Vacutainer Tube Guide - Order of Draw for routine volumes See separate guide for micro-tainers Number of Tube Inversions at Label Blood Collection Volume Tubes/Bottles Abbrev. Additive (Do Not Shake) General Laboratory Use Draw 1 Aerobic and 1 5 Blood Culture / requires Adapter 20 mL Anaerobic Culture Note: A separate venipuncture for trace BLCLT Bottles metal analysis is required if blood cultures are ordered at the same time. NAYYP Clot activator 8 Trace Element serum tube for Copper 6 mL Royal Trace Element whole blood for Aluminum, NAVYE 2 Call 2-5002 Blue K EDTA Lead, Zinc, Selenium, Manganese, Mercury, 8 for tubes Cadmium, Arsenic Discard tube or secondary sterile specimen 6 mL Clear None 0 tube 3 mL 2.7 mL Light (Blue) BLUE Sodium Citrate (3.2%) Coagulation Testing on Plasma Blue 3-4 1.8 mL (Blue/clear) Serum determinations where a gel-barrier is 6 mL Red RED Clot Activator 5 contraindicated; Therapeutic Drug 4 mL Monitoring (TDM) Clot Activator and gel SST - Serum determinations in Chemistry, Gold SST 5 5 mL for serum separation Immunology, Serology; HLA Ab screen, DSA Light Lithium heparin and PST PST - Plasma determinations in Chemistry 4.5 mL Green gel - plasma separation 8 GLI Lithium heparin 8 Whole Blood Plasma: Troponin, Lactic Acid, Ion. Ca+ 6 mL Dark 4 mL Green GNA Sodium heparin 8 Whole Blood Plasma: Tissue typing, plasma hemoglobin, plasma catecholamine, chromosome analysis Pink PINK Dry K2EDTA 8 Blood Bank / Transfusion; HLA Typing 6 mL Tan TAN K2EDTA 8 Lead Testing only 3 mL LAV 4 Whole Blood Hematology; some Flow, 4 mL Lavender Dry K2EDTA 8 Molecular, and Genetic tests; HIV Viral 2 mL LAV6 Load Whole Blood tube with Glycolytic inhibitor Potassium Oxalate / for GTT, Gray 6 mL GRAY Sodium fluoride 8 Post-Prandial Glucose, D-Xylose, and Volatiles panel ACDA ACD Whole Blood tube for Flow Yellow (Acid Citrate Dextrose 8 immunophenotyping; HLA cross matching; 8.5 mL ACDB Solution A) genetic and other referred tests ALWAYS Check Expiration Dates before use! If unsure of use, refer to the on-line “Lab User’s Guide” or call the lab at 25002 .