Phase 3 Field Guide – Ozone Bioindicator Plants (combined), Version 5.1 October, 2011 Section 20. Ozone Bioindicator Plants 20.1 Overview .............................................................................................................................................. 3 20.1.1 Scope and Application ................................................................................................................ 3 20.1.2 Summary of Method .................................................................................................................... 4 20.1.3 Summary of PDR Screens and Tally Procedures ....................................................................... 4 20.1.4 Equipment And Supplies ............................................................................................................. 4 20.1.5 Training and Quality Assurance .................................................................................................. 5 20.1.6 Voucher Specimens .................................................................................................................... 5 20.1.7 Communications ......................................................................................................................... 5 20.2 Ozone Biomonitoring Procedures ..................................................................................................... 7 20.2.1 Evaluation Window ...................................................................................................................... 7 20.2.2 Site Selection Procedures ........................................................................................................... 7 20.2.3 Site Mapping ............................................................................................................................... 8 20.2.4 Split Plots .................................................................................................................................... 9 20.2.5 Species Selection ...................................................................................................................... 10 20.2.6 Plant Selection .......................................................................................................................... 12 20.2.7 Symptom Identification and Scoring .......................................................................................... 13 20.2.8 Collection of Leaf Samples and Voucher Data ......................................................................... 15 20.2.9 Voucher Mailing Procedures ..................................................................................................... 18 20.2.10 Crew Member Responsibilities .............................................................................................. 18 20.2.11 Field Procedures for Untrained Field Crews .......................................................................... 18 20.3 Site Intensification ........................................................................................................................... 19 20.4 Plot Level Data .................................................................................................................................. 19 20.4.1 STATE ...................................................................................................................................... 19 20.4.2 COUNTY .................................................................................................................................. 19 20.4.3 FIELD ID .................................................................................................................................. 20 20.4.4 SPLITPLOT ID ......................................................................................................................... 20 20.4.5 QA STATUS ............................................................................................................................. 20 20.4.6 CREW TYPE ............................................................................................................................ 20 20.4.7 OZONE SAMPLE KIND ........................................................................................................... 21 20.4.8 CURRENT DATE ...................................................................................................................... 21 20.4.8.1 YEAR ................................................................................................................................. 21 20.4.8.2 MONTH .............................................................................................................................. 21 20.4.8.3 DAY .................................................................................................................................... 21 20.4.9 PLOT SIZE ............................................................................................................................... 22 20.4.10 ASPECT ............................................................................................................................... 22 20.4.11 TERRAIN POSITION ............................................................................................................ 22 20.4.12 SOIL DEPTH ........................................................................................................................ 22 20.4.13 SOIL DRAINAGE (East only) ............................................................................................... 23 20.4.13 PLOT WETNESS (West only) .............................................................................................. 23 20.4.14 DISTURBANCE .................................................................................................................... 23 20.4.15 INJURY CHECK ................................................................................................................... 23 20.4.16 ELEVATION ......................................................................................................................... 24 20.4.17 Plot Notes .................................................................................................................................. 24 20.4.17.1 REMARK1 and REMARK2 ............................................................................................. 24 20.5 GPS Coordinates .............................................................................................................................. 24 20.5.1 GPS Unit Settings, Datum, and COORDINATE SYSTEM ........................................................ 25 20.5.2 Collecting Readings .................................................................................................................. 25 20.5.3 GPS UNIT ................................................................................................................................. 25 20.5.4 GPS SERIAL NUMBER ............................................................................................................ 25 20.5.5 GPS ENTRY METHOD ............................................................................................................. 26 20.5.6 GPS DATUM ............................................................................................................................. 26 20.5.7 Latitude...................................................................................................................................... 26 20.5.7.1 LATITUDE DEGREES ....................................................................................................... 26 1 Phase 3 Field Guide – Ozone Bioindicator Plants (combined), Version 5.1 October, 2011 20.5.7.2 LATITUDE MINUTES ........................................................................................................ 27 20.5.7.3 LATITUDE SECONDS ....................................................................................................... 27 20.5.8 Longitude................................................................................................................................... 27 20.5.8.1 LONGITUDE DEGREES ................................................................................................... 27 20.5.8.2 LONGITUDE MINUTES ..................................................................................................... 27 20.5.8.3 LONGITUDE SECONDS ................................................................................................... 28 20.5.9 GPS ELEVATION ..................................................................................................................... 28 20.5.10 GPS ERROR ......................................................................................................................... 28 20.5.11 NUMBER OF GPS READINGS ............................................................................................. 28 20.5.12 GPS FILENAME (CORE OPTIONAL) ................................................................................... 28 20.6 Foliar Injury Data ........................................................................................................................ 29 20.6.1 SPECIES .................................................................................................................................. 29 20.6.2 AMOUNT .................................................................................................................................. 29 20.6.3 NUMBER OF PLANTS ............................................................................................................