Executive Organizing Committee Mariana Legu´ıa(Chair) Pontificia Universidad Cat´olicadel Per´u,Lima, Per´u Carlos Sariol (Secretary) University of Puerto Rico, PR, USA Mark Challberg National Institutes of Health, MD, USA George Dimopoulos Johns Hopkins University, MD, USA Andrea Gamarnik Fundaci´onInstituto Leloir, Buenos Aires, Argentina Eva Harris University of California-Berkeley, CA, USA Amy C. Morrison University of California-Davis, CA, USA Mauricio L. Nogueira Faculdade de Medicina de S~aoJos´edo Rio Preto, Brazil Valerie Paz-Sold´an Universidad Peruana Cayetano Heredia, Lima, Per´u Tulane University, LA, USA Nikolaos Vasilakis University of Texas Medical Branch, TX, USA Gissella Vasquez U.S. Naval Medical Research Unit No. 6, Lima, Per´u Daniela Weiskopf La Jolla Institute for Immunology, CA, USA Peruvian Organizing Committee Mariana Legu´ıa(Chair) Directora, Laboratorio de Gen´omica Pontificia Universidad Cat´olicadel Per´u Vilma R. Bejar Directora, Instituto de Medicina Tropical Daniel A. Carri´on Universidad Nacional Mayor de San Marcos C´esar Cabezas Jefe, Instituto Nacional de Salud Profesor, Universidad Nacional Mayor de San Marcos M´aximoM. Espinoza Presidente, Sociedad Peruana de Enfermedades Infecciosas y Tropicales Theresa J. Ochoa Directora, Instituto de Medicina Tropical Alexander von Humboldt Universidad Peruana Cayetano Heredia Valerie Paz-Sold´an Profesora, Universidad Peruana Cayetano Heredia Profesora, Tulane University Gissella V´asquez Sub Jefa, Departamento de Entomolog´ıa U.S. Naval Medical Research Unit No. 6 Hans D. V´asquez Ex Jefe, Instituto Nacional de Salud Scientific Committee Andrea Gamarnik (Chair) Fundaci´onInstituto Leloir, Buenos Aires, Argentina Eva Harris (Co-Chair) University of California-Berkeley, CA, USA Sub-Committees Virology/Pathogenesis/Antivirals Diagnostics/Prognostics/Clinical Andrea Gamarik (Chair) Jorge Mu~noz(Chair) Rosa Mar´ıadel Angel´ (Co-Chair) Luisa Alvarado (Co-Chair) Andrea da Pollan Patricia Brasil Elsa Damonte Paquita Garc´ıa Mauro Teixeira Susan Wong Epi/Phylogenetics/Modeling/ Vector Biology/Ecology/Control Burden Amy C. Morrison (Chair) Albert Ko (Chair) George Dimopolous (Co-Chair) Mauricio L. Nogueira (Co-Chair) Roberto Barrera Nate Grubaugh Luciano Moreira Mariana Legu´ıa Nikos Vasilakis Isabel Rodriguez Gissella V´asquez Diana Rojas Tyler Sharp Immunology/Vaccines Eva Harris (Chair) Human Behavior & Community Ana Sesma (Co-Chair) Engagement Aravinda de Silva Josefina Coloma (Chair) Daniela Weiskopf Valerie Paz-Sold´an(Co-Chair) Steve Whitehead Table of Contents Oral Presentations...................................1 Diagnostics-Prognostics-Clinical.......................1 Epi-Phylogenetics-Modeling-Burden.................4 Human Behavior & Community Engagement....7 Immunology-Vaccines.......................................9 Vector Biology-Ecology-Control.......................13 Virology-Pathogenesis-Antivirals......................16 Highlighted Posters.................................21 Diagnostics-Prognostics-Clinical.......................21 Epi-Phylogenetics-Modeling-Burden.................24 Human Behavior & Community Engagement...27 Immunology-Vaccines......................................28 Vector Biology-Ecology-Control.......................30 Virology-Pathogenesis-Antivirals......................32 Posters....................................................36 Diagnostics-Prognostics-Clinical.......................36 Epi-Phylogenetics-Modeling-Burden.................69 Human Behavior & Community Engagement....91 Immunology-Vaccines.......................................94 Vector Biology-Ecology-Control......................110 Virology-Pathogenesis-Antivirals.....................124 Late Breaker Abstracts...........................146 Author Index..........................................165 Oral Presentations Diagnostics-Prognostics- nificantly higher ratio of plasma ZIKV/DENV1-4 Clinical NS1 IgG than mothers without suspected ZIKV infection and IgG-ZIKV was higher in mothers that their children. When mothers and progeny 1 were match, the levels of anti-NS1-ZIKV IgG was significantly higher in those that developed CZS High and specific Humoral maternal- that mothers exposed to ZIKV but children with- and-child response to NS1 correlates out CZS. We propose a transplacental movement with long-term neurological effects of ZIKV NS1-IgG to explain the correlation found during prenatal Zika virus exposure in virus-specific IgG between mothers and chil- dren. Levels of ZIKV NS1-IgG were transient Doris M. Salgado1,2, Jos´e S. Cort´es1, William as they fall to undetectable in children older 6 Mej´ıa1,2, Roc´ıo Vega1,2, Angela Ort´ız1,2, Irene months. Neurological follow-up was done in 104 Bosch3,4, Isabel DeLaura1,5, Lee Gehrke6,7, Car- infants, including 17 patients with microcephaly, los F. Narv´aez1,2 10 with ocular abnormalities and 77 children with- 1Divisi´onde Inmunolog´ıa y Pediatr´ıa, Programa de out CZS. 16% of the children prenatally exposed Medicina, Facultad de Salud, Universidad Surcolom- to ZIKV without CZS presented other cognitive biana, Neiva, Huila, Colombia; 2Servicio de Pediatr´ıa, compromise, 15% motor and 6% of the language Hospital Universitario de Neiva, Neiva, Huila, Colom- impairment. Thus, ZIKV prenatal infections in- bia; 3E25Bio Inc., The Engine of MIT, MA, USA; duced a virus-specific humoral response with po- 4Department of Medicine, Mount Sinai School of tential trans-placenta movement and correlated in Medicine, NY, USA; 5School of Medicine, Duke Uni- magnitude with infection. Additionally, prenatal versity, Durham, NC, USA; 6Massachusetts Institute infection of ZIKV results in a high frequency of of Technology, Cambridge, MA, USA; 7Department of cognitive disorders that are uncover upon neuro- Microbiology, Harvard Medical School, Boston, MA, logical follow-up. USA Humoral responses play a critical role in the pro- 2 tection and pathogenesis of the Zika virus (ZIKV) infection and its congenital consequences. To Clinical and epidemiological charac- determine the pre-exposure to ZIKV in high-risk terization of orthobunyavirus associ- populations such as pregnant women and children ated with febrile disease in humans in through their virus-specific antibody response is the Peruvian Amazon crucial. Due to the high number of asymptomatic 1 1 infections and cross-reactive responses among co- Crystyan Siles , Stalin Vilcarromero , Helvio 1 2 1,3 circulating Flaviviruses, screening for prior expo- Astete , Carlos Alvarez , Amy C. Morrison , 1 1 sure is a challenge. Here, we analyzed the circulat- Carolina Guevara , Eugenio Abente , Julia S. Am- 1 ing ZIKV and dengue virus (DENV)-specific IgG, puero and the neurodevelopment in 104 symptomatic 1U.S. Naval Medical Research Unit No.6, Iquitos and mothers admitted to the Hospital Universitario Lima, Per´u; 2Direcci´onRegional de Salud, Loreto, de Neiva, Colombia and the progeny exposed to Per´u; 3University of California, Davis, CA, USA ZIKV in utero, during the epidemic year. There Introduction: Emerging infectious diseases can were 17 newborns diagnosed with congenital zika negatively impact medical readiness of the US mil- syndrome (CZS) in this cohort. A NS1-based in- itary and continued surveillance is warranted to direct ELISA was used to evaluate levels of ZIKV support Force Health Protection. Viruses from and DENV-specific IgG. A 12 months neurologi- the genus Orthobunyavirus circulate globally in cal follow-up including the specific Bayley III scale tropical, temperate and arctic ecological niches evaluating the motor, language, and cognitive as- and include Oropouche (OROV) Guaroa (GROV) pects, was applied to all children. The ZIKV and viruses from the group C serogroup (GRCV). IgG NS1-based ELISA shows a low cross-reactivity Objectives: To characterize the clinical and epi- with DENV when samples of children with con- demiological aspects of orthobunyaviruses associ- firmed primary or secondary DENV infection were ated with febrile disease in humans. Methods: tested. Mothers with ZIKV infections shown a sig- Subjects no less than 5 years old with fever no 1 less than 5 days in duration who sought medi- y Referencia, Ministry of Health, Managua, Nicaragua cal attention in urban, peri-urban and rural health 5Department of Epidemiology, School of Public facilities in Iquitos, Peru were invited to partici- Health, University of Michigan, Ann Arbor, MI, USA pate in an IRB-approved clinic-based surveillance 6Centro de Salud S´ocratesFlores Vivas, Ministry of study. Acute and convalescent blood sample were Health, Managua, Nicaragua collected. Samples were screened for evidence Introduction. While Zika in adults presents as a of viral infection by RT-PCR and/or viral isola- mild, dengue-like disease, pediatric Zika remains tion and/or a 4-fold increase in IgM antibodies an understudied topic. The World Health Orga- as tested by ELISA in paired samples. Results: nization (WHO) and Pan American Health Orga- 2448/8395 samples collected between December nization (PAHO) Zika case definitions have not 2010 and May 2016 were positive for an arbovirus: been assessed in children. Objective.We aimed flaviviruses (n=2308), alphaviruses (n=94) and to characterize clinical profiles and evaluate the orthobunyaviruses (n=46). Among the orthobun- diagnostic performance of the WHO and PAHO yaviruses found, 20 were GROV, 17 were GRCV case definitions in a large cohort of pediatric Zika and 9 were OROV. Of the 46 positive-cases for an cases. Materials and methods. We prospectively orthobunyavirus, 50% were
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