View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Kyoto University Research Information Repository A New Species of Pachytriton from China (Amphibia: Urodela: Title Salamandridae) Author(s) Nishikawa, Kanto; Matsui, Masafumi; Jiang, Jian-Ping Citation Current Herpetology (2012), 31(1): 21-27 Issue Date 2012-06 URL http://hdl.handle.net/2433/216840 Right © 2012 by The Herpetological Society of Japan Type Journal Article Textversion publisher Kyoto University Current Herpetology 31(1): 21–27, June 2012 doi 10.5358/hsj.31.21 © 2012 by The Herpetological Society of Japan A New Species of Pachytriton from China (Amphibia: Urodela: Salamandridae) 1 1 2 KANTO NISHIKAWA *, MASAFUMI MATSUI , AND JIAN-PING JIANG 1 Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606–8501, Japan 2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China Abstract: A new species of the salamandrid genus Pachytriton is described based on two individuals purchased from pet shops in Japan. The original locality of these specimens is known only as “China”, and further details are not known. Morphologically, this species differs from all other congeners in the combination of coloration, body size, snout length, head width, tail length and width, and length of upper jaw tooth series and vomerine tooth series. Genetically, this species is separated from all other congeners by substantial genetic distances in mitochondrial DNA sequences. Key words: China; DNA bar-coding; New species; Pachytriton; Pet trade INTRODUCTION After these specimens were obtained from two pet shops in Japan in 2007, we tried to The salamandrid genus Pachytriton Bou- discover their origin by visiting China every lenger, 1878, occurs in eastern and southeast- year to collect specimens in the wild, but with- ern China (Fei et al., 2006), and currently out success. Therefore, we decided to describe consists of six nominal species, P. archospotus, the specimens here as a new species, although P. brevipes, P. feii, P. granulosus, P. inexpec- the number of specimens is small and their tatus, and P. moi (Nishikawa et al., 2011a). exact locality is unknown. A complete sequence Based on molecular and morphological analy- of the cytochrome b (hereafter, cyt b) gene of ses, Nishikawa et al. (2011a) recognized three mitochondrial DNA (mtDNA) of the new unnamed lineages in the genus Pachytriton species has been deposited in GenBank (acces- and described two of them as P. feii and P. sion numbers: AB638709 and AB638711). moi. However, Nishikawa et al. (2011a) did Consequently, future studies will be able to not name the single remaining lineage in their detect wild populations of this new species by paper, because it was represented by only two comparing these GenBank sequences with specimens imported from China and obtained those of wild specimens collected. from Japanese pet shops, without any more detailed locality information. MATERIALS AND METHODS * Corresponding author. TEL: +81–75–753–6848; We compared the morphology of two adult Fax: +81–75–753–2891; male specimens of Pachytriton obtained in E-mail address: [email protected] two pet shops in Japan with that of the six 22 Current Herpetol. 31(1) 2012 TABLE 1. Measurements of types and other specimens of Pachytriton examined (means±SD of SVL 〈in mm〉 and medians of ratios of characters 〈R: %SVL〉 and VTL/VTW, with ranges in parentheses). H: holotype, P: paratype, S: syntype, T: topotype. For character abbreviations, refer to text. Species changi sp. nov. archospotus brevipes feii granulosus inexpectatus moi Types included H P 2P S H, 17P 9T H, 2P, 11T H n1131189141 SVL 84.2 81.8 86.8±5.9 87.6 73.3±8.6 69.0±6.1 87.8±10.1 100.2 (81.5–93.1) (58.5–90.5) (59.0–78.9) (68.6–102.1) RHL 25.2 25.4 25.5 25.6 27.1 26.6 28.7 35.9 (23.5–29.4) (24.5–29.1) (24.5–29.0) (24.6–31.6) RHW 18.8 18.2 21.7 19.6 20.0 19.0 19.8 23.2 (21.7–22.2) (18.0–21.4) (17.0–20.3) (18.5–21.7) RMXHW 20.5 19.3 24.3 21.9 21.2 20.0 22.3 25.3 (23.8–24.6) (19.9–22.6) (17.9–21.5) (20.7–25.2) RSL 10.1 9.8 8.0 8.4 9.7 8.5 9.5 11.3 (7.8–8.3) (8.1–10.8) (7.9–9.6) (8.6–10.6) RLJL 11.9 12.6 11.5 12.0 13.1 12.9 13.5 17.5 (11.0–12.0) (10.7–14.2) (11.7–13.8) (11.6–14.9) RENL 7.2 7.3 5.7 6.6 7.2 6.6 7.0 8.6 (5.4–5.9) (6.3–8.2) (6.2–7.4) (6.4–7.9) RIND 7.1 6.8 5.0 5.3 6.3 6.0 6.5 7.5 (4.9–5.8) (5.7–7.4) (4.6–6.4) (5.9–7.4) RIOD 7.7 7.0 9.3 8.6 7.6 6.9 6.9 8.0 (8.8–9.6) (6.9–9.3) (6.1–8.2) (6.4–7.9) RUEW 2.4 2.9 1.5 2.4 2.7 3.3 2.5 2.7 (1.2–1.6) (1.9–3.8) (3.0–4.5) (2.0–3.2) RUEL 4.5 4.9 3.8 4.8 5.2 6.1 4.9 4.7 (3.7–4.2) (4.3–6.7) (4.6–6.8) (3.9–6.1) ROL 3.7 3.0 2.6 3.5 3.6 3.0 2.9 3.7 (2.1–2.8) (2.8–4.1) (2.7–3.7) (2.4–3.5) RAGD 51.7 48.5 50.1 51.6 50.1 51.7 49.5 52.1 (49.2–50.9) (45.7–52.3) (48.6–52.9) (43.7–53.7) RTRL 74.8 74.5 74.5 74.4 72.9 73.4 71.3 64.1 (70.6–76.5) (70.9–75.5) (71.0–75.5) (68.4–75.4) RTAL 105.1 101.1 95.7 101.1 97.4 101.6 90.6 90.5 (93.5–95.8) (87.7–105.4) (94.7–104.8) (85.4–98.7) RVL 5.7 6.1 5.0 7.2 7.3 8.3 5.6 5.2 (4.8–5.0) (4.7–8.7) (5.3–9.4) (4.6–7.5) RBTAW 12.0 12.2 15.8 14.3 12.4 14.7 14.7 13.2 (15.7–17.2) (10.6–14.3) (14.0–15.7) (12.3–16.5) RMTAW 7.5 8.3 9.8 10.0 8.9 12.0 11.2 7.8 (8.6–12.5) (7.5–10.5) (10.7–15.0) (8.9–13.5) RBTAH 10.0 10.8 15.9 12.9 11.5 12.9 12.2 12.8 (14.0–16.0) (10.3–14.2) (11.4–13.5) (10.3–14.2) NISHIKAWA ET AL.—NEW NEWT FROM CHINA 23 RMXTAH 15.4 15.0 17.5 17.1 15.6 16.2 14.9 14.4 (16.1–18.0) (13.0–16.6) (13.3–19.1) (12.4–15.7) RMTAH 15.2 14.7 16.4 15.9 14.9 12.9 13.5 14.3 (15.0–17.3) (12.9–16.0) (11.8–16.5) (10.8–14.6) RFLL 24.5 23.3 23.3 22.0 25.8 23.2 20.3 22.7 (21.7–24.3) (22.9–29.4) (20.7–23.6) (18.5–23.6) RHLL 26.5 27.8 29.4 25.9 29.6 25.3 24.5 24.4 (25.9–29.6) (26.1–32.5) (23.4–27.4) (21.8–35.3) RUJTW 11.2 10.6 9.4 8.7 9.5 9.3 9.8 13.3 (8.2–9.8) (7.8–11.4) (7.9–10.1) (8.2–10.9) RUJTL 9.1 8.6 5.9 6.3 6.7 6.3 7.9 10.1 (5.2–6.5) (5.1–8.4) 94.6–7.1) (7.4–8.6) RVTW 6.2 5.6 5.8 3.9 5.9 4.2 4.6 6.8 (5.0–6.0) (4.9–8.2) (3.6–5.6) (4.0–5.5) RVTL 12.1 11.0 8.3 7.8 9.7 8.9 11.7 16.5 (8.2–9.0) (7.6–11.0) (7.4–10.9) (10.4–13.0) VTL/VTW 2.0 2.0 1.5 2.0 1.6 2.1 2.4 2.4 (1.4–1.7) (1.3–2.0) (1.7–2.4) (2.1–2.9) nominal species of the genus (Table 1, anterior tip of vent; TAL (tail length): from Appendix I), including type specimens of P. anterior tip of vent to tail tip; VL (vent length): archospotus, P. brevipes, P. feii, P. inexpec- from anterior to posterior tip of vent; BTAW tatus, and P. moi, and topotypic specimens of (basal tail width): tail width measured at base P.