August 13-14, 2015 Fred Hutchinson Cancer Research Center Seattle, Washington cgt4hivcure2015.org COMMUNITY EVENT Seattle, August 13-14 TABLE OF CONTENTS Welcome........................................................................................ 2 Scholarship Recipients .............................................................4 Scientific Organizing Committee ......................................... 5 Agenda ........................................................................................... 6 Keynote .......................................................................................... 8 Plenary Speakers ...................................................................... 10 Speakers (by session) ..............................................................14 Poster Presentation Abstracts ............................................43 Host Organizations ..................................................................58 Dinner and Reception .............................................................62 Sponsors ..................................................................................... 64 Community Representation and Financial Support .. 68 Conference on Cell & Gene Therapy for HIV Cure 2015 1 WELCOME On behalf of the Scientific Organizing Committee, we are pleased to welcome you to the 2nd annual Conference on Cell & Gene Therapy for HIV Cure 2015. It is a privilege to host this year’s event and we are proud to showcase the cutting-edge research in cell and gene therapy that has brought us closer than ever to finding a cure for HIV. For the second year running, we are holding this conference at Fred Hutchinson Cancer Research Center, a fitting location for a conference established on the notion of finding a cure for HIV. A significant proportion of HIV-related gene therapy research builds directly off the success and refinement of bone marrow transplants, a technique developed by Fred Hutch’s own E. Donnall Thomas. Bone marrow transplantation has been used to effectively treat many diseases such as leukemia and lymphoma, and in 2007/2008, an allogenic bone marrow transplant was utilized to produce the first and only known cure of HIV. This single case gave gene therapy a tremendous boost, and legitimized the pursuit of an HIV cure. 2 Seattle, August 13-14 WELCOME Today’s HIV treatments focus primarily on controlling the disease and mitigating its burden on those infected so that patients carrying the virus can live long productive lives. This form of therapy, while effective, has significant drawbacks with regard to cost, side effects, and lingering presence of the virus that is never fully eliminated. Recent advancements in the gene therapy, transplantation and immunology fields have made HIV cure research a reality - a decade ago this goal was merely visionary. In the spirit of the Fred Hutch slogan, “Cures Start Here”, we intend for this meeting to foster the collaborations and produce the science that will bring us all one step closer to reaching that ultimate goal. Thank you all for your participation at this conference, and we hope you find the program engaging and informative in a way that will advance your own HIV research and advocacy. Keith R. Jerome, MD, PhD Hans-Peter Kiem, MD Co-PI, defeatHIV Co-PI, defeatHIV Conference on Cell & Gene Therapy for HIV Cure 2015 3 SCHOLARSHIP RECIPIENTS The Conference on Cell & Gene Therapy for HIV Cure is pleased to announce the 2015 scholarship recipients: Biswajit Paul Paul Munson Mayumi Takahashi, PhD Pavitra Roychoudhury, PhD Sangeetha Satheesan, MSc Nixon Niyonzima, MD, MSc. Melanie Alvarado Mesfin Gewe, PhD Joumana Zeidan, PhD Congratulations! 4 Seattle, August 13-14 SCIENTIFIC ORGANIZING COMMITTEE Galit Alter, PhD Ronald Mitsuyasu, MD Associate Professor of Medicine, Kristine Professor, Department of Medicine, and Bob Higgins MGH Research Scholar, Director, Clinical AIDS Research and Ragon Institute of MGH, MIT and Harvard Education Center (CARE), University of California Los Angeles Paula Cannon, PhD defeatHIV Scientific Advisory Panel Associate Professor, Molecular Microbiology & Immunology, Pediatrics, Julie M Overbaugh, PhD Biochemistry & Molecular Biology, Keck School of Medicine , University of Member, Human Biology Division and Southern California Public Health Sciences Division, Fred Hutchinson Cancer Research Center defeatHIV Steering Committee Erick Seelbach Keith R. Jerome, MD, PhD HIV/AIDS Regional Resource Consultant, Host, Conference on CGT4HIVCure2015 U.S. Department of Health and Human Co-PI, defeatHIV Martin Delaney Services Collaboratory defeatHIV and CFAR Community Member, Vaccine and Infectious Disease Advisory Boards” Division, Fred Hutchinson Cancer Research Center Geoff Symonds PhD Professor and Head, Virology Division, Chief Scientific Officer, Calimmune, Inc. Dept of Laboratory Medicine University of Washington Jerome Zack, PhD Hans-Peter Kiem, MD Professor, Microbiology, Immunology And Molecular Genetics, David Geffen School Host, Conference on CGT4HIVCure2015 of Medicine, PI, UCLA CFAR, University of Co-PI, defeatHIV Martin Delaney California Los Angeles Collaboratory Member, Clinical Research Division, Affiliate Investigator, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center Associate Head, Heme Malignancy Program, UW / Fred Hutch Cancer Consortium Professor of Medicine / Adjunct Professor of Pathology, University of Washington School of Medicine Conference on Cell & Gene Therapy for HIV Cure 2015 5 AGENDA | DAY 1: 7:00-8:00 Registration and Continental Breakfast SESSION 1 Opening Remarks & HIV Cure Overview Conference Welcome: Keith R. Jerome, MD, PhD; 8:00-8:15 Hans-Peter Kiem, MD 8:15-8:45 Pursuit of an HIV Cure: Jerome Zack, PhD SESSION 2 HCT for HIV Cure 8:45-9:15 Richard Ambinder, MD, PhD 9:15-9:35 Timothy Henrich, MD 9:35-9:55 Leslie Kean, MD, PhD 9:55-10:15 Break SESSION 3 Keynote 10:15-10:30 Keynote Introduction: Lawrence Corey, MD 10:30-11:30 Keynote Speaker: David Baltimore, PhD 11:30-12:30 Lunch: Weintraub B-Suites 12:30-1:00 Plenary Speaker: Matthew Porteus, MD, PhD SESSION 4 Gene Editing 1:00-1:30 Paula Cannon, PhD 1:30-1:45 Andrew Scharenberg, MD Pelton Auditorium Pelton 1:45-2:00 Daniel Stone, PhD 2:00-2:15 Biswajit Paul 2:15-2:30 Christopher Peterson, PhD 2:30-2:45 Break SESSION 5 CAR T Cell Development for HIV Cure 2:45-3:15 Lawrence Corey, MD 3:15-3:30 Thor Wagner, MD 3:30-3:45 Guillermo Romano-Ibarra 3:45-4:00 Malika Hale 4:00-4:15 Masakazu Kamata, PhD SESSION 6 Cure in the Community 4:15-4:30 Jessica Handibode, MBE 4:30-4:45 Michele Andrasik, PhD Evening Activities Poster Session, Cocktails and Hors d ’Oeuvres 5:00-6:30 (SZE conference room) 6:30-7:00 Shuttle from Fred Hutch to Space Needle 7:00-10:00 Dinner Reception – Space Needle (downtown Seattle) 6 Seattle, August 13-14 DAY 2: | AGENDA 7:30-8:00 Registration and Continental Breakfast 8:00-8:30 Plenary Speaker: Dan Barouch, MD, PhD SESSION 7 Vaccines & Immunity 8:30-9:00 James Mullins PhD 9:00-9:15 Julie Overbaugh, PhD 9:15-9:30 Paul Munson 9:30-9:45 R. Keith Reeves, PhD 9:45-10:00 Break 10:00-10:30 Plenary Speaker: Michael Farzan, PhD SESSION 8 HIV Latency and Reservoirs 10:30-11:00 Rafick-Pierre Sékaly, PhD 11:00-11:30 Jerome Zack, PhD 11:30-11:45 Matthew Strain, MD, PhD 11:45-12:00 Joumana Zeidan, PhD Pelton Auditorium Pelton 12:00-1:00 Lunch: Weintraub B-Suites SESSION 9 Therapeutic Development & Gene Therapy 1:00-1:30 Romas Geleziunas, PhD, Gilead 1:30-2:00 Jeffrey Bartlett, PhD, Calimmune 2:00-2:15 Jennifer E. Adair, PhD 2:15-2:30 Dong Sung An, MD, PhD 2:30-3:00 Michael Holmes, PhD, Sangamo Closing Remarks: Keith R. Jerome, MD, PhD; 3:00 - Hans-Peter Kiem, MD The FHCRC offers free wireless access via the FHCRC Guest Network. Conference on Cell & Gene Therapy for HIV Cure 2015 7 KEYNOTE SPEAKER David Baltimore, PhD President Emeritus; Robert Andrews Millikan Professor of Biology California Institute of Technology 1975 Nobel Prize in Physiology or Medicine David Baltimore, former president of the California Institute of Technology (1997-2006) is President Emeritus and the Robert Andrews Millikan Professor of Biology. He is an accomplished researcher, educator, administrator and public advocate for science and engineering and is considered one of the world’s most influential biologists. He received his BA in Chemistry from Swarthmore College in 1960 and a PhD in 1964 from Rockefeller University. Awarded the Nobel Prize in 1975 in Physiology or Medicine for his research into viral replication that provided the key to understanding the life cycle of retroviruses, Baltimore has profoundly influenced national science policy on such issues as recombinant DNA research and the AIDS epidemic. 8 Seattle, August 13-14 KEYNOTE SPEAKER His present research focuses on control of inflammatory and immune responses as well as on the use of gene therapy methods to treat HIV and cancer in a program called “Engineering Immunity”. In addition, he co-directs the Joint Center for Translational Medicine, an activity that joins Caltech and UCLA in a program to translate basic science discoveries into clinical realities. Baltimore’s numerous honors include the 1999 National Medal of Science and 2000 Warren Alpert Foundation Prize. He is past-President and Chair of the American Association of the Advancement of Science and has published more than 680 peer-reviewed articles. Engineering Immunity D Baltimore California Institute of Technology, Pasadena, CA Our immune system does a fine job protecting us against a range of