126222317-126222448&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=126222316&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr7&l=126222316&r=126222448&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:6197536-6197676&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=6197535&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr11&l=6197535&r=6197676&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr4:118966270-118966393&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=118966269&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr4&l=118966269&r=118966393&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr18:32076461-32076582&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=32076460&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr18&l=32076460&r=32076582&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr5:128062550-128062681&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=128062549&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr5&l=128062549&r=128062681&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:120637935-120638060&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=120637934&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr11&l=120637934&r=120638060&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr12:54809763-54814142&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=54809762&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr12&l=54809762&r=54814142&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:102966181-102966294&db=mm10&ss=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+../trash/hgSs/hgSs_genome_6b539_4c31f0.fa&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=102966180&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b539_4c31f0.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b539_4c31f0.fa+YourSeq&c=chr11&l=102966180&r=102966294&db=mm10&hgsid=796030441_m6n6wOcFQhFxAzkr7vwjR5lJp4lM" target="_blank">details </a>YourSeq 98 95 89 88 88 88 88 87 86 85 85 84 84 84 83 83 83 82 82 3 147 2000 3 136 2000 2 134 2000 7 144 2000 1 134 2000 2 121 2000 2 131 2000 2 132 2000 14 129 2000 2 134 2000 14 143 2000 2 134 2000 7 143 2000 8 131 2000 1 121 2000 5 134 2000 2 126 2000 21 125 2000 8 121 2000 80.6% chr10 84.7% chr3 83.5% chr4 82.6% chr8 82.9% chr4 89.4% chr11 87.1% chr1 84.3% chr14 85.3% chr2 85.4% chr12 83.1% chrX 88.5% chr7 82.9% chr11 83.9% chr4 84.9% chr18 82.2% chr5 83.8% chr11 89.6% chr12 86.0% chr11 139 133 133 136 134 123 193 133 115 132 132 132 141 124 122 132 126 -- 103181280 103181412 - 128708275 128708410 - 118327394 118327527 <ul style="display: flex;"><li style="flex:1">-</li><li style="flex:1">62109451 62109573 </li></ul>- 157422345 157422537 20983029 20983161 - 132207057 132207171 --+ 25151378 25151509 7636566 7636697 - 126222317 126222448 6197536 6197676 + 118966270 118966393 32076461 32076582 --+ 128062550 128062681 + 120637935 120638060 <ul style="display: flex;"><li style="flex:1">-</li><li style="flex:1">54809763 54814142 4380 </li></ul>- 102966181 102966294 114 Note: The 2000 bp section upstream of start codon is BLAT searched against the genome. No significant similarity is found. BLAT Search Results (down) <ul style="display: flex;"><li style="flex:1">QUERY SCORE START END QSIZE IDENTITY CHROM STRAND START </li><li style="flex:1">END SPAN </li></ul>----------------------------------------------------------------------------------------------- <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr7:24967924-24969923&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=24967923&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr7&l=24967923&r=24969923&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq 2000 <ul style="display: flex;"><li style="flex:1">1 2000 2000 100.0% chr7 - </li><li style="flex:1">24967924 24969923 2000 </li></ul>63000526 63327033 326508 44023490 44287514 264025 <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr10:63000526-63327033&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=63000525&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr10&l=63000525&r=63327033&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq 272 237 1652 2000 <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr10:44023490-44287514&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=44023489&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr10&l=44023489&r=44287514&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq 138 265 871 2000 <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr10:128403396-128403794&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=128403395&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr10&l=128403395&r=128403794&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq 115 237 491 2000 <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:96564165-96898566&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=96564164&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr11&l=96564164&r=96898566&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq 108 239 900 2000 <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:85175090-85175332&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=85175089&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr11&l=85175089&r=85175332&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq 102 240 481 2000 <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr8:25502961-25503526&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=25502960&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr8&l=25502960&r=25503526&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq 101 236 478 2000 92.0% chr10 - 84.5% chr10 - <ul style="display: flex;"><li style="flex:1">86.2% chr10 + 128403396 128403794 </li><li style="flex:1">399 </li></ul>87.5% chr11 + 84.9% chr11 - 85.9% chr8 - 84.1% chr7 - 96564165 96898566 334402 85175090 85175332 25502961 25503526 34318815 34319190 243 566 376 214 567 288 372 301 318 487 <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr7:34318815-34319190&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=34318814&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr7&l=34318814&r=34319190&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr8:121845959-121846172&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=121845958&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr8&l=121845958&r=121846172&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr6:72639364-72639930&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=72639363&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr6&l=72639363&r=72639930&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr1:160861625-160861912&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=160861624&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr1&l=160861624&r=160861912&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:57699822-57700193&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=57699821&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr11&l=57699821&r=57700193&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr1:91432401-91432701&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=91432400&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr1&l=91432400&r=91432701&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr12:102298612-102298929&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=102298611&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr12&l=102298611&r=102298929&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr9:44269215-44269701&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=44269214&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr9&l=44269214&r=44269701&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr11:20364331-20751636&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=20364330&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr11&l=20364330&r=20751636&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr17:29114493-29114603&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=29114492&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr17&l=29114492&r=29114603&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr14:34215295-34215376&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=34215294&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr14&l=34215294&r=34215376&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr5:128372364-128372461&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=128372363&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr5&l=128372363&r=128372461&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq <a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgTracks?position=chr7:28190176-28190247&db=mm10&ss=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+../trash/hgSs/hgSs_genome_6b5c5_4c3360.fa&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">browser </a><a href="/goto?url=https://genome.ucsc.edu/cgi-bin/hgc?o=28190175&g=htcUserAli&i=../trash/hgSs/hgSs_genome_6b5c5_4c3360.pslx+..%2Ftrash%2FhgSs%2FhgSs_genome_6b5c5_4c3360.fa+YourSeq&c=chr7&l=28190175&r=28190247&db=mm10&hgsid=796030549_AT2RgooyZqJnIanic0jGSuwy3aSc" target="_blank">details </a>YourSeq

Mouse Rabac1 Knockout Project (CRISPR/Cas9)

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