Jerry M. Troutman, 06/28/2015 1 Jerry M. Troutman University of North Carolina at Charlotte 9201 University City Blvd. Burson 262 Charlotte, NC 28223 Tel: (704) 687-5180 E-mail: [email protected] Website: https://clas-chemistry.uncc.edu/jerry-troutman-research/ EDUCATION University of Kentucky Medical Center, Lexington, KY 09/2006 Ph.D. Biochemistry East Carolina University, Greenville, NC 05/2000 B.S. Biochemistry/Chemistry/Philosophy Cum Laude 2000 PROFESSIONAL Assistant Professor 08/2010-present University of North Carolina at Charlotte, Department of Chemistry NIH Postdoctoral Fellow 09/2008-08/2010 Massachusetts Institute of Technology, Department of Chemistry Advisor: Class of 1922 professor of Chemistry and Biology Barbara Imperiali Postdoctoral Associate 09/2006-09/2008 Massachusetts Institute of Technology, Department of Chemistry Advisor: Class of 1922 Professor of Chemistry and Biology Barbara Imperiali Investigation of bacterial N-linked glycosylation pathways Graduate Research Assistant 08/2000-09/2006 University of Kentucky Medical Center, Department of Molecular and Cellular Biochemistry Advisor: Associate Professor Hans P. Spielmann Isoprenoid selectivity of protein farnesyl transferase a key anti-cancer target HONORS Ruth L. Kirschstein National Research Service Award. 2008-2010 National Institutes of Health General Medicine. (Post-doc, MIT) Research Challenge Trust Fellowship. (Graduate Student, UK) 2001-2002 University Book Exchange Outstanding Senior Scholarship 1999 (Undergraduate, ECU) PROFESSIONAL MEMBERSHIPS American Society for Biochemistry and Molecular Biology (ASBMB) 2012-present Center for Biomedical Engineering and Sciences UNCC 2011-present Molecular Engineering and Design Division American Association for Advancement of Science (AAAS) 2006-present American Chemical Society (ACS) 2004-present Jerry M. Troutman, 06/28/2015 2 EXTERNAL RESEARCH FUNDING Active National Institutes of Health General Medical Sciences AREA grant 04/2015-03/2018 Toolkit development for bacterial isoprenoid utilizing enzymes R15GM114773; Total Award: $350,055 Role: Sole PI National Science Foundation Major Research Instrumentation 08/2013-07/2016 HPLC/ESI-MS System for the analysis of strategic chemical species 1337873; Total Award: $294,955 Role: Co-PI with lead PI: Craig Ogle Co-PIs: Olya Keen, Pinku Mukherjee, John Daniels Completed National Institutes of Health General Medical Sciences AREA grant 04/2012-03/2015 Biosynthesis of the Immunomodulatory Molecule Capsular Polysaccharide A. R15GM100402; Total Award: $320,777 Role: Sole PI INTERNAL RESEARCH FUNDING Completed UNC-Charlotte CLAS Seed Grant 01/2013-12/2013 An integrated approach to redesign Bactoprenyl Diphosphate to be used as a biosynthetic complex carbohydrate scaffold. Co-PI with Donald Jacobs (Physics) Total Award: $5000 UNC-Charlotte CLAS Seed Grant 01/2013-12/2013 Directed self-assembly of supramolecular nanostructure building blocks for optical metamaterials Co-PI with Juan Vivero-Escoto (Chemistry), Marcus Jones (Chemistry), Michael Fiddy (Physics) Total Award: $5000 UNC-Charlotte Faculty Research Grant 01/2011-05/2012 Biosynthesis of a Bacterial Sugar Required for Normal Immune System Function. Total Award: $5773 PEER-REVIEWED JOURNAL PUBLICATIONS Independent Lab * corresponding author Student co-authors under the direction of Dr. Troutman: B.S. Chemistry and/or Biologya, M.S. Chemistryb, Ph.D. Nanoscale Sciencec, Ph.D. Biologyd 8) Sharma, S.,d Erickson, K.M.,c Troutman, J.M.* (2015) Identification and functional characterization of the pyruvyltransferase WcfO from Bacteroides fragilis. In preparation 7) Scott, P.M.,b Milton, A.E.,a Erickson, K.M.,c Troutman, J.M.* (2015) Acetylation of a disaccharide precursor is critical to colanic acid biosynthesis. In preparation 6) Troutman J.M.,* Erickson, K.M.,b,c Scott, P.M.,b Hazel, J.M.,a Martinez, C.D.,a Dodbele, S.b (2015) Tuning the production of variable-length, fluorescent polyisoprenoids using surfactant- controlled enzymatic synthesis. Biochemistry 54, 2817-27. PMID: 25897619 5) Dodbele, S.,b Martinez, C.D.,a Troutman, J.M.* (2014) Species differences in alternative substrate utilization by the anti-bacterial target undecaprenyl pyrophosphate Synthase. Biochemistry 53, 5042-50. PMID: 25020247 4) Troutman, J.M.*, Sharma, S.,d Erickson, K.M.,b,c Martinez, C.D.a (2014) Functional identification of a galactosyltransferase critical to Bacteroides fragilis capsular polysaccharide A biosynthesis. Carbohydrate Research 395, 19-28. PMID: 24997288 Jerry M. Troutman, 06/28/2015 3 3) Mostafavi, A.Z.,b Lujan, D.K.,b Erickson, K.M.,b,c Martinez, C.D.,a Troutman J.M.* (2013) Fluorescent probes for investigation of isoprenoid configuration and size discrimination by bactoprenol-utilizing enzymes. Bioorg Med Chem 21, 5428-35. PMID 23816045 2) Mostafavi, A.Z.,b Troutman J.M.* (2013) Biosynthetic assembly of the Bacteroides fragilis Capsular Polysaccharide A precursor Bactoprenyl-diphosphate-linked Acetamido-4-amino-6- deoxygalactopyranose. Biochemistry 52 1939-49 (Journal Highlight Article) PMID 23458065 1) Lujan, D.K.,b Stanziale, J.A.,a Mostafavi, A.Z.,b Sharma, S.,d Troutman, J.M.* (2012) Chemoenzymatic synthesis of an isoprenoid phosphate for the analysis of complex bacterial oligosaccharide biosynthesis. Carbohydrate Research 359, 44-53. (Also, highlighted and re- published in Carbohydrate Research virtual special issue published online in 2013) PMID:22925763 Prior to UNC-Charlotte refereed publications 12) Subramanian, T., Pais, J.E., Liu, S., Troutman, J.M., Suzuki, Y., Subramanian, K.L., Fierke, C. Andres, D.A., Spielmann, H.P.* (2012) Farnesyl Diphosphate analogues with aryl moieties are efficient alternate substrates for protein farnesyltransferase. Biochemistry 51, 8307-19. PMID: 22989235 11) Morrison, J.P., Troutman, J.M., Imperiali B.* (2010) Development of a multicomponent kinetic assay of the early enzymes in the Campylobacter jejuni N-linked glycosylation pathway. Bioorg Med Chem 18, 8167-71. PMCID: PMC2981441 10) Adams, V.R., Deremer, D.L., Stevich, B., Mattingly, C.A., Gallt, B., Subramanian, T., Troutman, J.M., Spielmann, H.P.* (2010) Anticancer activity of novel unnatural synthetic isoprenoids. Anticancer Research 30, 2505-12. PMID: 20682975 9) Troutman, J.M., Imperiali, B.* (2009) Campylobacter jejuni PglH is a Single Active Site Processive Polymerase that Utilizes Product Inhibition to Limit Sequential Glycosyl Transfer Reactions. Biochemistry 48, 2807-2816. PMCID: PMC2736683 8) Subramanian, T., Liu, S., Troutman, J.M., Andres, D.A., Spielmann H.P.* (2008) Protein farnesyltransferase catalyzed isoprenoid transfer to peptide depends on lipid size and shape not hydrophobicity. Chembiochem 9, 2872-82. PMID: 18985644 7) Troutman, J. M., Subramanian, T., Andres, D. A., and Spielmann, H. P.* (2007) Selective modification of CaaX peptides with ortho-substituted anilinogeranyl lipids by protein farnesyl transferase: competitive substrates and potent inhibitors from a library of farnesyl diphosphate analogues. Biochemistry 46, 11310-21. PMID: 17854205 6) Troutman, J. M., Andres, D. A., and Spielmann, H. P.* (2007) Protein farnesyl transferase target selectivity is dependent upon peptide stimulated product release. Biochemistry 46, 11299- 309. PMID: 17877368 5) Roberts, M. J., Troutman, J. M., Chehade, K. A., Cha, H. C., Kao, J. P., Huang, X., Zhan, C. G., Peterson, Y. K., Subramanian, T., Kamalakkannan, S., Andres, D. A., and Spielmann, H. P.* (2006) Hydrophilic Anilinogeranyl Diphosphate Prenyl Analogues Are Ras Function Inhibitors. Biochemistry 45, 15862-15872. PMID: 17176109 4) Troutman, J. M., Roberts, M. J., Andres, D. A., and Spielmann, H. P.* (2005) Tools to analyze protein farnesylation in cells. Bioconjug Chem 16, 1209-17. PMID: 16173800 3) Subramanian, T., Wang, Z., Troutman, J. M., Andres, D. A., and Spielmann, H. P.*(2005) Directed library of anilinogeranyl analogues of farnesyl diphosphate via mixed solid- and solution-phase synthesis.Org Lett 7, 2109-12. PMID: 15901146 Jerry M. Troutman, 06/28/2015 4 2) Troutman, J. M., Chehade, K. A., Kiegiel, K., Andres, D. A., and Spielmann, H. P.* (2004) Synthesis of acyloxymethyl ester prodrugs of the transferable protein farnesyl transferase substrate farnesyl methylenediphosphonate. Bioorg Med Chem Lett 14, 4979-82. PMID: 15341963 1) Kelly, M. A., Chellgren, B. W., Rucker, A. L., Troutman, J. M., Fried, M. G., Miller, A. F., and Creamer, T. P.* (2001) Host-guest study of left-handed polyproline II helix formation. Biochemistry 40, 14376-83. NATIONAL CONFERENCE PRESENTATIONS Presenting author in bold 22) Scott, P.M., Troutman, J.M. (2015) Colanic acid biosynthesis pathway elucidation using fluorescent bactoprenols. Carbohydrates Gordon Conference West Dover, VT. 06/14/2015- 06/19/2015. (poster/talk) 21) Erickson, K.M., Hazel, J.M., Troutman, J.M. (2015) Characterization of the hexose-1- phosphate transferase from the Vibrio vulnificus capsular polysaccharide biosynthesis pathway. Carbohydrates Gordon Conference West Dover, VT. 06/14/2015-06/19/2015. (poster) 20) Scott, P.M., Troutman J.M. (2015) Characterization of the colanic acid biosynthesis pathway with a novel isoprenoid analogue. 52-BIOL ACS National Conference, Denver, CO 3/22/15 (poster) 19) Hazel, J.M., Erickson, K.M., Chahoud, M.A.,Troutman J.M. (2015) Study and Characterization of the sugar-modifying enzymes of the capsule biosynthesis pathway of Vibrio Vulnificus. 66-BIOL. ACS National Conference, Denver, CO 3/22/15 (poster) 18) Erickson, K.M., Hazel, J.M., Troutman, J.M. (2015)