Leader in Biomolecular Solutions for Life Science WASL Knockout HeLa Cell Lysate Catalog No.: RM02299 Basic Information Background Catalog No. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott- RM02299 Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed Category in neural tissues, and interacts with several proteins involved in cytoskeletal organization, Cell Lysate including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013] Description WASL Knockout HeLa Cell Line is engineered from HeLa cell line with CRISPR/Cas9 technology. Allele-1:1bp insertion in exon1 Allele-2:4bp deletion in exon1 Gene Information Product Information Gene Symbol Parental Cell line Amount Genotype WASL HeLa 50μL, 2μg/μL. Heterozygous knockout Species Packaging Human 1 vial parental cell Lysate and 1 vial knockout cell Lysate Gene ID Shipping Conditions 8976 4℃ Swiss Prot Application O00401 Western Blot Synonyms Storage N-WASP, NWASP, WASPB Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles. Protocol Contact To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). 400-999-6126 Lysate should be boiled for 3 - 5 minutes before loading onto gel. [email protected] www.abclonal.com.cn Sequencing data Genome sequence analysis of PCR products from parental (WT) and WASL knockout (KO) HeLa cells, using sanger sequencing. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..