SUPPLEMENTAL FIGURE COLON p53 EXONIC STRAND BREAKS DURING FOLATE DEPLETION-REPLETION INTERVENTION Supplemental Figure Legend Strand breaks for p53 exon 6 and 8 among different time course of folate depletion or repletion in the rectosigmoid mucosa. The input of DNA was controlled by GAPDH. The data is shown as ΔCt after normalized to GAPDH. The higher ΔCt the more strand breaks. The P value is shown in the figure. SUPPLEMENT S1 Genes that were significantly UPREGULATED after folate intervention (by unadjusted paired t-test), list is sorted by P value Gene Symbol Nucleotide P VALUE Description OLFM4 NM_006418 0.0000 Homo sapiens differentially expressed in hematopoietic lineages (GW112) mRNA. FMR1NB NM_152578 0.0000 Homo sapiens hypothetical protein FLJ25736 (FLJ25736) mRNA. IFI6 NM_002038 0.0001 Homo sapiens interferon alpha-inducible protein (clone IFI-6-16) (G1P3) transcript variant 1 mRNA. Homo sapiens UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 15 GALNTL5 NM_145292 0.0001 (GALNT15) mRNA. STIM2 NM_020860 0.0001 Homo sapiens stromal interaction molecule 2 (STIM2) mRNA. ZNF645 NM_152577 0.0002 Homo sapiens hypothetical protein FLJ25735 (FLJ25735) mRNA. ATP12A NM_001676 0.0002 Homo sapiens ATPase H+/K+ transporting nongastric alpha polypeptide (ATP12A) mRNA. U1SNRNPBP NM_007020 0.0003 Homo sapiens U1-snRNP binding protein homolog (U1SNRNPBP) transcript variant 1 mRNA. RNF125 NM_017831 0.0004 Homo sapiens ring finger protein 125 (RNF125) mRNA. FMNL1 NM_005892 0.0004 Homo sapiens formin-like (FMNL) mRNA. ISG15 NM_005101 0.0005 Homo sapiens interferon alpha-inducible protein (clone IFI-15K) (G1P2) mRNA. SLC6A14 NM_007231 0.0005 Homo sapiens solute carrier family 6 (neurotransmitter transporter) member 14 (SLC6A14) mRNA. PI3 NM_002638 0.0007 Homo sapiens protease inhibitor 3 skin-derived (SKALP) (PI3) mRNA. SELL NM_000655 0.0008 Homo sapiens selectin L (lymphocyte adhesion molecule 1) (SELL) mRNA. TMPRSS9 NM_182973 0.0008 Homo sapiens polyserase 1 (LOC360200) mRNA. RILPL2 NM_145058 0.0008 Homo sapiens hypothetical protein MGC7036 (MGC7036) mRNA. LOC146206 XM_085383 0.0009 Homo sapiens hypothetical protein LOC146206 (LOC146206) mRNA. OAZ2 NM_002537 0.0009 Homo sapiens ornithine decarboxylase antizyme 2 (OAZ2) mRNA. LCE3C NM_178434 0.0009 Homo sapiens small proline rich-like (epidermal differentiation complex) 3A (SPRL3A) mRNA. CFB NM_001710 0.0011 Homo sapiens B-factor properdin (BF) mRNA. Homo sapiens matrix metalloproteinase 9 (gelatinase B 92kDa gelatinase 92kDa type IV collagenase) MMP9 NM_004994 0.0012 (MMP9) mRNA. LOC151658 XM_379205 0.0012 Homo sapiens hypothetical protein LOC151658 (LOC151658) mRNA. TESC NM_017899 0.0013 Homo sapiens hypothetical protein FLJ20607 (TSC) mRNA. CCDC102B NM_024781 0.0013 Homo sapiens hypothetical protein FLJ23594 (FLJ23594) mRNA. LOC400832 XM_378957 0.0013 Homo sapiens LOC400832 (LOC400832) mRNA. WDFY3 NM_178585 0.0013 Homo sapiens WD repeat and FYVE domain containing 3 (WDFY3) transcript variant 3 mRNA. CCL19 NM_006274 0.0014 Homo sapiens chemokine (C-C motif) ligand 19 (CCL19) mRNA. STK10 NM_005990 0.0014 Homo sapiens serine/threonine kinase 10 (STK10) mRNA. IRX6 NM_024335 0.0015 Homo sapiens iroquois homeobox protein 6 (IRX6) mRNA. ZNF570 NM_144694 0.0015 Homo sapiens hypothetical protein FLJ30791 (FLJ30791) mRNA. LOC388665 XM_371281 0.0016 Homo sapiens similar to DKFZP564K247 protein (LOC388665) mRNA. ADRA1A NM_033303 0.0016 Homo sapiens adrenergic alpha-1A- receptor (ADRA1A) transcript variant 2 mRNA. IL6 NM_000600 0.0017 Homo sapiens interleukin 6 (interferon beta 2) (IL6) mRNA. CCL20 NM_004591 0.0019 Homo sapiens chemokine (C-C motif) ligand 20 (CCL20) mRNA. DUOX2 NM_014080 0.0020 Homo sapiens dual oxidase 2 (DUOX2) mRNA. LOC151534 NM_138482 0.0020 Homo sapiens hypothetical protein BC009264 (LOC151534) mRNA. ANGPT1 NM_139290 0.0020 Homo sapiens angiopoietin 1 (ANGPT1) transcript variant 2 mRNA. GKN2 NM_182536 0.0020 Homo sapiens down-regulated in gastric cancer GDDR (GDDR) mRNA. TGFB3 NM_003239 0.0022 Homo sapiens transforming growth factor beta 3 (TGFB3) mRNA. LOC283547 XM_378454 0.0023 Homo sapiens hypothetical protein LOC283547 (LOC283547) mRNA. ADAMTSL4 NM_019032 0.0023 Homo sapiens thrombospondin repeat containing 1 (TSRC1) mRNA. CD7 NM_006137 0.0024 Homo sapiens CD7 antigen (p41) (CD7) mRNA. C17orf67 XM_378687 0.0025 Homo sapiens hypothetical protein LOC339210 (LOC339210) mRNA. MYR8 XM_028522 0.0025 Homo sapiens myosin heavy chain Myr 8 (MYR8) mRNA. Homo sapiens transcription factor 2 hepatic; LF-B3; variant hepatic nuclear factor (TCF2) transcript variant TCF2 NM_006481 0.0027 b mRNA. WFDC13 NM_172005 0.0027 Homo sapiens WAP four-disulfide core domain 13 (WFDC13) mRNA. LOC283403 XM_211028 0.0027 Homo sapiens hypothetical protein LOC283403 (LOC283403) mRNA. GIMAP5 NM_018384 0.0029 Homo sapiens immune associated nucleotide 4 like 1 (mouse) (IAN4L1) mRNA. KIAA1268 XM_291055 0.0029 Homo sapiens KIAA1268 protein (KIAA1268) mRNA. LOC388198 XM_373655 0.0029 Homo sapiens LOC400485 (LOC388198) mRNA. ECGF1 NM_001953 0.0030 Homo sapiens endothelial cell growth factor 1 (platelet-derived) (ECGF1) mRNA. KIAA0802 XM_031357 0.0030 Homo sapiens KIAA0802 protein (KIAA0802) mRNA. C15orf27 NM_152335 0.0030 Homo sapiens hypothetical protein FLJ38190 (FLJ38190) mRNA. IKBKE NM_014002 0.0030 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells kinase epsilon (IKBKE) mRNA. KIAA1199 NM_018689 0.0031 Homo sapiens KIAA1199 protein (KIAA1199) mRNA. RNF145 NM_144726 0.0032 Homo sapiens hypothetical protein FLJ31951 (FLJ31951) mRNA. CLCNKB NM_000085 0.0033 Homo sapiens chloride channel Kb (CLCNKB) mRNA. MFAP2 NM_017459 0.0033 Homo sapiens microfibrillar-associated protein 2 (MFAP2) transcript variant 1 mRNA. MYH7B XM_371398 0.0033 Homo sapiens myosin heavy polypeptide 7B cardiac muscle beta (MYH7B) mRNA. GRIA2 NM_000826 0.0033 Homo sapiens glutamate receptor ionotropic AMPA 2 (GRIA2) mRNA. LOC124220 NM_145252 0.0035 Homo sapiens similar to common salivary protein 1 (LOC124220) mRNA. PRO0641 NM_014135 0.0035 Homo sapiens PRO0641 protein (PRO0641) mRNA. ZBED2 NM_024508 0.0036 Homo sapiens zinc finger BED domain containing 2 (ZBED2) mRNA. CXCL13 NM_006419 0.0038 Homo sapiens chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) (CXCL13) mRNA. CCR7 NM_001838 0.0039 Homo sapiens chemokine (C-C motif) receptor 7 (CCR7) mRNA. CD1A NM_001763 0.0039 Homo sapiens CD1A antigen a polypeptide (CD1A) mRNA. MICB NM_005931 0.0040 Homo sapiens MHC class I polypeptide-related sequence B (MICB) mRNA. DNMT2 NM_176086 0.0041 Homo sapiens DNA (cytosine-5-)-methyltransferase 2 (DNMT2) transcript variant f mRNA. LOC401051 XM_379174 0.0041 Homo sapiens LOC401051 (LOC401051) mRNA. LOC285192 XM_351525 0.0042 Homo sapiens similar to aquaporin 11 (LOC285192) mRNA. CDH11 NM_033664 0.0043 Homo sapiens cadherin 11 type 2 OB-cadherin (osteoblast) (CDH11) transcript variant 2 mRNA. LOC93082 NM_138397 0.0043 Homo sapiens hypothetical protein BC012317 (LOC93082) mRNA. LGALS2 NM_006498 0.0043 Homo sapiens lectin galactoside-binding soluble 2 (galectin 2) (LGALS2) mRNA. Homo sapiens plectin 1 intermediate filament binding protein 500kDa (PLEC1) transcript variant 11 PLEC1 NM_201384 0.0044 mRNA. SLA2 NM_175077 0.0044 Homo sapiens Src-like-adaptor 2 (SLA2) transcript variant 2 mRNA. Homo sapiens proteasome (prosome macropain) subunit beta type 9 (large multifunctional protease 2) PSMB9 NM_148954 0.0045 (PSMB9) transcript variant 2 mRNA. TRIM7 NM_203296 0.0045 Homo sapiens tripartite motif-containing 7 (TRIM7) transcript variant 3 mRNA. LCN2 NM_005564 0.0045 Homo sapiens lipocalin 2 (oncogene 24p3) (LCN2) mRNA. FCGR3A NM_000569 0.0045 Homo sapiens Fc fragment of IgG low affinity IIIa receptor for (CD16) (FCGR3A) mRNA. EIF5 NM_183004 0.0045 Homo sapiens eukaryotic translation initiation factor 5 (EIF5) transcript variant 2 mRNA. MGC4294 NM_024314 0.0046 Homo sapiens hypothetical protein MGC4294 (MGC4294) mRNA. CD72 NM_001782 0.0047 Homo sapiens CD72 antigen (CD72) mRNA. OVCH2 NM_198185 0.0048 Homo sapiens oviductin protease (OVTN) mRNA. LOC284425 XM_211459 0.0049 Homo sapiens hypothetical LOC284425 (LOC284425) mRNA. TCF7L2 NM_030756 0.0049 Homo sapiens transcription factor 7-like 2 (T-cell specific HMG-box) (TCF7L2) mRNA. RALGPS2 NM_018037 0.0049 Homo sapiens Ral-A exchange factor RalGPS2 (FLJ10244) mRNA. CTLA4 NM_005214 0.0050 Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4) mRNA. C11orf70 NM_032930 0.0050 Homo sapiens hypothetical protein MGC13040 (MGC13040) mRNA. FLJ25344 NM_182512 0.0051 Homo sapiens hypothetical protein FLJ25344 (FLJ25344) mRNA. ADAM21 NM_003813 0.0052 Homo sapiens a disintegrin and metalloproteinase domain 21 (ADAM21) mRNA. KCNJ13 NM_002242 0.0052 Homo sapiens potassium inwardly-rectifying channel subfamily J member 13 (KCNJ13) mRNA. EEF1DP3 NM_145293 0.0052 Homo sapiens similar to hypothetical protein FLJ20897 (LOC196549) mRNA. PAX9 NM_006194 0.0053 Homo sapiens paired box gene 9 (PAX9) mRNA. MEST NM_177525 0.0054 Homo sapiens mesoderm specific transcript homolog (mouse) (MEST) transcript variant 3 mRNA. Homo sapiens fascin homolog 2 actin-bundling protein retinal (Strongylocentrotus purpuratus) (FSCN2) FSCN2 NM_012418 0.0054 mRNA. C21orf96 NM_025143 0.0055 Homo sapiens chromosome 21 open reading frame 96 (C21orf96) mRNA. Homo sapiens myxovirus (influenza virus) resistance 1 interferon-inducible protein p78 (mouse) (MX1) MX1 NM_002462 0.0056 mRNA. KIAA0889 NM_152257 0.0056 Homo sapiens KIAA0889 protein (KIAA0889) mRNA. LOC374344 XM_350798